Priming the column
NOTICE
Ensure that the tubing is of sufficient length to be able to reach the
top mobile phase inlet/outlet. Tubing kits are available as acces-
sories.
These instructions apply only to columns with stainless steel bed supports. Instructions
for columns with plastic bed supports are given in the Addendum to the Operating In-
structions, Wetting instructions for plastic bed supports.
Make sure that the column is placed close to the system before priming the
column so that it can be tilted/rotated/emptied without disconnecting the
bottom tubing.
Note:
1
Make sure that the column has been cleaned and prepared for a run (see Cleaning,
on page 56).
2
Make sure that the pressure alarm is active.
3
Pump packing liquid at high flow velocity upflow into the column using a manual
command.
4
Maintain the flow until the bed support in the bottom plate is covered and stops
producing air bubbles. Start and stop the flow several times. Gently tap the column
and shake the tubing while flowing packing liquid into the column to help free the
air bubbles. Drain the column through the bottom phase inlet until 1 cm of liquid
remains at the bottom of the column. Repeat the procedure once.
5
Hold the adapter with the bed support facing upwards and pump packing liquid at
high flow velocities through the top mobile phase inlet/outlet and out through the
bed support until no air bubbles emerge. Start and stop the flow several times. Shake
the tubing while flowing packing liquid into the column to help free air bubbles.
For ÄKTApilot, both the A and B pumps can be used to increase flow velocity.
6
Place the adapter (bed support downwards) into a container of liquid to prevent air
coming into the bed support, adapter and tubing.
The adapter of the AxiChrom 50 column may need extra support to stand
in the container of liquid, for example by leaning it against the wall or an-
other surface.
Note:
7
Stop the flow.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
73
5 Operation
5.1 Intelligent Packing of the column with ÄKTAexplorer and ÄKTApilot
