5
Close the top mobile phase inlet/outlet.
6
Place a syringe (without plunger) into the hydraulic chamber vent valve, and open
the vent valve.
7
Connect the pump to the hydraulic chamber inlet valve.
8
Pump hydraulic liquid into the hydraulic chamber using appropriate flow rates (see
Section 3.6 Packing flow rates, on page 29).
9
Close the hydraulic vent valve when no air remains in the hydraulic chamber, i.e.
liquid has been forced into the syringe and no more air bubbles come. Immediately
open the bottom mobile phase inlet/outlet. The adapter starts moving down and the
medium starts to consolidate.
10 Note when the adapter comes into contact with the consolidated bed.
There are different scales for stainless steel and plastic bed supports on
the column.
Note:
11 Continue to compress the bed until the target bed height is reached. Calculate the
achieved Packing Factor (PF).
or,
Continue to compress until the target Packing Factor (PF) is reached.
PF=
consolidated bed height
packed bed height
Read the bed height. Calculate the achieved Packing Factor (PF).
12 Turn off the pump when the target bed height is reached.
13 Open the top mobile phase inlet/outlet.
14 Run the column upflow with mobile phase for a few minutes to remove residual air
possibly trapped in the adapter.
The column is now packed. Before using it, evaluation of the packing is
recommended.
Note:
15 It is recommended to replace the tubing with a stop plug [101] at the check valve
[116].
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
99
5 Operation
5.3 Manual packing of the column
