5 Calibration of the OSMOMAT auto
5 Calibration of the OSMOMAT auto
Before the total osmolality of sample solutions can be measured, the OSMOMAT auto must be
calibrated with distilled water and a calibration standard.
5.1
Calibration at Two Points
Two clean, dry measuring vessels are first filled via a pipette with 50 µl distilled water and 50 µl
Gonotec calibration standard 300 mOsmol/kg. In this process
there must be no visible air bubbles in the liquid!
The sample holder is numbered 0 through 20 from left to right. The sample holder is to be filled as
follows:
Positions
0
and
1
with
distilled water
Positions
2
and
3
with
300 mOsmol/kg
Use the proper sequence when filling the sample holder. Incorrect placement results in
incorrect calibration!
The sample cover is then placed on the sample holder to prevent premature evaporation of the
samples.
The sample cover reduces the risk of premature evaporation of the samples. However,
it does not protect the samples from leakage.
To perform the calibration, select >
sample
>
calibration
in the initial display. Select >
number of calib-
samples
>
2
in the display to calibrate at two points. Use
choose
,
to select the calibration value of the
calibration standard to be used >
100 200 300 500 600 700 850 900 1200 1500 1800 2000 2500
. The
value for the distilled water is predefined at 0 mOsmol/kg. Confirm your selection with
OK
. The
following display >
sample sequence
again shows your calibration settings and the corresponding
positions of the solutions in the sample holder – e.g.
0 0 0.300 0.300
for a calibration at two points with
distilled water and Gonotec calibration standard 300 mOsmol/kg. Select
OK
to accept the settings.
With
Cancel
you can discard the settings and return to the display >
calibration
.
18 |
Calibration of the OSMOMAT auto
