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ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5

26

b. Electrical shutter operation or action

The electrical shutter operation is used to get a proper signal level when signal overflow
happens due to too much input photons in the normal readout operation. In this operation, the
fastest frame rate is operated at full resolution even when the exposure time is short.

Figure 10-2

(2) In Lightsheet Readout Mode

Figure 10-3

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Содержание ORCA-Lighting C14120-20P

Страница 1: ...onal injury and damage to property when using this camera The manual describes the correct handling method of the camera and provides instructions that should be followed to avoid accidents Read this...

Страница 2: ...Blank page...

Страница 3: ...o the user and even death Improper handling of the camera without observing these cautions could lead to personal injury to the user or damage to property This symbol indicates a cautionary item that...

Страница 4: ...ands Doing so can lead to electric shock Do not attempt to dismantle or modify the camera Doing so can also lead to damage and even injury as some internal components become very hot or high voltage D...

Страница 5: ...m from the surface of the C mount adapter on the camera Doing so can scratch the protective glass Some wide angle lenses in particular can have a thread of 7 mm or more Shipping precautions When trans...

Страница 6: ...Instruction manual CD ROM 1 Options SMA BNC cable A12106 05 SMA SMA cable A12107 05 CoaXPress interface board M9982 30 CoaXPress interface cable 4 cables A14590 03 40 3 m Circulating water cooler C314...

Страница 7: ...ce of magnetism or ratio waves Places where there are vibrations Places where the camera may come into contact with corrosive gases such as chlorine or fluorine Places where there is a lot of dust Do...

Страница 8: ...te procedure of Coaxpress interface board 20 9 ABOUT IMAGE ACQUISITION 21 9 1 READOUT METHOD OF THE IMAGE 21 9 2 PRECAUTIONS WHEN USING THE SENSOR 21 10 CAMERA SETTINGS AND FUNCTIONS 22 10 1 SETTINGS...

Страница 9: ...HOSES 47 12 MAINTENANCE 48 12 1 CARE 48 12 2 WHEN USING AT WATER COOLING 49 13 TROUBLSHOOTING 50 13 1 TURNING ON THE POWER 50 13 2 ALTHOUGH IMAGES ARE TRANSFERRED 50 13 3 AT WATER COOLING 51 14 SPECI...

Страница 10: ...ture Cooling of this equipment is done using a Peltier element With a Peltier element when current is supplied one surface is cooled and the other surface is heated CMOS image sensor is positioned on...

Страница 11: ...is very small Figure 5 1 6 Modes for the image acquisition Camera readout modes Camera operation modes Readout methods This camera has various modes for the image acquisition The modes are set in the...

Страница 12: ...ipment This camera has Trigger output function to be able to control external equipment from the camera Refer to 10 7 TRIGGER OUTPUTS 8 Real time defect pixel correction This camera is correcting the...

Страница 13: ...er input connector c Trigger input connector h WATER connector d Timing out connector 1 2 3 i Fan e STATUS lamp j Air outlet Do not connect anything to a connector not to use When the camera heats up...

Страница 14: ...unch the application software after all the lamps stay on green Because the computer does not recognize the camera All the lamps do not stay on green when the computer CoaXPress interface board is not...

Страница 15: ...er to 11 WHEN USING WATER COOLING METHOD i Fan for Air cooling This is a fan for heat ventilation that blown air inside the camera The fan does not work in Water cooling If the camera is being operate...

Страница 16: ...es When cables are connected turn off the power supply of the camera and peripheral devices Use the above options for the CoaXPress interface board M9982 30 and the CoaXPress interface cables A14590 0...

Страница 17: ...lug the AC supply Then remove the cause of the overheating when it is cooled down 3 Cooling methods The camera has two cooling methods Air cooling method and Water cooling method The default of coolin...

Страница 18: ...adapter attached to the camera Refer to 8 4 2 When the mount part is ready attach the same type of a lens or an optical system When using the C mount do not to screw the lens more than 7 mm from the...

Страница 19: ...is turning Turn on the computer and peripherals Make sure STATUS lamp and CoaXPress interface connector lamps stay on green Launch the application software Camera setting Set the camera setting when t...

Страница 20: ...space where you can work stably and do it without risks When the mount adapter is attached or detached be careful not to make a scratch and not to put dust on the window glass in front of the camera...

Страница 21: ...rew surely 4 Tighten the 2 screws of the C mount by using the hexagonal wrench 5 Attach the C mount lens on the adapter Use a lens with the screw part of less than 7 mm 8 4 2 When using TFL II mount D...

Страница 22: ...I does not match the firmware version of the interface board the following message prompting update of the interface board may be displayed When this message is displayed you need to update the firmwa...

Страница 23: ...e failure in part of the silicon wafer resulting in white spots Currently this phenomenon is not preventable If CMOS image sensor is at a fixed temperature recurrence of the white spot increases propo...

Страница 24: ...area is specified Full resolution readout 10 4 Exposure time setting Set the exposure time when the following modes Free running mode Edge trigger mode Global reset edge trigger mode 8 2 ms at Free ru...

Страница 25: ...mode Global reset Edge trigger mode Level trigger mode Global reset Level trigger mode Synchronous readout trigger mode Start trigger mode Readout methods Normal readout 1x1 Binning readout 2x2 4x4 Su...

Страница 26: ...intained because 4 horizontal lines are readout simultaneously Please refer to 10 10 REFERENCE FRAME RATE CALCULATION METHOD about the frame rate 10 2 2 Lightsheet Readout Mode Lightsheet Readout Mode...

Страница 27: ...n which the exposure time is longer than the one frame readout time and electrical shutter operation in which the exposure time is shorter than the one frame readout time These operations are automati...

Страница 28: ...electrical shutter operation is used to get a proper signal level when signal overflow happens due to too much input photons in the normal readout operation In this operation the fastest frame rate i...

Страница 29: ...laser and camera to control trigger timing In each external trigger mode of the camera the delay can be set to the trigger signal input to the camera by command With this setting a range of trigger c...

Страница 30: ...al reset function enables to reset the electric charge of all pixels at the same time Then all pixels can start exposure at the same time The exposure time is set by application software With this Glo...

Страница 31: ...high or low period of the input trigger pulse The example below is for the trigger level High The exposure of the first four lines 0H 3H begins when the trigger signal becomes High and the exposure of...

Страница 32: ...spinning disk confocal microscope and the camera exposure time is synchronized to the spinning disk s rotation speed it is possible to eliminate uneven illumination called banding noise caused by vari...

Страница 33: ...sing edge b Trigger times Also in the Synchronous readout trigger mode synchronous readout can be controlled by specifying the number of timing pulses to determine the exposure time The following figu...

Страница 34: ...sure the phenomenon after stimulation it is possible to start continuous image acquisition at the stimulation timing Start trigger mode is to start operating the camera by a trigger input for continuo...

Страница 35: ...g readout With this camera 2 2 binning and 4 4 binning readout are available by adding the signal of adjacent pixels in the digital domain Binning readout is a method for achieving high sensitivity in...

Страница 36: ...ity mode 4H 50 432 s 2 In Light Sheet Readout Mode The readout time in the lightsheet mode is adjustable from the calculated value in the normal area mode as the minimum value upto 200 s 10 6 EXPOSURE...

Страница 37: ...output Programmable timing output Trigger ready output Continuous High output positive output Continuous Low output negative output The global exposure timing output is not provided however because th...

Страница 38: ...y time to Read End the end of readout timing or Vsync The setting range for delay time is 0 s to 10 s and the setting range for pulse width is 1 s to 10 s The relation between the parameter which can...

Страница 39: ...nge of pulse width is 1 s to 10 s The relation between the parameter which can be set with each reference signal and an output signal becomes as shown below Reference signal Output signal Read End Cam...

Страница 40: ...e during the exposure period To reduce useless time to be as short as possible it is necessary to know the period when the camera can accept a trigger for the next frame The trigger ready output shows...

Страница 41: ...ting formula Free running mode Exp Vn 4 2 1 Exp 2 x4H Exp Vn 4 2 1 Vn 4 4 x4H External trigger mode Edge trigger mode 1 Exp Vn 4 6 x4H Level trigger mode Synchronous readout trigger mode 1 Exp Vn 4 3...

Страница 42: ...2688 2592 30 30 30 30 2560 2560 30 30 30 30 2048 2048 38 38 38 38 1024 1024 76 75 76 75 512 512 150 146 151 145 256 256 291 279 295 275 128 4 3965 2478 4957 2203 The frame rate in binning mode will be...

Страница 43: ...s using soft water except pure water for cooling water If you plan on using water other than soft water as recommended for example antifreeze etc choose the water which followed notices of 12 2 WHEN U...

Страница 44: ...r to avoid water drop or water splash c Deterioration of hoses Replace the water hose with a new one whenever it cannot keep 0 45 L min flow rate for water circulation due to the hose deterioration 5...

Страница 45: ...a is used on the microscope If it is easy to connect the hose onto the camera after the camera is set onto the microscope then it is OK to connect the hose after the camera is set on the microscope 4...

Страница 46: ...era and the computer Switch the cooling method Turn off the camera Turn on the circulating water cooler Turn on the computer and peripherals Make sure STATUS lamp and CoaXPress interface connector lam...

Страница 47: ...is unnecessary to set the DCAM Configurator whenever the camera is started When the setting is changed restart the camera By restarting the setting is reflected on the camera After cooling method was...

Страница 48: ...splayed Click the Hardware icon and set the cooling method to Water cooling Refer to the instruction manual of DCAM Configurator about how to operate the DCAM Configurator window The manual is stored...

Страница 49: ...er 3 Remove water or water drop inside the hose and the camera by air Blow air from one side of hose Prepare water absorption sheet such as Waste Towel or so and catch pan on another side of hose in o...

Страница 50: ...dow gently 3 Confirm whether dust is not left Attach the camera to an optics and check if there is dust or not under the uniform light condition If there is dust on the image please clean the glass wi...

Страница 51: ...possible to use distilled water or deionized water by conducting periodical check However please notice it increases possibility of corrosion inside cooling water path lowering flow rate or water flow...

Страница 52: ...ange the setting to Air cooling 11 5 13 2 ALTHOUGH IMAGES ARE TRANSFERRED Symptom Cause Measures Chapter Scratches or discoloration visible on the screen Lens is dirty Wipe the lens 12 1 Image is blur...

Страница 53: ...ater Refer to the instruction manual of the circulating water cooler Noise appears on the screen The temperature of cooling water is high more than 25 C Lower the temperature to 25 C The flow of cooli...

Страница 54: ...ode Full well capacity 1 1000 electrons 38 000 electrons Dynamic range 2 650 1 17 000 1 Readout noise 1 2 0 electrons r m s 1 5 electrons median 2 7 electrons r m s 2 2 electrons median Conversion fac...

Страница 55: ...to 129 6 ms Setting range of 4H 12 608 s to 200 s 32 7 ms to 129 6 ms Setting range of 4H 50 432 s to 200 s Minimum readout interval 4608 x 2592 8 3 ms 32 7 ms Readout direction Forward direction b Re...

Страница 56: ...240 V Frequency 50 Hz 60 Hz Typical output DC 18 62 V to DC 19 95 V Max 7 9 A Power consumption Max 200 VA Fluctuations of input power supply voltages are not to exceed 10 of the nominal voltage 4 Op...

Страница 57: ...ORCA Lightning Digital CMOS Camera C14120 20P Instruction manual_Ver 2 5 55 7 Spectral response characteristics Typical Graph 14 1...

Страница 58: ...ORCA Lightning Digital CMOS Camera C14120 20P Instruction manual_Ver 2 5 56 15 DIMENSIONAL OUTLINES 15 1 F MOUNT TYPE Unit mm...

Страница 59: ...ORCA Lightning Digital CMOS Camera C14120 20P Instruction manual_Ver 2 5 57 15 2 C MOUNT TYPE Unit mm...

Страница 60: ...ORCA Lightning Digital CMOS Camera C14120 20P Instruction manual_Ver 2 5 58 15 3 TFL II MOUNT TYPE Unit mm...

Страница 61: ...turing of the camera You may be liable for repairs during the warranty period in the event of a natural disaster or if you handle the camera contrary to the instructions in this manual use it without...

Страница 62: ...sys hpk co jp Local contact information worldwide can be found at www hamamatsu com The contents of this manual are subject to change without notice The unauthorized duplication or distribution of al...

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