DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
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HPLC Glossary
retention time
The time required from an injection of a substance until the maximum
concentration of a substance becomes visible.
sample
A mixture of different components which are to be separated via chroma-
tography. The components are moved by the mobile phase and dissolved
from the column.
sample loop
A loop which is separate from a chromatographic system and which the
sample is injected to. After a switch is actuated, the solvent flow passes
the loop and the sample is flushed onto the column.
solute
The dissolved component of a mixture that is to be separated in the chro-
matographic column.
solvent
The liquid used to dissolve a sample for the injection into a chromatogra-
phy column or CE capillary.
stationary phase
The immobile phase involved in the chromatographic process. It is the
liquid or the liquid film on the surface of the packing material.
Term
Definition
