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qCMOS Camera C15550-20UP / C15550-20UP01 Instruction manual_Ver.1.3

Contents

SAFETY PRECAUTIONS ................................................................................... 1

SYMBOLS ........................................................................................................................... 1

1-2

CLASSIFICATION OF WARNINGS .................................................................................... 1

CHECK THE CONTENTS OF PACKAGE .......................................................... 6

INSTALLATION .................................................................................................. 7

OVERVIEW ....................................................................................................... 10

FEATURES ....................................................................................................... 10

NAME AND FUNCTION OF PARTS ................................................................ 13

CONNECTION .................................................................................................. 16

WATER COOLING ........................................................................................... 18

8-1

CAUTIONS ........................................................................................................................ 18

8-2

CONNECTION OF WATER COOLING HOSES ............................................................... 20

8-3

DISCONNECTION OF WATER COOLING HOSES ......................................................... 21

OPERATIONS .................................................................................................. 22

9-1

OPERATING PRECAUTIONS .......................................................................................... 22

9-2

PREPARATING FOR IMAGING ........................................................................................ 23

9-3

IMAGING ........................................................................................................................... 23

9-4

END OF IMAGING............................................................................................................. 23

9-5

STARTUP DCAM CONFIGURATOR ................................................................................ 24

9-6

UPDATE PROCEDURE OF COAXPRESS INTERFACE BOARD ................................... 26

10.

DESCRIPTION OF CMOS IMAGE SENSOR ................................................... 27

10-1

THEORY OF CMOS IMAGE SENSOR ............................................................................. 27

10-2

READOUT METHOD OF CMOS IMAGE SENSOR .......................................................... 28

10-3

PRECAUTION WHEN USING CMOS IMAGE SENSOR .................................................. 28

11.

DESCRIPTION OF VARIOUS FUNCTIONS .................................................... 29

11-1

AREA READOUT MODE, PHOTON NUMBER RESOLVING MODE ................................ 29

11-2

LIGHTSHEET READOUT MODE ...................................................................................... 48

11-3

REAL-TIME DEFECT PIXEL CORRECTION ................................................................... 56

11-4

DATA REDUCTION FUNCTIONS ..................................................................................... 57

11-5

MASTER PULSE ............................................................................................................... 58

12.

MAINTENANCE ................................................................................................ 59

Содержание C15550-20UP

Страница 1: ...o avoid personal injury and damage to property when using this camera The manual describes the correct handling method of the camera and provides instructions that should be followed to avoid accident...

Страница 2: ...Blank page...

Страница 3: ...e user and even death Improper handling of the camera without observing these cautions could lead to personal injury to the user or damage to property This symbol indicates a cautionary item that shou...

Страница 4: ...oing so can lead to electric shock Do not attempt to dismantle or modify the camera Doing so can also lead to damage and even injury as some internal components become very hot or high voltage Do not...

Страница 5: ...hanism When used with a high sensitivity long exposure camera such the ORCA Quest it may be possible to detect photons originating from the F mount light leakage In this case it is recommended to crea...

Страница 6: ...CC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiat...

Страница 7: ...n using water other than soft water as recommended for example antifreeze etc refer to description of cooling water which is written in 12 MAINTENANCE or contact a Hamamatsu subsidiary or your local d...

Страница 8: ...P01 Before Use Booklet 1 C15550 20UP C15550 20UP01 Instruction manual CD ROM 1 QC sheet 1 Option Cooling water hose 2 hoses A10788 04 SMA BNC cable A12106 05 SMA SMA cable A12107 05 CoaXPress interfac...

Страница 9: ...gnetism or radio waves Places where there are vibrations Places where the camera may come into contact with corrosive gases such as chlorine or fluorine Places where there is a lot of dust How to plac...

Страница 10: ...ATIONS 22 9 1 OPERATING PRECAUTIONS 22 9 2 PREPARATING FOR IMAGING 23 9 3 IMAGING 23 9 4 END OF IMAGING 23 9 5 STARTUP DCAM CONFIGURATOR 24 9 6 UPDATE PROCEDURE OF COAXPRESS INTERFACE BOARD 26 10 DESC...

Страница 11: ...OUBLESHOOTING 61 13 1 IMAGE IS NOT TRANSFERRED 61 13 2 ALTHOUGH IMAGES ARE TRANSFFERED 61 14 SPECIFICATIONS 62 14 1 CAMERA SPECIFICATIONS 62 14 2 CONDENSATION 65 14 3 SPECTRAL RESPONSE CHARACTERISTICS...

Страница 12: ...nductor technologies and realizes extremely low readout noise 0 27 electrons r m s 2 Photon number resolving capability Photon number resolving is realized within each pixel enabling superior low ligh...

Страница 13: ...xel correction feature to further improve image quality The correction can be performed in real time without sacrificing any of the readout speed 9 Interface This camera has both CoaXPress interface a...

Страница 14: ...modes The camera has three operation modes 1 Free running mode in which the exposure and readout timing are controlled by the internal microprocessor 2 External trigger mode in which the exposure and...

Страница 15: ...era C15550 20UP C15550 20UP01 Instruction manual_Ver 1 3 13 6 NAME AND FUNCTION OF PARTS 1 C15550 20UP C mount type Figure 6 1 2 C15550 20UP F mount type Figure 6 2 a b c d e f g h i k j b c d e f g h...

Страница 16: ...en a connection interface is changed from CoaXPress to USB and vice versa the application software must be closed and the camera must be turned off Do not connect CoaXPress and USB interface simultane...

Страница 17: ...ed to the USB interface connector on the computer When a connection interface is changed from CoaXPress to USB and vice versa the application software must be closed and the camera must be turned off...

Страница 18: ...ONNECTION Refer to the figure when connecting the various cables 1 CoaXPress interface Figure 7 1 2 USB interface Figure 7 2 CoaXPress interface board to connector 1 4 Circuit water cooler a b c Camer...

Страница 19: ...nnection interface is changed from CoaXPress to USB and vice versa the application software must be closed and the camera must be turned off Do not connect CoaXPress and USB interface simultaneously H...

Страница 20: ...ommended for example antifreeze etc refer to description of cooling water which is written in 12 MAINTENANCE or contact a Hamamatsu subsidiary or your local distributor 3 Recommendation temperature Ha...

Страница 21: ...5 mm Figure 8 1 If the hose size on circulating water cooler is the same as blue hose remove gray hose from the joint part The gray hose can be removed when blue hose is pulled with pushing the butto...

Страница 22: ...is used on the microscope If it is easy to connect the hose onto the camera after the camera is set onto the microscope then it is OK to connect the hose after the camera is set on the microscope 4 Co...

Страница 23: ...Remove water or water drop inside the hose and the camera by air Blow air from one side of hose Prepare water absorption sheet such as Waste Towel or so and catch pan on another side of hose in order...

Страница 24: ...be started before start operating the camera in water cooling A fan inside the camera does not rotate See 8 WATER COOLING for instruction of water cooling Do not switch to water cooling method when wa...

Страница 25: ...dark cloth Be careful maintain the clearance distances in the venting areas of the camera for proper cooling air flow 9 2 1 WHEN USING AIR COOLING 1 Connect the equipment as shown in Figure 7 1 befor...

Страница 26: ...ure to startup DCAM Configurator 1 Open Setup exe in the DCAM API software s folder If the DCAM API software is not installed on your computer insert the media of DCAM API software in the slot of your...

Страница 27: ...Even if the camera s power supply is turned off the state of setting is kept The state of setting can confirm according to Hardware icon on DCAM Configurator window After the startup operate DCAM Con...

Страница 28: ...ware version of the interface board the following message prompting update of the interface board may be displayed When this message is displayed you need to update the firmware of the interface board...

Страница 29: ...0 1 The scientific CMOS image sensor used in this camera has an on chip CDS correlated double sampling circuit which plays an important role in achieving low noise In addition column amplifiers and A...

Страница 30: ...esulting in white spots Currently this phenomenon is not preventable If CMOS image sensor is at a fixed temperature recurrence of the white spot increases proportionally with the exposure time so this...

Страница 31: ...dout from camera individually for all pixels 2 Binning readout 2 2 4 4 readout With this camera 2 2 binning readout and 4 4 binning are available by adding the signal of adjacent pixels in the digital...

Страница 32: ...EADOUT SPEED SCAN SPEED The Standard scan readout speed can achieve a frame rate of 120 fps for full resolution with low noise 0 43 electrons r m s and the Ultra quiet scan readout speed can achieve e...

Страница 33: ...44 8 15 432 4 19 841 External trigger mode Edge trigger Level trigger Global reset edge trigger Global reset level trigger 1 Vn2 Exp2 3 1H 4096 2304 119 2048 134 1024 266 512 524 256 1013 8 10 683 4 1...

Страница 34: ...tal output Operation modes Binning Calculation formula Hn Vn Max frame rate fps Free running mode 1 1 If Exp2 13 889 1 Vn5 1H Others 1 Exp2 1H 4096 2304 17 6 2048 2048 39 8 1024 1024 158 512 512 532 2...

Страница 35: ...1 2 2 2048 1152 94 3 4 4 1024 576 120 External trigger mode Edge trigger Level trigger Global reset edge trigger Global reset level trigger 1 1 If Vn2 Exp2 2 V4 1 Vn4 1 1H Others 1 Vn2 Exp2 3 1H 4096...

Страница 36: ...p2 2 V4 1 Vn4 1 1H Others 1 Vn2 Exp2 3 1H 4096 2304 35 3 2048 2048 79 5 1024 1024 266 512 512 524 256 256 1013 256 8 10 683 256 4 12 626 2 2 2048 1152 119 4 4 1024 576 119 External trigger mode Synchr...

Страница 37: ...00 2048 5 62 1024 11 1 512 22 1 256 43 5 8 643 4 826 External trigger mode Edge trigger Level trigger 1 Vn2 Exp2 3 1H 4096 2304 2 49 2048 2 80 1024 5 58 512 11 0 256 21 5 8 275 4 340 External trigger...

Страница 38: ...nal trigger mode Edge trigger Level trigger Vn2 1H to 1800 s External trigger mode Global reset edge trigger Global reset level trigger 172 8 s to 1800 s External trigger mode Synchronous readout trig...

Страница 39: ...out The normal readout mode is suitable for observation monitoring field of view and focus adjustment and animation because it can operate the fastest frame rate with full resolution In case of standa...

Страница 40: ...a proper signal level when signal overflow happens due to too much input photons in normal readout mode In this mode the fastest frame rate is 120 fps Standard scan via CoaXPress 17 6 fps standard sca...

Страница 41: ...the timing information 1 Edge trigger mode The Edge trigger mode is used so that the exposure starts according to an external signal Exposure time is set In this mode the exposure of the first line b...

Страница 42: ...ic charge of all pixels at the same time Then all pixels can start exposure at the same time With this Global reset Edge trigger mode the exposure of all pixels begins on the edge rising falling timin...

Страница 43: ...The example below is for the trigger level High The exposure of the first and second lines begin when the trigger signal becomes High and the exposure of the third and fourth lines begin after the rea...

Страница 44: ...charge of all pixels at the same time Then all pixels can start exposure at the same time The example below is for the trigger level High With this Global reset Level trigger mode the exposure of all...

Страница 45: ...l for securing as long exposure time as possible while controlling the exposure start timings by external trigger signals Normal operation when the Trigger Time is set as 1 The Synchronous readout tri...

Страница 46: ...can be controlled by specifying the number of timing pulses to determine the exposure time The input trigger is valid only during the trigger ready is enabled The following figure shows the exposure...

Страница 47: ...rks at the highest frame rate because it is operated in internal trigger mode In Start trigger mode the camera starts exposure and switches to internal trigger mode by the edge of an external trigger...

Страница 48: ...same time There is no output signal when the exposure time is less than the frame rate 2 Any row exposure timing output Global exposure timing output shows the global exposure timing where all lines e...

Страница 49: ...amera is working in the Edge trigger mode the next frame can start after the previous frame exposure is done Thus the camera cannot accept a trigger for the next frame during the exposure period To re...

Страница 50: ...rom the top to the bottom line Figure 11 13 or from the bottom to the top line Figure 11 14 in Lightsheet Readout Mode Figure 11 13 Normal area mode Figure 11 14 Lightsheet Readout Mode 11 2 2 ABOUT R...

Страница 51: ...frame rate fps Free running mode 1 Vn2 Exp2 2 1H 4096 2304 119 2048 134 1024 267 512 526 256 1021 8 11 574 4 13 888 External trigger mode Edge trigger 1 Vn2 Exp2 3 1H 4096 2304 119 2048 134 1024 266...

Страница 52: ...Exp2 Vn2 2 1H 4096 2304 17 6 2048 2048 39 8 1024 1024 158 512 512 526 256 256 1021 256 8 11 574 256 4 13 888 External trigger mode Edge trigger If Exp2 Vn2 2 Vn3 1 Vn3 1 1H Others 1 Exp2 Vn2 3 1H 409...

Страница 53: ...024 267 512 512 526 256 256 1021 256 8 11 574 256 4 13 888 The frame rate value is valid 3 digits and rounded down to 4th digit The calculation formula and the frame rate value of Start trigger mode E...

Страница 54: ...manual_Ver 1 3 52 11 2 5 TIMING DIAGRAM 1 Free running mode Figure 11 15 Ex Top to bottom readout 2 Edge trigger mode External trigger mode Figure 11 16 Ex rising edge Top to bottom readout Delay Jitt...

Страница 55: ...qCMOS Camera C15550 20UP C15550 20UP01 Instruction manual_Ver 1 3 53 3 Start trigger mode Figure 11 17 Ex rising edge Top to bottom readout Delay Jitter 4H Maximum 1H Standard scan 1H 7 2 s to 237 6 s...

Страница 56: ...Programmable timing output By using the programmable timing output synchronizing with external devices is simple A system which needs simple timing signal does not require a delay unit or pulse genera...

Страница 57: ...you choose Hsync for the reference of programmable timing output camera can output some pulses before start the exposure It is called as Pre Hsync You can set the number of Pre Hsync 3 Trigger ready o...

Страница 58: ...real time pixel correction features to improve image quality The correction is performed in real time without sacrificing the readout speed at all This function can be turned ON and OFF Default is ON...

Страница 59: ...put And with 8 bit digital output the amount of data can be the half of original 16 bit data and each pixel have 256 steps of gradation When you use USB interface the frame rate can be faster with 12...

Страница 60: ...lt is OFF The master pulse supports free running mode start trigger mode and burst mode The range of interval time is 5 s to 10 s and the step is 1 s for the master pulse 1 Free running mode The camer...

Страница 61: ...wipe over center area of the window gently 3 Confirm whether dust is not left Attach the camera to an optics and check if there is dust or not under the uniform light condition If there is dust on th...

Страница 62: ...n room it is possible to use distilled water or deionized water by conducting periodical check However notice it increases possibility of corrosion inside cooling water path lowering flow rate or wate...

Страница 63: ...ause Measures Chapter Scratches or discoloration visible on the screen Lens is dirty Wipe the lens 12 Image is blurred Lens is not focused Contact a Hamamatsu subsidiary or your local distributor 17 C...

Страница 64: ...ll well capacity 1 7000 electrons Dynamic range 1 25 900 1 2 Dark current 1 Cooling temperature 20 C 0 016 electrons pixel s Cooling temperature 35 C 0 006 electrons pixel s Dark offset Binning OFF 20...

Страница 65: ...tput Global exposure timing output Any row exposure timing output Trigger ready output 3 programmable timing outputs High output Low output Trigger output level 3 3 V LVCMOS Trigger output polarity Ne...

Страница 66: ...ded down to 4th digit 2 When using frame bundle function on DCAM API 3 Mechanical specifications Cooling method Forced air cooled Water cooled Cooling temperature Forced air cooled Ambient temperature...

Страница 67: ...tively Refer to 15 DIMENSIONAL OUTLINES for detail of dimensions 7 Applicable standards EMC EN61326 1 2013 Class A FCC 47 CFR FCC Part15 Subpart B Class A 14 2 CONDENSATION At the Water cooling if amb...

Страница 68: ...qCMOS Camera C15550 20UP C15550 20UP01 Instruction manual_Ver 1 3 66 14 3 SPECTRAL RESPONSE CHARACTERISTICS TYP Graph 14 2...

Страница 69: ...qCMOS Camera C15550 20UP C15550 20UP01 Instruction manual_Ver 1 3 67 15 DIMENSIONAL OUTLINES 15 1 C15550 20UP Unit mm...

Страница 70: ...qCMOS Camera C15550 20UP C15550 20UP01 Instruction manual_Ver 1 3 68 15 2 C15550 20UP01 Unit mm...

Страница 71: ...r repairs during the warranty period in the event of a natural disaster or if you handle the camera contrary to the instructions in this manual use it without due caution or try to modify it 3 We will...

Страница 72: ...hpk co jp Local contact information worldwide can be found at www hamamatsu com The contents of this manual are subject to change without notice The unauthorized duplication or distribution of all or...

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