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4.9. Basic Control by Keyboard
After unit start (mode START) display shows unit
type.
Then the unit turns to mode IDD, pump motor is in
idle status.
Main screen
–
isocratic
pump mode.
Main screen
–
gradient
pump mode.
Gradient mode is detected automatically by cable
interconnection with a box after unit start.
4.10. Pump Initiation
The unit is filled with isopropanol, it is necessary to
rinse it out
and fill the system with
mobile phase and
degas
the entire system.
Loosen purge/degassing valve by turning it by
90°.
Slip a plastic syringe on purge/degassing valve,
the syringe (50ml) is a part of pump accessories.
In gradient mode is necessary to check, whether appropriate valve (A, B, C or D) with mobile
phase is opened. By pressing key PURGE and then pressing key PUMP is started pump washing.
Suck in the mobile phase with the plastic syringe as long as bubbles come out from the pump. It is
possible to switch the valves during washing, by pressing keys with arrows, this allows the liquid to
penetrate to all necessary channels Press key PUMP again to stop the pump washing. Tighten the
purge/degas valve and take out plastic syringe.
For repeated gas purging/degassing it is possible
to put the waste tubing on purge valve.
If waste tubing is put on purge/degas valve, it is
not possible to close the unit door.
In chapter
it is possible to set parameters
foe washing.
Now the flow can be set by pressing key FLOW.
Use arrows left and right to move with cursor and
arrows up and down to change value. Press
ENTER key to confirm the value, press PUMP to
start the pump. Press PUMP again to stop