10
2.3.5 Settings: Background Calibration
Background calibration adjusts for the specific shade of the stain used for counting and is a prerequisite for the
successful detection of cells. Users must recalibrate the background after each software update or when using a
different brand, concentration, or type of stain. The LUNA-II
™
is optimized for use with trypan blue or Erythrosin B.
Press
[Background Calibration]
in the Settings screen.
A window will appear with directions for Calibration Step 1.
The counting slide port should be empty for Calibration Step 1. If there is a slide in the counting slide port, remove it from
the instrument.
Press
Start
.
Do not turn the instrument off during this process.
Mix one part stain with an equal volume of distilled water, PBS, or plain medium. Put 10 µL of the diluted stain into the
chamber of a new LUNA
™
Cell Counting Slide or a clean LUNA
™
Reusable Slide.
A window will appear with directions for Calibration Step 2 when Calibration Step 1 is complete.
Insert the slide face up and sample-side first into the counting slide port.
Important!
Do not insert the slide facedown.
Press
Start
. Do not remove the slide or turn off the instrument during this process.
Press
Exit
to return to the Settings screen when Calibration Step 2 is complete. The background calibration value and
date will have changed in the Settings Screen.