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iQue

Screener PLUS Hardware Manual: Part Number 11811 Rev B, Getting to Know the iQue Screener PLUS

Fluorescence Channels - VBR

The iQue Screener PLUS–VBR has three lasers and thirteen fluorescence channels. The table below lists these

channels, their associated filter, and gives a few example dyes for each.

Channel Position

Filter

Example Dyes

Violet Laser (405nm)

VL1

445/45

Pacific Blue, BV421, DAPI

VL2

530/30

Alexa Fluor 430-H, BV510, Qdot525

VL3

572/28

Pacific Orange, BV570, Qdot585

VL4

615/24

BV605, Qdot605

VL5

675/30

BV650/711, Qdot655

VL6

780/60

BV785, Qdot800

Blue Laser (488nm)

BL1

530/30

FITC, Alexa 488, GFP

BL2

572/28

PI, PE

BL3

615/24

Texas Red, eFluor 615

BL4

675/30

PE-cy5.5, PerCP, 7-AAD

BL5

780/60

PE-cy7

ed Laser (6

40nm)

RL1

675/30

APC, DRAQ5/7

RL2

780/60

APC-cy7, APC - eFluor 780

Содержание iQue Screener PLUS

Страница 1: ...iQue ScreenerPLUS hardware manual December 2016...

Страница 2: ......

Страница 3: ...cautions 11 General Warnings 11 Operator Safety 12 Safety Symbols and Labels 13 Biological Handling Precautions 14 Laser Precautions 14 Operational Precautions 15 Electrical Precautions 15 GETTING TO...

Страница 4: ...1 MAINTENANCE 55 Recommended Maintenance Schedule 57 Standard Cleaning Procedures 59 Changing the Probe and Tubing 63 Fluidics Maintenance 69 Managing Plate Models 75 Manual Control Mode 83 Clog Preve...

Страница 5: ...otographic mechanical or otherwise for any purpose without the express written permission of IntelliCyt Corporation Trademarks IntelliCyt HTFC iQue ForeCyt Screening Solutions for Life and iDM are tra...

Страница 6: ...may accept the Product AS IS or return the Product and receive a refund of fees paid for the Product 6 Limited Warranties Exclusion of Certain Warranties 6 1 Limited Warranty IntelliCyt warrants that...

Страница 7: ...r shall not assign any of its rights or obligations under this Agreement without IntelliCyt s prior express written consent which may be granted or withheld at IntelliCyt s sole discretion Any attempt...

Страница 8: ...NSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY WHETHER FOR BREACH OF CONTRACT TORT INCLUDING NEGLIGENCE OR OTHERWISE ARISING OUT OF OR RELATED TO THIS AGREEMENT INCLUDING BUT NOT LI...

Страница 9: ...5 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Introduction Declaration of Conformity...

Страница 10: ...6 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Introduction THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 11: ...one quick motion Double click Place the mouse pointer over the item then depress and release the primary mouse button twice in quick succession Drag Place the mouse pointer over the item depress and...

Страница 12: ...identify the nature of any potential hazard and determine the actions to perform CAUTION A cautionary statement which if not followed may result in instrument damage or corruption of data Risk of Fire...

Страница 13: ...e at 505 345 9075 Opt 2 Before contacting us please be prepared to answer the following questions What were you doing when the problem occurred Did you try to follow the steps in this guide but they d...

Страница 14: ...10 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Using This Manual THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 15: ...n of the iQue Screener PLUS the system must be connected to an IntelliCyt approved uninterruptable power supply General Warnings THE iQue SCREENER PLUS IS INTENDED FOR GENERAL LABORATORY AND RESEARCH...

Страница 16: ...hooting Take full advantage of all safety features and do not defeat safety interlocks and or sensors Acknowledge and act upon instrument alarms and error messages IF THIS INSTRUMENT IS USED IN A MANN...

Страница 17: ...threatening Wear appropriate laboratory attire follow universal laboratory safety protocols and adhere to local regulations Electrical Shock Hazard There is the possibility of electrical shock when t...

Страница 18: ...or when prompted by software to prevent spillover and possible biohazard risk Consult appropriate Material Safety Data Sheets when necessary The screening system is programmed for automatic decontami...

Страница 19: ...including sample tubes and racks Before manually cleaning the system turn off the instrument and unplug the power cord Electrical Precautions Electrical devices pose the risk of an electric shock To...

Страница 20: ...16 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Safety THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 21: ...must be plugged into an IntelliCyt approved uninterruptable power supply UPS The UPS must be plugged into a standard grounded or earthed mains electrical outlet conforming to local codes Non grounded...

Страница 22: ...ticolor Detection BR VBR VYB 6 Simultaneous Color Channels using PMTs 13 Simultaneous Color Channels using PMTs 13 Simultaneous Color Channels using PMTs Label Free Object Detection 2 Light Scatter Ch...

Страница 23: ...lasers and six fluorescence channels The table below lists these channels their associated filter and gives a few example dyes for each Channel Position Filter Example Dyes Blue Laser 488nm BL1 530 30...

Страница 24: ...le dyes for each Channel Position Filter Example Dyes Violet Laser 405nm VL1 445 45 Pacific Blue BV421 DAPI VL2 530 30 Alexa Fluor 430 H BV510 Qdot525 VL3 572 28 Pacific Orange BV570 Qdot585 VL4 615 2...

Страница 25: ...osition Filter Example Dyes Violet Laser 405nm VL1 445 45 Pacific Blue BV421 DAPI VL2 530 30 Alexa Fluor 430 H BV510 Qdot525 VL3 586 20 Pacific Orange BV570 Qdot585 VL4 615 20 BV605 Qdot605 VL5 660 20...

Страница 26: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Getting to Know the iQue Screener PLUS 22 Front View Lid Sampling Area Shaker Status Light Light Switch...

Страница 27: ...ontroller window will match the physical status light Green Ready Yellow Warning Red Not Ready The iQue Screener PLUS will continue to run if it encounters a warning For example a Waste Bottle is clos...

Страница 28: ...umber 11811 Rev B Getting to Know the iQue Screener PLUS 24 Shaker Detail Shaker Deck Gripper Release Lever Gripper Pins 2 of 4 Shaker Body Sampling Area Detail Shaker Cable Guide Microcentrifuge Tube...

Страница 29: ...LUS Sampler Detail Contact Sensor Data Cable Sample Tubing Probe Locking Nut Contact Sensing Probe Assembly Probe Guide Z Arm Tubing Pass Through XY Arm Outer Probe Inner Probe Pump Detail Peristaltic...

Страница 30: ...811 Rev B Getting to Know the iQue Screener PLUS 26 Right Side Panel Detail Fluidics Station Data Connection Sheath Port Waste Port PLUS One Port PLUS Two Port Bottle Station Sheath Bottle PLUS One Bo...

Страница 31: ...11811 Rev B Getting to Know the iQue Screener PLUS Fluidics Harness Detail Fluid Station Data Cable Auto closing Quick Connects Fluid Line Labels Harness Direction Label Back Panel Detail Fuse Holder...

Страница 32: ...S 28 Front Service Area Fluidlink Connection Area Sheath Dampener Sheath Filter Sheath Pump CAUTION Fluidlink and sheath filter are the only user serviceable parts in this area In line Sheath Filter S...

Страница 33: ...eener PLUS Optical Bench BR Blue Laser Red Laser PMT Module Objective Lens Forward Scatter Detector Flow Cell Focusing Stage 1 of 4 PMT Module Detail BR PMT 1 of 4 Dichroic Mirror 1 of 6 Side Scatter...

Страница 34: ...30 Optical Bench VBR Blue Laser Red Laser PMT Module Objective Lens Forward Scatter Detector Flow Cell Violet Laser Focusing Stage 1 of 6 PMT Module Detail VBR PMT 1 of 6 Band Pass Filter 1 of 7 Dich...

Страница 35: ...S Optical Bench VYB Yellow Laser Violet Laser PMT Module Objective Lens Forward Scatter Detector Flow Cell Blue Laser Focusing Stage 1 of 4 PMT Module Detail VYB PMT 1 of 6 Dichroic Mirror 1 of 6 Side...

Страница 36: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Getting to Know the iQue Screener PLUS 32 THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 37: ...Que Screener PLUS Hardware Manual Part Number 11811 Rev B Operation Operation Articles Daily Startup Daily QC Running a Plate Daily Shutdown iQue Screener PLUS Bottle Fluids iQue Screener PLUS Deck Fl...

Страница 38: ...34 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A iQue Screener PLUS Operation THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 39: ...US Steps 1 Fill the Sheath PLUS ONE and PLUS TWO bottles and empty the Waste bottle Refer to the iQue Screener PLUS Bottle Fluids chapter for detailed instructions 2 Install new deck fluid cartridges...

Страница 40: ...tubing clamp are properly installed as shown in the iQue Screener PLUS Startup Wizard and then click Start The system will go through an initialization and startup procedure that takes approximately 3...

Страница 41: ...et of Validation Beads and checks their performance against a set of standards For each fluorescence channel the mean fluorescence and CV of the brightest peak is checked 1 Prepare Validation Beads by...

Страница 42: ...ics tab and observe the values for the thirteen TEST metrics These are custom calculation metrics that return 1 if the test passes and 0 if the test fails Be sure to scroll through all thirteen test m...

Страница 43: ...e p Step 5 Perform Well Identification p Step 6 Analyze your Data Step 1 Prepare a Plate This example experiment was run using a simple suspension cell line Final cell concentration was 1 000 cells pe...

Страница 44: ...eriment button on the ForeCyt toolbar In the New Experiment dialog choose Blank Experiment Plate Type 96 Well give your experiment a name and click Save Your new blank 96 well experiment will be creat...

Страница 45: ...seconds In the Sample box set the Sip Time to 2 seconds and choose a mapped 96 well plate model that matches the plate you prepared IntelliCyt recommends collecting 1000 2000 events of interest per w...

Страница 46: ...r plate on the deck In the Controller Console window click Run As the plate runs you can monitor the run progress by observing the default scatter plot and looking to the Controller Console for additi...

Страница 47: ...for Well Identification dialog zoom in choose a gating tool and draw a gate around a population that exists in all of your wells Then click Close At this point your wells are calculated What this mea...

Страница 48: ...f the basic techniques in ForeCyt For detailed instructions on ForeCyt data analysis see the video tutorials on the IntelliCyt website A FSC vs SSC scatter plot is used to identify normal and dying ce...

Страница 49: ...the iQue Screener PLUS Steps 1 Optional Long Clean At least once a week and particularly after heavy usage perform a Detector Long Clean a Click Clean in the Controller Console b Choose Detector Long...

Страница 50: ...46 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Operation Daily Shutdown b Follow the instructions in the Shutdown Wizard and click Start to initiate the shutdown...

Страница 51: ...aration SHEATH Hydrodynamic focusing of samples in the flow cell 1 bottle of IntelliCyt Bacteriostatic Con centrate in 1 liter of water Part No 90078 PLUS ONE First phase cleaning solution for cleanin...

Страница 52: ...e filtered distilled water 0 22 m filter or equivalent is recommended Filling the Bottles 1 Release the bottle connectors from the tops of the bottles 2 Remove the bottles from the bottle station 3 Op...

Страница 53: ...TION Touching the bottle station during a run can trigger a false bottle error thereby stopping the run Do not attempt to fill or otherwise touch the bottles while the instrument is running NOTE For l...

Страница 54: ...ill continue to run with warnings but they should be addressed before the next plate is started Do not attempt to address a bottle warning while the system is running Doing so may trigger a false erro...

Страница 55: ...This article describes the fluids found in cartridges or troughs in the iQue Screener PLUS sampling area In the sampling area of the iQue Screener PLUS there are slots for four quarter module troughs...

Страница 56: ...ing after Decon and Cleaner have been run through the line Part No 90285 single Part No 90289 ten HPLC reagent grade filtered distilled water 0 22 m filter or equivalent QSol Buffer IntelliCyt s stand...

Страница 57: ...nstall the cartridge in the appropriate slot Note If there is fluid left over in a cartridge at the end of the day reseal the cartridge with laboratory parafilm and store at 2 8 C for up to a week Usi...

Страница 58: ...54 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A iQue Screener PLUS Operation THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 59: ...r PLUS Hardware Manual Part Number 11811 Rev A Maintenance Maintenance Articles Recommended Maintenance Schedule Standard Cleaning Procedures Changing the Probe and Tubing Fluidics Maintenance Managin...

Страница 60: ...56 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 61: ...Comment Change Probe and Tubing The recommended interval for a probe and tubing change is every 20 hours An automatic warning will display when a probe and tubing change is due Detector Long Clean Per...

Страница 62: ...58 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 63: ...ch one does and when to use it All of the standard cleaning procedures are accessed by clicking the Clean button and using the Clean Wizard Accessing the Clean Wizard To perform any standard cleaning...

Страница 64: ...the Probe and Tubing Clean Duration slider The default is a 20 minute clean consisting of five minutes of Decon five minutes of Cleaner and ten minutes of water Detector Quick Clean This clean is used...

Страница 65: ...to run Detector Debubble This procedure is used to clear air bubbles from the detector fluidic lines and bottles Use if Daily QC shows irregular scatter after fluidics maintenance and when fluids are...

Страница 66: ...62 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 67: ...e is due Required Materials Part No Description 91088 iQue Probe Tubing Assy for iQue Screener PLUS 5 Pk 91087 iQue Probe Tubing Assy for iQue Screener PLUS 10 Pk 91086 iQue Probe Tubing Assy for iQue...

Страница 68: ...e Probe and Tubing Wizard The Change Probe and Tubing Wizard will guide you through the process of changing the probe and tubing To launch the wizard click Device Change Probe and Tubing This will lau...

Страница 69: ...65 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance Changing the Probe and Tubing Wizard Steps 1 Remove Probe and Tubing 2 Prepare Probe and Tubing Assembly...

Страница 70: ...ware Manual Part Number 11811 Rev A Maintenance Changing the Probe and Tubing 66 3 Replace Probe and Tubing NOTE When routing the tubing in the pump head take care not to crimp the tubing in the locki...

Страница 71: ...g Deck Height When the Change Probe and Tubing Wizard completes the Calibrate Deck Height Wizard will automatically launch It is important to recalibrate the deck height after a probe and tubing chang...

Страница 72: ...68 Cleaning the New Tubing When the Deck Height Calibration Wizard finishes you will be prompted to clean the probe and tubing Ensure that the Decon Cleaner and Water troughs cartridges in the fluid s...

Страница 73: ...NE Plus TWO and Sheath Bottle Filters 1 90080 In line Sheath Filter 1 91093 PLUS FluidLink tubing connector 5 Pk 91095 iQue Screener PLUS Maintenance Kit 10 pack 10 91092 Plus ONE Plus TWO and Sheath...

Страница 74: ...Manual Part Number 11811 Rev A Maintenance Fluidics Maintenance 70 When the wizard launches a list of required items is displayed Make sure you have all the supplies that are needed Wizard Steps 1 Em...

Страница 75: ...71 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance Fluidics Maintenance 2 Replace Bottle Filters 3 Replace In line Sheath Filter...

Страница 76: ...Probe and Tubing 5 Replace FluidLink NOTE Take care when threading the new FluidLink into the detector inlet Make sure the tubing is flush with the ferrule Make sure the threads are aligned and the Fl...

Страница 77: ...er PLUS Hardware Manual Part Number 11811 Rev A Maintenance Fluidics Maintenance Correct inner tubing flush with ferrule Incorrect inner tubing protruding Incorrect inner tubing recessed 6 Replace Pro...

Страница 78: ...74 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance 7 Fluidics Check 8 Priming the System...

Страница 79: ...Plate Model Offset for that well Fixed Sampling Offset Deck Height The deck height position is calibrated during instrument installation and re checked prior to each plate This is done by having the c...

Страница 80: ...re is a field for Plate Model This is where the plate model is selected By default the system comes with only two plate models listed the Generic 96 Well Plate and Generic 384 Well Plate models Additi...

Страница 81: ...ging Plate Models Adding a New Plate Model 1 Click Device Manage Plate Models 2 In the Manage Plate Models screen click Add and enter the name manufacturer and type for the new plate model 3 Click Sav...

Страница 82: ...78 4 This will launch the Map and Calibrate Wizard Place an example of the plate you are adding in the gripper and click Next As soon as you click Next the instrument will map twelve or more depth po...

Страница 83: ...is means that the probe tip will go to a location 5 mm above the bottom of the well This is optimal for most assays However there may be situations where sipping higher or lower in the well is require...

Страница 84: ...Set Offset Location step The normal sample position is from the center of the well When using flat bottom plates however the vortexing action of the shaker causes sample to accumulate on the edges of...

Страница 85: ...then use the X and Y arrows to move the probe to an offset position No XY Offset probe samples from center XY Offset probe samples from edge of well 96 Well v bottom plate shown of the well 96 well fl...

Страница 86: ...n the Edit Plate Model window click Make Default 4 A confirmation prompt will appear Click Yes 5 Click Save in the Edit Plate Model screen The Plate Model is now the default and will be used on new Ex...

Страница 87: ...yt software that allows you to interact with the various instrument subcomponents directly This can be helpful when troubleshooting instrument problems Accessing Manual Control Mode To access Manual C...

Страница 88: ...well To find a shaker speed that is safe fill a plate with water at the volume you want to test place it on the deck and use the Shaker Control to turn the shaker on to test various speeds Verify Samp...

Страница 89: ...isolate these subsystems and troubleshoot 1 Click the Water button to move the probe over the Water cartridge trough 2 Click the Z down button to lower the probe into the water 3 Unplug the tubing fr...

Страница 90: ...86 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 91: ...bing If the slugs are not moving at all or if they are moving very erratically you may have a partial or total clog Types of Clogs Probe and Tubing Clog The most common clog exists in the probe and tu...

Страница 92: ...ween 1 3 x106 cells mL Use of higher cell densities may clog the instrument Certain sample types are prone to aggregation so it may be necessary to include small amounts of an additive such a QSol buf...

Страница 93: ...cond Buffer rinse configured as three 1 second rinses In this example we ve created a 3 second rinse after every 6 wells which will add about 50 seconds to a 96 well plate run However it can sometimes...

Страница 94: ...90 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Maintenance THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 95: ...91 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Special Topics Articles Moving an iQue Screener PLUS Connecting iQue Screener PLUS Power and Data Cables Purge and Prime...

Страница 96: ...92 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Special Topics THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 97: ...bottles and troughs 3 Wipe work surfaces down with disinfectant 4 Turn off ForeCyt and shut down the computer 5 Unplug power data cables and fluidics lines Moving the Instrument 1 Due to its weight 4...

Страница 98: ...11811 Rev B Special Topics Moving an iQue Screener PLUS 94 After a Move 1 Reconnect the power and data as described in Connecting iQue Screener PLUS Power and Data Cables 2 Fill all the bottles and t...

Страница 99: ...also any time that the instrument needs to be physically moved to a new location The iQue Screener PLUS Uninterruptible Power Supply Each iQue Screener PLUS comes with an Uninterruptible Power Supply...

Страница 100: ...on the UPS this is not required for safety but having the computer on battery backup can prevent work interruption in the case of a power outage CAUTION The iQue Screener PLUS s UPS is required equipm...

Страница 101: ...97 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Connecting Power and Data Cables 110V Power and Data Connection Diagram 220V Power and Data Connection Diagram...

Страница 102: ...98 iQue Screener PLUS Hardware Manual Part Number 11811 Rev A Special Topics THIS PAGE INTENTIONALLY LEFT BLANK...

Страница 103: ...ove all fluids from the lines This prevents bacterial growth and protects the fittings and lines from freezing Subsequently any iQue Screener PLUS that has been purged needs to be re primed before it...

Страница 104: ...al Part Number 11811 Rev B Special Topics Purge and Prime 100 3 Ensure that you have the required supplies and time to complete the operation as shown in the introduction to the Purge Fluidics Wizard...

Страница 105: ...101 iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 5 Wizard Step 2 Purging System 6 Wizard Step 3 Purge Pressure Sensor Lines...

Страница 106: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 102 7 Wizard Step 4 Reconnect Pressure Sensor Lines and Power Down...

Страница 107: ...ty 500 mL beaker 1 Ensure that instrument power and data are properly connected as shown in Connecting iQue Screener PLUS Power and Data Cables 2 Ensure that the bottles are filled and the bottle stat...

Страница 108: ...iQue Screener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 104 6 Wizard Step 1 Initial Prime 7 Wizard Step 2 Priming the Pressure Sensors...

Страница 109: ...ener PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 8 Wizard Step 3 Reconnect the Pressure Sensor Lines and Install Sheath Filter 9 Wizard Step 4 Connect the FluidLink Pro...

Страница 110: ...ner PLUS Hardware Manual Part Number 11811 Rev B Special Topics Purge and Prime 106 10 Wizard Finish Final Prime After the system has been primed click Power on Detector to start the detector see Dail...

Страница 111: ...IntelliCyt Technical Support IntelliCyt Corporation 9620 San Mateo NE Albuquerque NM 87113 USA www intellicyt com Telephone 505 345 9075 opt 2 E mail support intellicyt com...

Страница 112: ......

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