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4.5 Cell Counting
Use a finger or a stylus to navigate the image. The red outer box in the top left corner of the image represents the entire
counting area and the inner box is the current field of view. The size and location of the inner box will change with the
magnification and movement of the screen
Press the magnifier button to zoom in and out of the image.
Press
[Count]
to start counting.
The LUNA-II
™
counts the cells in 0.5 µL, which is comparable to five (1 mm x 1 mm) squares on a standard
hemocytometer.
Counting time will vary with protocol, cell size, and cell concentration. With the DEFAULT protocol, cell samples with a
concentration of ~1 x 10
6
cell/mL will take at minimum 10 seconds to count without autofocusing or 15 seconds with
autofocusing.
Cell count and viability results will appear.