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FineLINE 35
oligo
column
Operating Instructions
28-9649-57
AA
33
Troubleshooting 6
6 Troubleshooting
If an increased back-pressure is observed, locate the blockage point in the system by
systematically isolating the different components in the line of flow. If the back-pressure
increase is due to a blockage in the column, the following procedures should be
employed.
Problem
Check / possible causes
High back pressure
Column filters blocked, if so, clean or replace.
Refer to
Section 5.2 Cleaning‚ on page 26
.
Equipment before or after the column generates back
pressure.
Connected tubing with too small diameter or bent.
Pressure indicator faulty.
Column leakage
Solvents used not compatible with O-rings and/or
gaskets. Refer to
Section 7.2 Chemical resistance‚ on
.
Connections to and from the pump not correct (loose or
misplaced).
Column not connected correctly.
O-rings not positioned correctly.
Содержание FineLINE 35 oligo
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