CRF-1 Operating Manual
Page 45 of 60
33801-V4 DMN S90
8.2. Oocyte Cryopreservation
Profile name: Oocyte
Applicable models: H00, H01, H03, H07, H08
Start temperature
20°C
Cool
from 20°C to -8°C at 2°C min
-1
Hold for nucleation
at -8°C for 10 minutes
Cool
from -8°C to -30°C at 0.3°C min
-1
Cool
from -30°C to -33°C at 3°C min
-1
Cool
from -33°C to -39°C at 6°C min
-1
Cool
from -39°C to -100°C at 10°C min
-1
Hold
at -100°C
Transfer to storage
Figure 48: Pre-programmed oocyte freezing cycle
8.3. Blastocyst Cryopreservation
Profile name: Blasto
Applicable models: H00, H01, H03, H07, H08
Start temperature
20°C
Cool
from 20°C to -7°C at 1°C min
-1
Hold for nucleation
at -7°C for 10 minutes
Cool
from -7°C to -30°C at 0.3°C min
-1
Hold
at -30°C
Transfer rapidly into liquid nitrogen
Figure 49: Pre-programmed blastocyst freezing cycle
-110
-100
-90
-80
-70
-60
-50
-40
-30
-20
-10
0
10
20
30
0
10
20
30
40
50
60
70
80
90 100 110 120 130 140 150 160 170 180
Time (mins)
Temperature (
degC)
-110
-100
-90
-80
-70
-60
-50
-40
-30
-20
-10
0
10
20
30
0
10
20
30
40
50
60
70
80
90 100 110 120 130 140 150 160 170 180
Time (mins)
Temperature (
degC)
Содержание CRF-1 H00
Страница 1: ...Controlled Rate Freezer CRF 1 Series Operating Manual...
Страница 59: ......
