11 Calibration Solutions for Osmometry
11.5 Storage and Shelf Life
When stored unopened at 5°C to 45°C, the calibration and reference materials have a shelf life up to
the expiration date indicated on the packaging.
Do not use the calibration standard after its expiration date!
Once the ampoule has been opened, the calibration and reference material shelf life is:
up to ½ hour at + 22°C.
A strong variance in calibration readings (increasing osmolality) is an indication that the shelf life has
elapsed.
11.6 Use of Other Calibration Solutions
Osmolality
Sodium chloride concentration
Freezing point depression
mOsmol/kg H
Table 5
NaCl solutions for osmometry
(*) Based on Geigy Scientific Tables 8
th
edition (congruent to USPC: usp29nf24s0_c785)
(**) Measured with Gonotec Freezing point osmometer OSMOMAT 030-3P
2
O g
NaCl/kg
H O
in C°
2
100*
3,088
0,186
200*
6,260
0,372
300*
9,463
0,558
400*
12,684
0,744
500*
15,916
0,930
600*
19,147
1,116
700*
22,380
1,302
850**
27,178
1,579
1200**
38,370
2,230
1800**
57,560
3,350
2000**
63,960
3,716
2500**
79,970
4,650
Calibration Solutions for Osmometry
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