90
Advanced Operations
6.5 Multi-Wavelength mode – measurements at more than one wavelength
In the multi-wavelength mode, absorbance values can be
measured at up to four wavelengths and the results can be
mathematically processed to obtain sums, differences and
relationships.
Absorbance measures the amount of light absorbed by the sample,
in units of absorbance.
% Transmittance measures the percent of the original light that
passes through the sample and reaches the detector.
Turning on the concentration factor allows selection of a specific
multiplier for converting absorbance readings to concentration. In a
graph of concentration versus the absorbance, the concentration
factor is the slope of the line. Concentration is calculated using a
single factor for each wavelength, which is input by the user.
6.5.1 Set the reading mode at different wavelengths
Press
Multi Wavelength
in the Main Menu. Press
Options
for
Parameter Setup.
Table 13 Multi-wavelength setup options
Options
Description
More
For further Options
Store Off/On
With the
Store On
setting, all measurement data are stored automatically. With the
Store Off
setting, no measurement data are stored.
% Trans/Abs
To
switch to % transmittance, concentration or absorbance readings
λ
Wavelength
To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement
wavelength. The entered wavelength must be in the range from 340–900 nm.
Timer icon
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement
program.
Concentration Factor
Multiplication factor for converting absorbance values into concentration values.
Concentration
Resolution
To select the position of the decimal point in the calculated concentration readings.
Absorbance Formula
Calculation basis for evaluating samples
Save as User Program
To store the selected parameters as a User Program, see
section 6.1 on page 61
Содержание Portable Spectrophotometer DR 2800
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