ECP2050
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6.7.2.1. Pressure Sensor
In this menu item is calibrated zero of pressure sensor. For user is available only item
OFFSET AUTO
, where is pressure sensor zero set automatically. Calibration is proceeded on
stopped pump
. At first loosen purge/degassing valve and then press ENTER for proceeding
automatical calibration.
Caution:
When correcting offset often, contact service.
6.7.2.2. Flow
In this menu item is calibrated mobile phase flowrate. For user is available only automatical
flowrate calibration
FLOW AUTO
.
Caution:
For correct calibration it is necessary that the pumping system is rinsed
properly. There may not be any bubbles and check valves must work
correctly (if there are used check valves without springs, pressure should
be at least 1 MPa (145 psi).
Caution:
For calibration are set conditions (pressure, flowrate, mobile phase) as
close as possible to the conditions used during common unit use.
In menu item
FLOW AUTO
is proceeded flowrate calibration in six steps. Unit makes
calculations by itself based on measured time, and volume passed through during this time, which
is recorded. To the calculations it is necessary to add value of reference (exact) volume. Based on
calculated deviation unit sets flowrate correction.
Prior to calibration is the unit connected according to flow chart below. Reference volume is
the volume of accurately measured volumetric flask class A (according to ISO 1042), where is
dropping mobile phase flowing through the unit. After filling exact volume of the volumetric flask is
the measuring finished.
F
LOW
C
HART OF
S
YSTEM FOR
F
LOWRATE
C
ALIBRATION
1.Container with liquid; 2. Pump; 3. Column (caution some are not intended for pure water); 4. Pressure
control valve (may be used instead of column); 5. End of capillary, 6. Volumetric flask.
Note:
We recommend to use freshly degassed mobile phase.
Selection of Volumetric Flask Volume
Volume of volumetric flask should be chosen based upon set flowrate, so that the measuring
takes 5 up to 15 min.
