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1. Shake 3 L of distilled water in a partly filled container for one
minute to saturate the water with oxygen.
2. Add the contents of one BOD Nutrient Buffer Pillow (Cat. No.
14861-98) for 3 L, and invert several times to mix.
3. Snap the neck off a Voluette Ampule Standard for BOD
(Cat. No. 14866-10) and pipet 7 mL of standard into a
sample bottle.
4. Add 133 mL of the nutrient buffer solution, prepared in step 2,
and 15 mL seed. A 10% by volume of seed in solution will
result (see section 4.3 Sample Seeding).
5. Follow the general procedure for the BOD test using the
0-350 mg/L BOD range and a 5-day test period.
6. Also perform a full strength BOD test on the pure seed
to determine its BOD while determining the BOD of
the standard. Follow the same procedure used to determine
the BOD of a sample. See the iconed procedure, section 3.1
BODTrak Test Procedure for details.
7. To determine the BOD result, see section 4.3.2 Determining
BOD of Sample. The corrected BOD of the standard solution
should be 198 ±30.5 mg/L.
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