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Fractionation in practice

When fractionation is complete or if you re-use outlet positions, empty the outlet tubing
by releasing the SNAP connectors on the outlets and allowing the tubing contents to
drain into the fraction containers.

For unattended fractionation, the maximum number of fractions that can be collected
is 14 (using five installed outlet valves). Plan your fractionation settings carefully to avoid
creating more than the maximum number of fractions. Use Conditional Fractionation
and Peak fractionation to optimize the number of fractions.

If the number of fractions exceeds the number of available outlets, it is possible to re-
use outlet positions for fractionation in different phases (for example, set Fractionation
start position to Out 2 in all phases that use fractionation). This requires that you change
the outlet tubing and fraction containers on re-used positions between phases. It may
be advisable include a Miscellaneous phase to set a Pause timer with a message, to
remind you to change the containers.

76

ÄKTA pilot 600 User Manual 29274559 AA

4 Operation

4.2 Fractionation

Содержание AKTA pilot 600

Страница 1: ...KTA pilot 600 User Manual...

Страница 2: ...Column valve 51 3 9 Conductivity monitor 53 3 10 UV monitor 55 3 11 pH monitor optional 57 3 12 Outlet valves 59 4 Operation 60 4 1 Sample application 61 4 1 1 Background considerations 63 4 1 2 Appl...

Страница 3: ...ce tests 120 6 1 When to run performance tests 122 6 2 How to run performance tests 126 6 3 How to evaluate performance tests 128 7 Troubleshooting 129 7 1 System leakage test failure 130 7 2 System b...

Страница 4: ...otices intended use of the KTA pilot 600 system and lists of associated documentation In this chapter This chapter contains the following sections See page Section 5 1 1 About this manual 6 1 2 Import...

Страница 5: ...controlled by a PC running UNICORN system control software version 7 3 or higher For detailed information about the system control software see the UNICORN user documentation For information about ch...

Страница 6: ...tography columns of varying sizes detection and monitoring of UV absorbance conductivity and pH fractionation of column eluate The system is intended for process development scale up and scale down of...

Страница 7: ...nd Technical Manual Safety notices This user documentation contains safety notices WARNING CAUTION and NOTICE concerning the safe use of the product See definitions below WARNING WARNING indicates a h...

Страница 8: ...mation that is important for trouble free and optimal use of the product Note A tip contains useful information that can improve or optimize your procedures Tip 8 KTA pilot 600 User Manual 29274559 AA...

Страница 9: ...A pilot 600 Operating Instructions System overview site requrements and instructions for moving the system within the same building Instructions for basic maintenance and troubleshooting Additional in...

Страница 10: ...enta tion Diagram Technical data for the system GS General Specification Physical layout Provides dimensional data AD Assembly Drawing Description of process related compo nents including wetted mater...

Страница 11: ...ons on how to perform a run UNICORN System Control Manual1 Instructions for setting up and using the UNICORN OPC server UNICORN OPC User Manual 1 Current UNICORN version is added to the title of the m...

Страница 12: ...important and supplementary information about settings is presented in tooltips marked by the information symbol Hover over the symbol to show the tooltip 12 KTA pilot 600 User Manual 29274559 AA 1 In...

Страница 13: ...displayed in this way User documentation and other literature on the web User documentation and other literature related to KTA pilot 600 system may be downloaded from the web Follow the steps below t...

Страница 14: ...Scan the code using your mobile phone or tablet computer to access the product page for KTA pilot 600 Select documents to download under RELATED DOCUMENTS 14 KTA pilot 600 User Manual 29274559 AA 1 In...

Страница 15: ...ssories and the Instrument Configuration software In this chapter This chapter contains the following sections See page Section 16 2 1 Description of the KTA pilot 600 instrument 19 2 2 Flow path 21 2...

Страница 16: ...ctory KTA pilot 600R is delivered with the additional instrument specific detailed docu mentation Installing removing or moving modules will make the documentation invalid The instrument version is in...

Страница 17: ...00S red or 600R blue 1 pH pH monitor 2 Outlet Valve W 1 2 3 Outlet valve 1 3 port 1 can be used for waste 3 Outlet Valve 4 5 6 Outlet valve 4 6 4 Cond Conductivity monitor 5 Outlet Valve 7 8 9 Outlet...

Страница 18: ...B5 B6 Inlet valve B4 B6 13 Fixed plastic feet 14 Adjustable feet 15 Pump A System pump A 16 Pump B System pump B 17 Flow restrictor including system pressure sensor 18 Mixer Mixer module 19 Mixer Val...

Страница 19: ...wing illustration shows a schematic diagram of the flow path Optional compo nents are marked with dashed outlines B1 B2 B3 A1 A2 A3 A5 A4 A6 A8 A7 A9 B5 B4 B6 1 W 2 3 P Cond Cond UV pH P A P R B A 1 I...

Страница 20: ...sor optional 10 Column valve including pre column and post column pressure sensors 11 Conductivity monitor with temperature sensor 12 UV monitor 13 pH module optional 14 Outlet valve with 3 ports Can...

Страница 21: ...instrument may be obtained from the KTA pilot 600 product pages on www gelifesciences com akta The table below lists some examples Illustration Accessory Socket screw wrench 7 mm Tubing cutter SNAP co...

Страница 22: ...ained Use the wider groove for i d 4 8 mm tubing and the narrower for i d 3 2 mm tubing 2 Optional accessories The following optional accessories are available Extension box for optional modules Exten...

Страница 23: ...he external I O box can be used to connect other equipment in order to measure pa rameters such as refractive index light scattering and fluorescence The I O box can communicate with external equipmen...

Страница 24: ...e used to mount columns the Extension box and the I O box The stand includes holders to organize the tubing The following illustration shows the Extension stand with a HiScale column attached 24 KTA p...

Страница 25: ...inge Follow the instructions below to construct a secure connection from a Luer syringe to a module inlet It is important that the connection is secure to avoid leakage of potentially hazardous liquid...

Страница 26: ...5 16 Male SNAP 3 2 mm union part no 29274572 included with accessories 2 Connect the 5 16 Male union to a Luer 5 16 Female union part no 11002707 ordered separately 3 26 KTA pilot 600 User Manual 292...

Страница 27: ...he instrument Strategy The graphic elements which are used to build the Process Pictures in the Method Editor and System Control modules Process Picture Predefined phases and methods adapted to the av...

Страница 28: ...ument Configuration for the system is downloaded and installed The system will be unusable if an incorrect Instrument Configuration is installed 28 KTA pilot 600 User Manual 29274559 AA 2 System descr...

Страница 29: ...nstallation instructions can be found in respective module installation instruction In this chapter This chapter contains the following sections See page Section 30 3 1 External air sensors optional 3...

Страница 30: ...the alarm of an external air sensor is enabled in UNICORN and air is detected the pumps pause and the text AIR is shown in red color in the Process Picture When the alarm of the external air sensor i...

Страница 31: ...page60 Air sensor sensitivity The air sensor has two sensitivity levels High that detects down to approximately 30 L bubbles and Normal that detects down to approximately 1 mL bubbles The level Normal...

Страница 32: ...ctions in a method run for example in the method settings overrides the selec tions made in system settings 32 KTA pilot 600 User Manual 29274559 AA 3 Description of modules 3 1 External air sensors o...

Страница 33: ...g the chromatography run Illustration The illustration below shows the front of the inlet valve module for pump A 5 1 4 2 3 Description Part A1 inlet 1 A2 inlet 2 A3 inlet 3 Outlet to the right pump h...

Страница 34: ...nning isocratic dual pump flow make sure that the same buffer solu tion is pumped in pump A and pump B For example place the A and B inlets in the same buffer container See Section 4 4 Dual pump flow...

Страница 35: ...shows the front of pump A 5 6 7 8 2 3 4 1 Description Part Rinsing solution inlet from the rinsing solution bottle 1 Inlet from Inlet Valve module 2 Rinsing solution inlet from left pump head 3 Inlet...

Страница 36: ...and pump heads work alternately to give a continuous low pulsation liquid delivery The two system pumps can be used individually or in combination to generate isocratic or gradient elution in purific...

Страница 37: ...system pressure Illustration The illustration below shows the front of the flow restrictor module 1 2 3 4 5 6 Description Part Inlet from system pump A left pump head 1 Inlet from system pump A right...

Страница 38: ...from pump A for sample application The system pressure sensor is located inside the flow restrictor module This sensor measures the pressure near the outlet and UNICORN compares the value with the hi...

Страница 39: ...tration below shows the front of the mixer valve module left and the front of the mixer module right 4 7 6 3 2 1 5 Description Part Inlet from the previous module 1 Outlet to the mixer 2 Mixer inlet 3...

Страница 40: ...ly at start and end of a method If it is by passed it does not start When to use Some examples with recommendations to use the mixer or not are shown in the table below Comment Recommended Example Str...

Страница 41: ...duced into the flow path The purpose of the air sensor in the air trap valve is to detect and report all air in the flow path Air reaching the column could decrease the column performance Illustration...

Страница 42: ...of the air trap valve It detects air even when the air trap is by passed If air is detected in the flow path its presence is indicated in the Process Picture If air is detected in the flow path and th...

Страница 43: ...m select Alarm Air Tap valve air sensor in the System Settings dialog box select Enabled and click OK see illustration below Any selections in a method run for example in the method settings overrides...

Страница 44: ...nsor If the air sensor alarm is enabled the run is paused Note Check if the air trap has run out of liquid Make sure that the the air trap is filled before setting it in line The recommen dation is to...

Страница 45: ...filter see the KTA pilot 600 Operating Instructions Illustration The illustrations below show the two in line filter options a stainless steel housing and an ULTA Capsule CG filter from GE 1 2 3 Descr...

Страница 46: ...lter mounting kit available from GE This mounting kit is designed for ULTA Capsule CG filters The pressure limit for these filters is 0 5 MPa see the KTA pilot 600 Operating Instructions for how to se...

Страница 47: ...Intelligent packing to automatically pack AxiChrom columns Illustration The illustration below shows the front of the column valve module 1 10 3 4 5 6 7 8 9 2 Description Part Inlet from the previous...

Страница 48: ...of membrane valves to control the flow path and two integrated pressure sensors to monitor the pressure over the columns The illustrations below show schematic flow paths inside the column valve modu...

Страница 49: ...first four column run options make it possible to run a column separately with up or down flow The Column 1 to column 2 downflow and Column 2 to column 1 downflow options make it possible to run two...

Страница 50: ...sated This affects pressures at higher flow rates 600 mL min There are four pressure signals see the image and table below A B C D Purpose Raw compensated Signal Part Used to protect the column hardwa...

Страница 51: ...of the conductivity monitor modules The two modules are physically identical Pre column 1 2 3 4 Description Part Inlet outlet 1 to 4 Function The conductivity monitors can be used together with watch...

Страница 52: ...r the chromatography run to monitor that column equilibration is completed by comparing conductivity levels before and after the column to monitor that all sample has been wash out from the air trap b...

Страница 53: ...below shows the front of the UV monitor module 2 1 3 4 Description Part Inlet port 1 Outlet port 2 Detector unit 3 Mounting pins for the in line filter 4 Function The UV monitor is used to measure the...

Страница 54: ...mL Turning off the lamp The UV lamp can be turned off manually if not needed during a run for example during column or system conditioningor equilibrationover long time cleaning column or system or s...

Страница 55: ...the pH of solutions used in the run Illustration The illustration below shows the front of the pH monitor module 5 1 2 3 4 Description Part Dummy electrode 1 pH electrode in storage position 2 Inlet...

Страница 56: ...er the column To protect sensitive monitors after the column the post column pressure has an alarm level of 0 8 MPa This alarm is independent of other settings Cleaning storing andcalibration For inst...

Страница 57: ...llustration The illustration below shows the front of two outlet valve modules the mandatory module above and the first optional module below 1 2 3 4 5 6 7 8 9 Description Part Inlet 1 Outlet leading...

Страница 58: ...e connected to the inlet of next module inlet Keep the tubing between the outlet valve modules as short as possible For more information see separate installation instructions for the outlet valve mod...

Страница 59: ...ders additional topics related to operation of the instrument In this chapter This chapter contains the following sections See page Section 60 4 1 Sample application 71 4 2 Fractionation 77 4 3 Intell...

Страница 60: ...nstructions This section gives more in depth infor mation on sample application techniques In this section This section contains the following subsections See page Section 61 4 1 1 Background consider...

Страница 61: ...ample is concentrated by adsorption on to the chro matography resin during sample application The volume and concentration of sample is not critical provided that the capacity of the column is not exc...

Страница 62: ...op 150 mL Sample application phase A Sample Application phase is included in all predefined chromatography methods The sample will be automatically applied to the column when the method is run Selecti...

Страница 63: ...roperties on page 63 or manually see Priming the inlet tubing manually on page 66 Apply the sample according to the phase properties in the Sample Applica tion phase 2 Finalize the sample application...

Страница 64: ...repro ducible sample application For adsorptionchromatography select Finalizesample injection to make sure that all sample is applied to the column before the next phase starts For size exclusion chro...

Страница 65: ...the inlet tubing with sample by pumping the specified volume of sample Enter a volume larger than the volume of the inlet tubing Prime sample inlet with When this option is selected select Pump wash a...

Страница 66: ...ring sample application is generally rele vant only for adsorption chromatography Fractionation settings Priming the inlet tubing manually The sample inlet tubing can be primed manually if desired usi...

Страница 67: ...sample front may be difficult to follow if the flow rate is too high 3 When the sample front has passed through the inlet valve click Pause 4 Switch the inlet valve to buffer inlet 5 Click Continue c...

Страница 68: ...with downflow through the column Note Mounting the Superloop The Superloop should be mounted close to the column valve to keep connecting tubing short Mounting in an KTA accessory holder on a module...

Страница 69: ...Description Part Eluent chamber 1 Sample chamber 2 Sample Application phase properties To apply sample from a Superloop select Inject sample from Superloop manual load in the Phase Properties for the...

Страница 70: ...pumped into the Superloop eluent chamber during sample application Use the same inlets as Method Settings Select this option to fill the system flow path with the buffer before applying the sample to...

Страница 71: ...ype of fractionation Whether and how fractionation will be performed is determined by the three options to the left of the panel Description Setting Continues fractionation using the settings from the...

Страница 72: ...for this parameter to avoid splitting peaks into several fractions Peakfractionation volume Fractionation will stop at the end of the phase even if fraction ation in the next phase is set to Continue...

Страница 73: ...Advanced settings determines the maximum number of fractions that will be collected Any outflow after the maximum number of fractions has been collected will be directed to Outlet 1 Waste Check Use a...

Страница 74: ...hold value for detection of peak start The peak start is detected when the signal exceeds the specified value Start level Start slope Set the threshold values for detection of peak end The peak end is...

Страница 75: ...uired from the phase library Note Conditional Fractionation can be used to set conditions for fractionation start and stop based on one signal e g conductivity while performing peak fractionation base...

Страница 76: ...r of fractions Use Conditional Fractionation and Peak fractionation to optimize the number of fractions If the number of fractions exceeds the number of available outlets it is possible to re use outl...

Страница 77: ...this section you will get general information for how to handle the packing procedures For more information read about the Intelligent Packing phase in the UNICORN help and documentation Illustration...

Страница 78: ...thod settings Make sure that Column position and Default curve to show are correct 3 Tip For information about the settings click on the Phase Properties tab and then press F1 Note Text editing in Met...

Страница 79: ...tings Tip When GE verified packing settings is selected you can enter the slurry concentration and click Slurry Recipe to get a slurry recipe calculated to give your target bed height 6 Verify the inl...

Страница 80: ...umn Tip In the Intelligent Packing phase of your packing method click on Show Column Connection to find an illustration of the connections Run your packing method in the System Control module follow t...

Страница 81: ...r Note Gradient applications It is possible to use Dual pump flow to make gradients above 600 mL min The illustration below shows the gradient range for KTA pilot 600 Above 600 mL min yellow part of t...

Страница 82: ...box enter System flow value and click Set 2 Using dual pump flow manually Follow the steps below to start dual pump flow using manual instructions Action Step Select System Control Manual Execute man...

Страница 83: ...ystem flow instruction 2 In the Instruction Box pane of the Text Instructions tab select Pumps Dual pump flow 3 Enter your parameters Verify in the graph shown in Gradient applications on page 81 that...

Страница 84: ...ns When to adjust pressure control Pressure control requirements in the KTA pilot 600 system depend on whether the Air trap is bypassed or in line as well as on flow rate Pressure control is most impo...

Страница 85: ...section of Manual instructions 3 Run Pumps System flow at a suitable flow rate to test the effect of your adjustments 4 When you are satisfied update the pressure control parameters in System Settings...

Страница 86: ...online help for the pressure control parameters 3 Click OK 4 Activate pressure control in a method To activate pressure control in a method select Control the flow to avoid overpressure in the Phase...

Страница 87: ...to avoid over heating Place the computer in room temperature If the KTA pilot 600 instrument is placed in a cold room use a cold room compat ible computer or place the computer outside the cold room a...

Страница 88: ...ffers for pH calibration Temperature differences might for example increase outgassing Higher viscosity Generally cold solutions have higher viscosity than warm solutions The flow rate might need to b...

Страница 89: ...3 5 2 Maintenance schedule 95 5 3 Cleaning procedures 100 5 4 Calibration procedures 109 5 5 Replacement procedures Maintenance tasks Refer to the KTA pilot 600 Operating Instructions for instructions...

Страница 90: ...potentially explosive atmosphere the user must test the piping system for leakage at maximum operating pressure Always perform a leakage test after assembly or maintenance Always perform a leakage tes...

Страница 91: ...rom the instrument before performing main tenance NOTICE Do not allow solutions which contain dissolved salts proteins or other solid solutes to dry out in the UV flow cell KTA pilot 600 User Manual 2...

Страница 92: ...ion of automatednotificationsformaintenanceactions basedon calendartimeandoperational statistics A set of predefined notifications is provided and custom notifications may be created Using the Mainten...

Страница 93: ...lu tion daily if buffer is used or weekly if 20 ethanol is used Daily or weekly Section 5 4 1 Reset the pressure sensors on page 101 Reset the pressure sensors Section 5 3 2 Clean the UV flow cell on...

Страница 94: ...ductivity monitor on page 104 Calibrate the conductivity monitor Section 5 4 1 Reset the pressure sensors on page 101 Reset pressure sensors See the KTA pilot 600 Operating Instructions Replace tubin...

Страница 95: ...ions For column cleaning and storage procedures refer to the column instructions Note Refer to the computer documentation for recommendations for cleaning the computer equipment In this section This s...

Страница 96: ...to clean the conductivity flow cell The same procedure applies to both pre and post column conductivity monitors WARNING Corrosive substance NaOH is corrosive and therefore dangerous to health When us...

Страница 97: ...in the flow cell for about 15 minutes 5 Flush the flow cell again with 25 to 30 mL distilled water 6 Disconnect the Luer to SNAP connector and waste tubing from the conduc tivity monitor and reconnec...

Страница 98: ...rial The following materials are required Luer to SNAP connector see Luer SNAP 3 2 connectors on page 25 Waste container Syringe 25 to 30 mL Detergent solution e g Decon 90 Deconex 11 or RBS 25 dilute...

Страница 99: ...ut five times with 10 mL detergent solution Tip Warm the detergent solution to 40 C for enhanced cleaning effect 4 Allow the detergent solution to remain in the flow cell for about 15 minutes 5 Flush...

Страница 100: ...an be performed using the System Control module in UNICORN software In this section This section contains the following subsections See page Section 101 5 4 1 Reset the pressure sensors 104 5 4 2 Cali...

Страница 101: ...val The pressure sensors should be tested every week or when the the ambient temperature has changed by more than 5 C Reset the pressure sensors if the atmospheric pressure reading is outside the rang...

Страница 102: ...ow the steps below to reset the pressure sensors Reset the pressure sensors one at a time Action Step Make sure that the appropriate tubing see Tubing connections on page101 is disconnected so that th...

Страница 103: ...3 If the Current value is outside the range 0 02 MPa 0 2 bar 2 9 psi click Reset pressure 4 Result The pressure reading is reset to zero Reconnect the tubing to the pressure sensor 5 KTA pilot 600 Use...

Страница 104: ...is as follows When to perform Calibration type When the signal is unstable or when you suspect that the value is incorrect User calibration When user calibration fails Factory calibration Required ma...

Страница 105: ...the top of the conductivity monitor and replace it with a piece of tubing leading to the waste container 3 W Select System Settings in UNICORN System Control module 4 In the Instructions list select C...

Страница 106: ...11 If a certified conductivity standard solution is used use the supplied theoretical conductivity value If a manually prepared 1 00 M NaCl calibration solution is used see the graph for conductivity...

Страница 107: ...onductivity cell constant is restored to the factory default value The conductivity cell constant is printed on the packaging of the Conductiv ity monitor Conductivity values of 1 00 M NaCl The graph...

Страница 108: ...7 80 85 90 95 97 Temperature C Conductivity mS cm Conductivity of 1 00 M NaCl at 20 30 C 108 KTA pilot 600 User Manual 29274559 AA 5 Maintenance 5 4 Calibration procedures 5 4 2 Calibrate the conducti...

Страница 109: ...ixer module 114 5 5 3 Replace the UV monitor flow cell 118 5 5 4 Replace valve fronts Precautions WARNING Disconnect power Always disconnect power from the instrument before replacing any component on...

Страница 110: ...service WARNING Decontaminate before maintenance To avoid exposing personnel to potentially hazardous substances clean and sanitize the KTA pilot 600 system before maintenance or service 110 KTA pilot...

Страница 111: ...ss tip may break and cause injury Follow the steps below to replace the pH electrode Action Step Disconnect the pH electrode cable from the connection on the front of the pH monitor 1 Unscrew the lock...

Страница 112: ...r module Action Step Disconnect the tubing from the mixer chamber 1 Pull the mixer chamber away from the module front 2 Remove the retaining screw that holds the module in the instrument chassis see t...

Страница 113: ...e tubing to the mixer chamber 5 Flush the system with water or buffer to remove the air from the new module 6 KTA pilot 600 User Manual 29274559 AA 113 5 Maintenance 5 5 Replacement procedures 5 5 2 R...

Страница 114: ...er make sure that the UV lamp is disconnected or that the power is discon nected Maintenance interval Replace the UV flow cell when a flow cell with a different path length is required or if the cell...

Страница 115: ...disconnected Pull off the detector with the flow cell off the instrument 4 Note Make sure that the flow cell does not come into contact with any liquid and that no liquid enters the UV detector or the...

Страница 116: ...from dust Take care not to damage the flow cell 6 Fit the replacement UV flow cell to the detector 7 Fit the detector with the replacement flow cell to the instrument front panel Pull the latch upwar...

Страница 117: ...ances used Fol low local and or national regulations for safe operation and maintenance of the product Action Step Wipe the optical fiber connectors with a lens paper moistened with iso propanol 1 Wip...

Страница 118: ...lacing if they are damaged Procedure Obtain a new valve front from GE then follow the procedure for replacing valve mem branes described in the KTA pilot 600 Operating Instructions 118 KTA pilot 600 U...

Страница 119: ...n general terms how to access run and assess performance tests Detailed on screen instructions are provided in the UNICORN software for each test In this chapter This chapter contains the following se...

Страница 120: ...If any of the tests fails correct the cause of the failure before continuing Run the test s associatedwith the specificmodule Aftermoduleadditionorreplace ment Run the test for the specific valve Aft...

Страница 121: ...er Test order Condition Run valve module tests in the following order System leakage test fails Inlet valve Outlet valve Column valve Air trap valve Mixer valve Ensure that Air Trap valve and Column v...

Страница 122: ...the system pumps at a low flow rate with the flow path closed at some point Ensure that the system pressure limit is set to the default value 2 0 MPa before running performance tests Procedure Perfor...

Страница 123: ...the KTA pilot 600 system Attempting to run a test for a module that is not installed will generate an error message Click Run Performance Method 4 Read the Method Notes carefully and make sure that al...

Страница 124: ...any instructions that are shown on the screen 8 The progress of the performance test is shown in the chromatogram pane and can be customized in the same way as any chromatogram Required materials Mat...

Страница 125: ...Reference capillaries are provided with the system and may also be obtained separately from GE KTA pilot 600 User Manual 29274559 AA 125 6 Performance tests 6 2 How to run performance tests...

Страница 126: ...run The tests may fail if the system pressure limit is set to a lower value Inspect the system visually for leaks while the test is running If there is significant leakage the test report will indica...

Страница 127: ...tests Note Test results The performance test data are displayed in the System Control module as the test pro gresses and are saved in the same way as results from chromatography runs Examina tion of...

Страница 128: ...leshooting is described in the KTA pilot 600 Operating Instructions If you have problems that cannot be resolved using the information provided here contact your GE service organization In this chapte...

Страница 129: ...g connections and valves in the flow path Correct the problem according to the table below and rerun the System leakage test to make sure the leakage is corrected If the problem persists after correct...

Страница 130: ...s not kinked or obstructed Replace any kinked tubing Locate the source of increased backpressure by moving the outlet tubing to the outlet port of successive modules starting with the outlet valve s a...

Страница 131: ...Note Corrective action Failed test Make sure that tubing to and from Pump A and Pump B is not obstructed Gradient test Restart the instrument to perform automatic wavelength calibration and light int...

Страница 132: ...t zero If the test is performed in a cold room the temperature compensation factor should be 2 1 Curve amplitude stability conductivity The temperature compensation factor is accecssed through System...

Страница 133: ...re Replace the valve membranes see the KTA pilot 600 Operating Instructions Valve tests indicate leakage If the problem persists replace the valve module Where appropriate clean the flow cell in the m...

Страница 134: ...00S Product Documentation for KTA pilot 600S systems Detailed information specific to the individual system is provided separately with KTA pilot 600R systems This chapter gives additional specificati...

Страница 135: ...1 Pump two pump heads 4 9 Pump rinse system 3 9 Flow restrictor 3 9 Air trap valve 73 7 Air trap 3 4 Mixer valve 5 4 Mixer chamber 15 0 Column valve 0 4 Conductivity monitor 0 2 UV monitor 2 0 mm flo...

Страница 136: ...e following topics System recommendations Literature Service Training Accessories Ordering information Spare parts Your local GE representative can suggest recommended spare parts Contact information...

Страница 137: ...bration 104 Conductivity values 1 00 M NaCl 107 Connectors 21 D Delta column pressure 50 Documentation on the web 13 Documentation package regulatory version 10 standard version 9 Dual pump flow 81 fo...

Страница 138: ...ng 111 pH monitor 55 replace the pH elec trode 111 PI regulation 84 Pre column conductivity moni tor 52 Pre column pressure 50 Prerequisites 7 Pressure control 84 parameters 85 Pressure monitors calib...

Страница 139: ...e help 11 User documentation 9 access from mobile units 14 Using intelligent packing 80 UV flow cell cleaning 98 optical fiber connec tions 116 replacing 114 UV monitor 53 clean UV flow cell 98 replac...

Страница 140: ...ral Electric Company All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is availab...

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