CONTENTS
6.
Troubleshooting ......................................................... 86
7.
Care of the Microscope ............................................ 89
7.1
Dust Cover ................................................................... 89
7.2
Cleaning ....................................................................... 89
7.3
Handling acids and bases ........................................ 90
8.
Essential Consumable and Spare Parts ............... 91
9.
Appendix .................................................................... 92
9.1
Assigning the variable function keys ..................... 92
9.2
Abbreviation glossary ............................................... 94
9.3
Overview of Touch Screen pictograms .................. 95
9.4
Additional information ............................................... 96
10.
Index ............................................................................ 97
11.
EC Declaration of Conformity .................................. 99
5.
Operation .................................................................... 38
5.1
Switching on the microscope .................................. 39
5.2
Illumination ................................................................. 41
5.2.1
Transmitted Light........................................................ 41
5.2.2
Fluorescence .............................................................. 42
5.2.3
Infinity port .................................................................. 44
5.2.4
Shutter ......................................................................... 45
5.3
Objectives ................................................................... 46
5.4
Tube .............................................................................. 49
5.5
Eyepieces .................................................................... 50
5.6
Stages and object displacement ............................. 51
5.6.1
Object displacement ................................................. 51
5.6.2
Adjusting the step sizes on motorized stages ....... 52
5.7
Focusing ...................................................................... 54
5.7.1
Focusing the image ................................................... 54
5.7.2
Focus thresholds ........................................................ 55
5.7.3
Adjusting the step sizes ............................................ 56
5.7.4
AFC (Adaptive Focus Control) .................................. 57
5.8
Selecting the ports .................................................... 58
5.9
Magnification Changer ............................................. 59
5.10
Aperture diaphragm and field diaphragm ............. 60
5.11
Filter ............................................................................. 62
5.11.1 Transmitted Light filter .............................................. 62
5.11.2 Fluorescence Cubes .................................................. 63
5.11.3 Leica RFID Module......................................................... 65
5.11.3.1 Product Description ................................................. 65
5.11.3.2 Specifications LID Module 1.0 ...............................65
5.11.3.3 Specifications RFID Tag D6.7 special ..................... 65
5.11.3.4 Ports ............................................................................. 65
5.11.3.5 FCC / IC Compliance .................................................. 65
5.12
The memory function on the Touch Screen .......... 66
5.13
Transmitted Light contrast method ......................... 66
5.13.1 Brightfield (TL) ............................................................ 67
5.13.2 Phase Contrast (TL) ................................................... 69
5.13.3 Darkfield (TL) .............................................................. 71
5.13.4 Polarization (TL) ......................................................... 72
5.13.5 Differential Interference Contrast – DIC (TL) ....... 74
5.13.6 Integrated Phase Contrast – IPH (TL) .................... 76
5.13.7 Integrated Modulation Contrast – IMC (TL) .......... 78
5.14
Fluorescence .............................................................. 82
5.15
Combination method ............................................... 85
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