Knauer Axura DAD 2.1L, Руководство пользователя

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DAD 6.1L/DAD 2.1L/MWD 2.1L user manual V6700, version 3.2
HPLC Glossary 55
HPLC Glossary
In the following chapter you find abbreviations and terminology that is used
in HPLC.
.
Term Definition
absorption The process of retention in which the solute partitions into a liquid-like
coating.
adsorption A process of retention in which the interactions between the solute an
dthe surface of an adsorbent dominate.
analytical Qualitative analysis of samples in HPLC
backflushing Useful in chromatography to remove compounds that are held strongly
at the head of a column.
calibration A process for correcting measuring values by the value that a measuring
device deviates from the standard.
capillary Tubing to connect various parts of the chromatograph in order to direct
flow to the proper place.
carrier Refers to the support that is used to attach the active ligand, usually by a
covalent bond.
chromatogram A plot of detector signal output versus time or elution volume during the
chromatographic process.
column The tube and stationary phase through which mobile phase flows result-
ing in a chromatographic separation.
correction factor factor that arithmetically corrects device-related deviations from measur-
ing values
dead volume Dead volume is extra volume experienced by solutes as they pass through
a chromatographic system, in particular any unswept volume exposed to
the mobile phase flow.
degassing The process of removing dissolved gas from the mobile phase prior or
during use.
detector device measuring the composition or the quantity of a substance
gradient A process to change solvent strength as a function of time (normally sol-
vent strength increases) thereby eluting progressively more highly
retained analytes.
isocratic mode of sample separation where the composition of a solvent remains
constant
Luer-Lock a standardized connector between syringes and cannulas
mobile phase The fluid that moves solutes through the column.
packing The adsorbent, gel, or solid support used in the chromatography column.
preparative Isolating the maximum amount of a substance in a short amount of time
in a required purity
response time Time for a detector to respond to ~90 % of the incoming solute amount.
The response time is generally taken as 2–4 times the time constant.