51
7. Startup
7.6.2 Incident Light
For each objective there are reasonable values
assigned to aperture and field diaphragm. The
incident light module is also pre-centered at the
factory.
Due to transportation and setup of the stand,
however, you might have to re-center the inci-
dent light module. Please check the aperture
and field diaphragm centering.
The following procedure refers to the incident
light bright field illumination.
• Select an objective with moderate magnifica-
tion (10x-20x)
• If necessary, activate the incident light axis by
pressing the TL/IL key (43.1).
• Activate the bright field contrasting method by
pressing IL-BF/Fluo (one of the variable func-
tion keys at the stand, STP6000 or at the Leica
SmartTouch).
• Insert the specimen in the stage’s specimen
holder (45.1).
• Focus on the specimen with the SmartMove,
STP6000 or focus wheel.
• Adjust the light intensity with the
INT
keys
(43.3).
Adjusting the field diaphragm
• Close the field diaphragm with the
FD
keys
(43.4) until the edge of the diaphragm (round
or rectangular) appears on the specimen
plane.
• If the boundaries of the field diaphragm are
not in the center of the field of view, the dia-
phragm has to be moved with the two
centering bolts (48a.1) on the right side of the
stand.
• With the function keys
FD
(43.4) on the left
side of the stand, open the field diaphragm un-
til it just disappears from the field of view.
• When using a digital camera, rectangular field
diaphragms are recommended. Adjust the
size of the diaphragm to the chip size of your
camera.
Fig. 48a
Adjusting the field diaphragm in the incident light
axis
1
Centering bolts for moving the field diaphragm
1
Fig. 48b
Adjusting the aperture diaphragm in the incident
light axis
1
Centering bolts for moving the aperture diaphragm
1
Содержание DM5500 B
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