For example, here is a run with a failure to detect objects in column 3 for the first 2 rows (A3 and B3 failed). The wells
are shown in red and the error status is displayed. An alert email was sent as entered in the plate definition.
During a Run
l
You may stop the plate at any time by clicking the Stop button. This does not initiate sterilize (even if the Ster-
ilize after running plate box is checked).
l
Should the sheath tank or beads reservoir become empty or the waste tank full during a run, an alert will be
sent to the email entered in the well plate definition. Acquisition will pause until the user intervenes.
l
If an error occurs on a well, the sample is returned, an alert is sent to the email address entered in the well
plate definition, and the AutoSampler moves on to the next well.
l
If the same error occurs on three consecutive wells, the AutoSampler aborts the plate and sterilizes the instru-
ment (if the "Sterilize after running plate" box is checked)
Report
l
A well plate report (.txt file) will be saved to the folder designated in the Output File Path of the plate definition
at the end of the run either when it was stopped manually or completed the entire plate.
l
If batching was included in the well plate definition, the data files will be processed using the IDEAS
®
Software
compensation matrix and templates designated. All of the .cif, .daf and statistics report .txt files will be saved to
the designated output file path.
Notes
Email notifications: The parameter 'Error Notification Email' may be selected. Provide the email address for notification.
In order for this to work, the system must be enabled as follows.
1. From the Instrument menu, choose Options.
2. Select Email Notification tab.
For Research Use Only. Not for use in diagnostic procedures.
37
Amnis
®
FlowSight
®
Imaging Flow Cytometer User Manual
