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2.3.1 Settings: Staining Options
Users can select for the use or absence of a stain for cell counting with the LUNA-II
™
. The LUNA-II
™
is optimized for use
with trypan blue or Erythrosin B.
Option Description
With Trypan Blue
This option is used when cell samples are mixed with trypan blue or Erythrosin B for regular
bright field counting. This option generates cell viability data.
Adjust the dilution factor in your set protocol appropriately.
*
Without Trypan Blue
**
When samples are not mixed with a stain, select this option and follow the directions in the
message boxes.
Adjust the dilution factor in your set protocol appropriately.
*
*
The dilution factor will not change automatically. Failure to adjust the dilution factor will lead to inaccurate cell concentration calculations.
**
Low contrast from not using a stain may lead to suboptimal results.
Press [Staining Options] in the Settings screen.
The selected option will be marked with a blue .
Change the staining option by pressing the unselected option.
Select With Trypan Blue when using a stain such as trypan blue or Erythrosin B.

Important! Users must recalibrate the background when switching stains (see Section 2.3.5: Settings:
Background Calibration).
Press OK to select the desired staining option. Otherwise, press Cancel to close the window.
Press < to return to the Settings screen.
Choose the appropriate protocol or adjust the dilution factor accordingly (see Section 3.1: Protocol Parameters and
Section 3.2. Creating and Editing Protocols).