Action
Comment
Problem
•
Place a centering tool into the mouth
of the column to check the “centering”
of the column tube. For correct position
of the centering tool see Figure 6.26.
•
If the centering tool cannot be fitted into
the mouth of the column properly, fol-
low the instructions under Servicing the
column tube assembly, on page 136.
Difficulties to enter the
adapter into the
column.
•
Tighten the adapter to the adapter rod
using a wrench and a hook wrench key
(for the 100 column only: use another
wrench instead of the hook wrench key)
(Servicing the adapter rod, adapter tub-
ing and top plate (stainless steel bed
support), on page 119). The parts can
loosen after using the column several
times. Check that the adapter rod is
tight before performing each packing
procedure.
The adapter is not tight
enough.
The adapter is partially
unscrewed from the
adapter rod.
•
Prime the adapter at a higher flow ve-
locity. Spray 20% (v/v) ethanol on the
bed support to lower the surface ten-
sion and thereby facilitate air removal.
The adapter was not
thoroughly primed with
buffer.
Large air bubbles
under the adapter bed
support.
•
If large quantities of air are trapped,
restart the packing procedure.
•
Remount the scraper under water.
Air trapped between the
scraper seal and the O-ring is
pushed out due to
compression of the O-ring
when the adapter is lowered.
AxiChrom 50, 70 and 100 columns Operating instructions 28-9331-08 AG
151
7 Troubleshooting
7.2 Adapter
