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CRF-1 Operating Manual

Page 6 of 60

33801-V4 DMN S90

1. Introduction

1.1. Product Description

The Grant CRF-1 series is a unique liquid nitrogen-free controlled rate freezer for biological
material preservation. It is pre-loaded with configuration specific freezing programs suitable
for the freezing of embryos, oocytes, blastocysts, stem cells and spermatozoa.

The CRF-1 is not a Medical Device as defined by European Directive 2007/47/EC.

1.2. Product Accessories

The following optional extras may also be supplied with the CRF-1 series freezer:

CRF-1 CRYOPEN

Cryopen nucleating tool

CRF-1 CARTRIDGE

Cryopen spare cartridges

Brief operating instructions for these product accessories are given in Appendix 2. Please
refer to accessory manuals for full operating instructions.

2. How to use this Operating Manual

This operating manual will allow you to unpack, correctly set-up and operate the CRF-1 series
controlled rate freezer both efficiently and safely. Important safety information is listed in the
following section. The technical specification is included in section 4.

If there is a technical matter that this operating manual does not address or any other
question concerning the CRF-1 product please do not hesitate to contact Grant Instruments
(Cambridge) Ltd.

The general details of your warranty for the CRF-1 series freezer are detailed in section 11.

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Содержание CRF-1 H00

Страница 1: ...Controlled Rate Freezer CRF 1 Series Operating Manual...

Страница 2: ...report 19 6 4 2 Running a TSV log file conversion 20 6 4 3 Generating an event log report 21 6 5 Editing and creating profiles 21 6 5 1 Editing an existing profile 22 6 5 2 Setting the parameters of...

Страница 3: ...8 5 Spermatozoa Cryopreservation Linear Cooling 46 8 6 Oocyte Non Linear Cooling Cryopreservation 47 8 7 Cryovial Cryopreservation 48 8 8 Test and Calibration profiles 48 9 Scientific References 49 1...

Страница 4: ...re Connect 16 Figure 16 Software Status bar 17 Figure 17 Software Choosing Profile 18 Figure 18 Software Generating PDF Reports 20 Figure 19 Software Generating TSV Report 20 Figure 20 Software Profil...

Страница 5: ...49 Pre programmed blastocyst freezing cycle 45 Figure 50 Pre programmed embryonic stem cell freezing cycle 46 Figure 51 Pre programmed sperm linear freezing cycle 46 Figure 52 Pre programmed oocyte n...

Страница 6: ...ol CRF 1 CARTRIDGE Cryopen spare cartridges Brief operating instructions for these product accessories are given in Appendix 2 Please refer to accessory manuals for full operating instructions 2 How t...

Страница 7: ...nsport or storage in humid conditions allow the unit to dry out before connecting it to the supply voltage Only connect the unit to the mains supply using the power supply unit and cables provided Ens...

Страница 8: ...ryobank 96 2D tube system 374084 FluidX data matrix tube 96 65 52319 CRF 1 H06 Plate for 55 1 8ml cryovials Max working volume per vial is 1 0 ml NUNC cryovials 363401 round NUNC cryovials 377267 star...

Страница 9: ...place hands underneath the unit and do not move by pressing on the casework 5 2 Freezer Position Ensure the freezer is positioned on a flat stable surface so that the vents and the air outlet at the...

Страница 10: ...irectly from the mains supply To ensure run data is not lost check the power settings do not allow the laptop to go into standby or hibernation mode Do not close the laptop during a data run as this c...

Страница 11: ...7 or Windows 10 In addition to loading the Grant CRF software you will also be loading the drivers for the USB to serial converter FTDI In the case of Windows 7 only you will be loading Microsoft NET...

Страница 12: ...control Select Yes Figure 5 Software NET Framework Installation User Account Control Installation of the NET framework takes several minutes To complete the installation of NET your computer will rest...

Страница 13: ...xtract the FTDI drivers These are required for the USB to serial converter that you will use to make the hardware connection to the CFR Select Extract Then on the following Device Driver Installation...

Страница 14: ...ing Grant CRF Software from the program list in the start menu on your computer Figure 11 Desktop shortcut icon When the software is run for the first time the default User ID is Admin and the default...

Страница 15: ...or changing towards the Set Temp o C While the CRF 1 is changing it is not possible to run a temperature profile and the Start button and Profile Details are disabled When the value in the Plate Temp...

Страница 16: ...er will also be displayed If the software fails to connect to the unit see Appendix A1 3 for help Figure 15 Software Connect The freezer unit will remain connected until the Disconnect button is press...

Страница 17: ...MN S90 Figure 16 Software Status bar The software suite can now be used to control the freezing cycle as well as configure the freezer edit and create new freezing cycles as well as generate reports w...

Страница 18: ...the chosen profile can be viewed by clicking on the View Details box This will help ensure you have chosen the correct profile before it is run 6 3 1 2 Setting the log file parameters Set the Log Inte...

Страница 19: ...on and are suitable for validation and archiving purposes Four reports can be produced from the encrypted log file using the Report page PDF Detailed Report Includes a graph of the actual and set temp...

Страница 20: ...e location using the dialog box which appears see Figure 19 Note The TSV will not appear in the viewing area Figure 19 Software Generating TSV Report 6 4 2 Running a TSV log file conversion Click GEF...

Страница 21: ...etails 6 5 Editing and creating profiles The CRF 1 is configured with a standard set of profiles specific to the model type but the software provides a great degree of flexibility in programming new p...

Страница 22: ...ofiles Care and consideration should be taken before changing this parameter Click Change Start Temp to change the universal profile start temperature Update the Start Temp box with the required value...

Страница 23: ...ers can be easily met The maximum or minimum parameter values for a segment represent the highest or lowest value that can normally be achieved Any profile created or modified should be tested to conf...

Страница 24: ...only Click once in the Target Temp o C box of the segment to be changed The background will change from white to light blue to indicate it has been selected Type in the new desired target temperature...

Страница 25: ...it please refer to the Troubleshooting section of the CRF 1 Operating Instructions for more information 6 5 2 7 Setting the segment FF offset This value is predefined and should not need to be altered...

Страница 26: ...for any segment type 6 5 2 10 Setting the audible alarm Click once in the Audible Alarm box of the segment to be changed The Alarm Editor dialog box will appear Choose whether to set the alarm to sou...

Страница 27: ...ntly the previous profile stored Care and consideration should be taken before loading profiles as information can be lost Click Load Profile To CRF 1 The Open dialog box opens in the default profiles...

Страница 28: ...ent window to restore a backup of all of the profiles Select the backup to restore from the list in the Open dialog box and click Open The Restore Profile Selection dialog box opens see Figure 29 Tick...

Страница 29: ...ing and configuring reports The reports created are based on a standard template that can be customised via the Report Designer page 6 6 1 User defined report header The Report Template has two header...

Страница 30: ...g Manual Page 30 of 60 33801 V4 DMN S90 Figure 30 Software User Defined Report Header These user defined conditions will appear at the top of a generated report see Figure 31 Figure 31 Software User D...

Страница 31: ...d to suit your file set up and can be a network share Click on the Browse For Folder button next to the Default Dir option to choose a new location see Figure 32 Figure 32 Software Log File Configurat...

Страница 32: ...e ensure that the Include Text box in the Log window on the Software Config page is checked see Figure 34 When the Include Text box is checked a User Text box next to the Log Filename box on the Monit...

Страница 33: ...e default storage folder for saved profiles This location can be varied to suit your file set up and can be a network share Click on the Browse For Folder button next to the Default Dir box to choose...

Страница 34: ...be created when logged on as Admin or another administrator level user Click Users to open the Users page The first user in the list will be highlighted in blue and the User Details for that user will...

Страница 35: ...required information and click Update Be sure not to edit the Administrator s User Level If there is no user with Administrator permissions it will be necessary to reinstall the software to make signi...

Страница 36: ...to prepare and use it Alternative tools for nucleating e g forceps may be used if sterility of the operating environment is not essential 7 3 Sample Preparation Before preparing samples remove the li...

Страница 37: ...such that the samples are in the centre of the freezing plate the straw plug should overhang the freezing plate if required Figure 37 0 25ml IMV straws positioned on sample plate 7 3 1 3 CRF 1 H02 1 8...

Страница 38: ...ample plate Figure 39 SBS microplate positioned on sample plate Once in position place the lid over the sample plate checking that it is located correctly 7 3 1 6 CRF 1 H06 1 8ml Cryovials 1 0ml max f...

Страница 39: ...ture of the plate is logged automatically in an encrypted format The data is stored in the file location shown on the Monitor panel At the end of the cycle the data can be used to create a report or a...

Страница 40: ...on plateau temperature the time displayed changes to Nucleate The freezer unit buzzer sounds and the yellow Nucleate light on the freezer unit illuminates Nucleation should be done at this time The pr...

Страница 41: ...ne second Figure 43 Figure 44 Figure 45 Repeat with the remaining samples and replace the lid of the freezer Note that other methods of ice nucleation may also be employed dependent on experimental pr...

Страница 42: ...data will continue to be logged 7 7 Stopping the Cycle and Generating a Report When all of the vessels have been transferred click on the Stop Cycle button on the software Monitor panel Temperature lo...

Страница 43: ...d stabilised at the start temperature the Cryocooler Status will change to Cycle Ready The Start Cycle button will no longer be greyed out and more samples can be loaded in preparation for another fre...

Страница 44: ...20 C Cool from 20 C to 7 C at 2 C min 1 Hold for nucleation at 7 C for 10 minutes Cool from 7 C to 30 C at 0 3 C min 1 Cool from 30 C to 33 C at 3 C min 1 Cool from 33 C to 39 C at 6 C min 1 Cool fro...

Страница 45: ...rogrammed oocyte freezing cycle 8 3 Blastocyst Cryopreservation Profile name Blasto Applicable models H00 H01 H03 H07 H08 Start temperature 20 C Cool from 20 C to 7 C at 1 C min 1 Hold for nucleation...

Страница 46: ...tozoa Cryopreservation Linear Cooling Profile name Sperm Applicable models H00 H01 H03 H07 H08 Start temperature 20 C Cool from 20 C to 7 C at 2 C min 1 Hold for nucleation at 7 C for 10 minutes Cool...

Страница 47: ...l from 18 1 C to 22 2 C at 0 4 C min 1 Cool from 22 2 C to 25 3 C at 0 3 C min 1 Cool from 25 3 C to 27 8 C at 0 2 C min 1 Cool from 27 8 C to 31 C at 0 1 C min 1 Cool from 31 C to 34 C at 3 C min 1 C...

Страница 48: ...ure 53 Pre programmed cryovial freezing cycle 8 8 Test and Calibration profiles Profile name TCal TP1 TP2 Applicable models H00 H01 H03 H07 H08 Profile name TP3 TP4 Applicable models H02 H05 H06 These...

Страница 49: ...gen and laminin on the cryopreservation of human embryonic stem cells Stem Cells 22 950 961 Lasalle P Testart J and Renard JP 1985 Human embryo features that influence the success of cryopreservation...

Страница 50: ...spilt onto the equipment disconnect it from the power supply and have it checked by a competent person It is the user s responsibility to carry out appropriate decontamination if hazardous material i...

Страница 51: ...ons 2006 We are a member of the B2B compliance scheme Scheme Approval Number WEE MP3338PT SCH which handle our WEEE obligations on our behalf Grant Instruments have been issued with a unique registrat...

Страница 52: ...power lead is not properly connected to the unit Check the power lead is properly connected into the rear of the unit There is no power to the power lead Check the power lead is plugged into a socket...

Страница 53: ...mples are dry before freezing Section 7 3 Samples can be removable with careful use of tweezers Appendix 2 Cryopen Nucleating Tool Operation Please read the instruction manual supplied with the cryope...

Страница 54: ...used Follow the instructions below Take the mini CD supplied with the converter and insert it into the CD DVD drive of your PC Follow the on screen instructions Insert the converter into a USB socket...

Страница 55: ...ng Manual Page 55 of 60 33801 V4 DMN S90 This should be used when setting the COM port in the Software Config panel of the User Software Suite See Figure 55 Figure 55 Configuring the Com Port on the U...

Страница 56: ...a finger into the top hole and pulling it gently but firmly upwards 2 Once the top foam is removed the unit can be gripped by the bottom foam hand hold and with one hand on the sample plate 3 Grasp t...

Страница 57: ...nit on the foam with the cut corners of the foam facing forwards as shown 3 Tighten the strap until it is taut Place the buckle as shown Avoid the buckle touching the unit 4 Fit top foam piece as show...

Страница 58: ...GE AND MAY VOID THE PRODUCT WARRANTY 1 Place unit carefully on a secure surface with the switch and keypad facing upwards to remove the transit bolt 2 Attach blanking plate supplied re using black thu...

Страница 59: ......

Страница 60: ...opean Union Shepreth Grant Instruments Europe B V Cambridgeshire Strawinskylaan 411 SG8 6GB WTC Tower A 4th Floor UK 1077 XX AMSTERDAM THE NETHERLANDS Tel 44 0 1763 260811 salesdesk grantinstruments c...

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