Luminex Amnis FlowSight, Руководство пользователя

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Data Acquisition
After the FlowSight
®
System is calibrated, you are ready to acquire experiment data files. The sample is loaded into the
sample pump. Sample is injected into the flow cell to form a single core stream that is hydrodynamically focused in front
of the imaging objective. Refer to the AutoSampler upgrade section below for using unattended operation.
Refer to the Sample Preparation Guide or see Experimental Design for recommendations. Use compatible sample solu-
tions from the table below.
Sample Solution Sheath Fluid Acceptable
PBS PBS Yes
PBS Water Yes*
PBS/Surfactant PBS Yes
PBS/Surfactant Water No
Water PBS Yes
Water Water Yes
Water/Surfactant PBS No
Water/Surfactant Water Yes
*Cells in PBS run with water sheath will swell.
Sample Order
Samples from an experiment are typically run in the following order:
l
Experimental sample with the brightest stains to set the sensitivity for the run
l
Single color DNA dye control NO BF or SSC to ensure correct dye concentration
l
10% bleach to wash out DNA dye followed by PBS
l
Single color fluorescence compensation samples (no DNA dye) NO BF or SSC, all channels enabled
l
The rest of the experimental samples with DNA dye (if applicable)
Loading and Running the Sample
1. Choose Load Default Template from the File menu.
2. Press Load, and load an aliquot of the brightest sample in the experiment, that fluoresces with each fluorochrome
used. It is critical that you run this sample first to establish the instrument settings. (DO NOT change laser settings
for the experiment once established on this sample.)
NOTE: Application-specific instrument settings can be saved in a template and used to facilitate instrument setup,
but it is recommended that you verify the appropriateness of the settings for the specific experimental run.
For Research Use Only. Not for use in diagnostic procedures.
19
Amnis
®
FlowSight
®
Imaging Flow Cytometer User Manual