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6.4 Troubleshooting
Problem
Possible Cause
Solution
Inaccurate cell count
Clumped cells
Gently but thoroughly pipette your cell suspension to break up
aggregates prior to counting. Alternately, increase trypsinization time.
Too few or too many cells
Cell concentrations of 5 x 10
4
-1 x 10
7
cells/mL are optimal for counting.
Dilute or concentrate cell suspensions accordingly.
Improper slide insertion
Make sure that the slide has been inserted into the instrument.
Improper sample loading
Do not over- or under-fill the slide chambers. Carefully load the
chambers with 10-12 µL of cell suspension.
Malfunction of optical
components
Optical components may be dirty or damaged. Please contact your
local distributor or Logos Biosystems.
Damaged or
contaminated slide
Use a new LUNA
™
Cell Counting Slide or clean the LUNA
™
Reusable
Slide and its coverslips thoroughly before use. Wear gloves and handle
by the edges to avoid smudging and contamination.
Incorrect dilution factor
Adjust the dilution factor in the selected protocol or create a new
protocol. Make sure the appropriate staining option has been selected.
Data transfer and
saving
Incompatible USB drive
Some USB devices are undetectable or incompatible. Use the USB
supplied with the instrument or use a USB 2.0.
Too many files in the
USB drive
Delete or transfer files.
Background
calibration taking too
long
Freezing during
background calibration
If calibration takes more than 10 minutes, reset the system by turning
the power off and then on again. Contact your local distributor or Logos
Biosystems if calibration fails repeatedly.
Errors while
updating software
Incompatible USB drive
Some USB devices are undetectable or incompatible. Use the USB
supplied with the instrument or use a USB 2.0.
More than one software
version on the USB drive
Delete previous versions of software from the USB drive before
downloading new software.
Incorrectly saved or
damaged software
Use the supplied or a compatible USB drive. Download the file again
into the root directory of the USB drive. Insert the USB and press
[Software Updates] in the Settings screen. If the problem persists,
contact your local distributor or Logos Biosystems.
LED on printer
flashing
Paper receptacle empty
Check to see there is enough paper in the printer. If there is not, replace
it (see Section 6.3: Installing Printer Paper). If the flashing persists,
contact your local distributor or Logos Biosystems.