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ECP2050

ECOM spol. s r.o., Trebonicka 239, 252 19 Chrastany u Prahy, Czech Republic

tel: +420 221 511 310, fax: +420 242 498 212, [email protected], http://www.ecomsro.com/

IČO: 411 92 192, DIČ: CZ411 92 192

User´s Guide

ECP2050

REPARATIVE

HPLC

PUMP

Содержание ECP2050

Страница 1: ...ol s r o Trebonicka 239 252 19 Chrastany u Prahy Czech Republic tel 420 221 511 310 fax 420 242 498 212 info ecomsro cz http www ecomsro com I O 411 92 192 DI CZ411 92 192 User s Guide ECP2050 PREPARA...

Страница 2: ...Form nek First issue 00 05 11 2019 Dominika Pavlov Jaroslav Form nek Address change in the footage of the first page the in chapters 13 and 15 01 08 04 2020 Dominika Pavlov Jaroslav Form nek Declarat...

Страница 3: ...Backwash 13 4 5 1 Simple Manual Use 14 4 5 2 Automatic Backwash 14 4 6 Assemblage of Waste System 15 4 7 Connection on Unit Rear Panel 16 4 8 Start of Unit 17 4 9 Basic Control by Keyboard 18 4 10 Pum...

Страница 4: ...net 44 6 5 4 User Text 44 6 5 5 Pump 44 6 5 6 Parts 44 6 6 Diagnostics 44 6 6 1 Errors 44 6 6 2 Warnings 45 6 6 3 Power 45 6 6 4 Cooling 45 6 6 5 Interface 45 6 6 6 Leakage State 45 6 6 7 Pump 46 6 6...

Страница 5: ...CP2050 5 64 11 3 Cleaning Check Valves 60 11 4 Storage and Transport 60 12 SPARE PARTS 61 13 WARRANTY AND POST WARRANTY REPAIRS 62 14 TECHNICAL PARAMETERS 63 15 APPENDIX 1 DECLARATION OF COMFORMITY 64...

Страница 6: ...nnection When entering menu items designated for gradient mode e g Menu Parameters Concentration and pump is isocratic mode unit informs too 1 2 Product Version ECP2050 Preparative HPLC pump Cat No AC...

Страница 7: ...1 8 1000 mm with filter 20um with lid FEP tubing ID 1 16 volume 1986 L only tubing PEEK screw 1 8 KEL F ferrule 1 8 thread 28 UNF PP lid on tray GL45 with PA plug with tubing holes PE filter PEEK scr...

Страница 8: ...There is a danger of its damage Caution Never dismount unit cover There is nothing needing customer service or maintenance Caution The pump is filled with isopropanol after production or service Caut...

Страница 9: ...washing head 5 Leakage sensor 6 Drain channel with cone for connecting waste system 7 Door hinges 8 Slots for tubing and capillaries 9 Display 10 Status LED PUMP green for indication of pump motor st...

Страница 10: ...rameter value evokes QUICK DIAGNOSTICS screen ESC Returns one step back without saving changes B D A C Direction keys Using arrows up down increases decreases value of set parameter A B C D direct cho...

Страница 11: ...tion or service Isocratic connection of pump inlet Connect inlet tubing with filter Snap the tubing to the socket for tubing and capillaries Set of inlet tubing with a lid and filter picture on the ri...

Страница 12: ...stem accessories and shall be ordered separately The injection valve holder is constructed for mounting two different types of valves and with hole for outlet capillary from pump to injection valve Rh...

Страница 13: ...the cable through the window and connect to connector START or IO Interface connector on unit rear panel Make configuration see 6 4 3 4 5 Piston Backwash Caution If you are using puffers or other crys...

Страница 14: ...side Now once in a while during work with the pump push through the pump some liquid to the washing system approx 1x per hour 5 ml 4 5 2 Automatic Backwash Connect both Washing heads in series using t...

Страница 15: ...chapter 6 6 6 is shown the leakage sensor status WASTE SET ECP2050 1 Silicone tubing 2 m with connector Y and I 2 Connector Y with two pieces of silicone tubing 3 Connectors L into the drain channel...

Страница 16: ...icon tubing part 1 according to your needs Second connector L is pushed into the drain channel of pump horizontally and is connected with second tubing of connector Y of part 1 Third tubing of part 1...

Страница 17: ...plugged into PC or computer network element Connector USB serves for plugging USB cable for interconnection with PC Cable is not among the unit accessories Plug serial line cable into connector RS232...

Страница 18: ...mobile phase is opened By pressing key PURGE and then pressing key PUMP is started pump washing Suck in the mobile phase with the plastic syringe as long as bubbles come out from the pump It is possib...

Страница 19: ...set pressure units 1 00ml min Actual flowrate value 13 0MPa Actual pressure value IDD Abbreviation of actual pump mode Actual piston position is displayed after first finished pump rotation During fl...

Страница 20: ...door hinges the door is supplied apart The door should be slipped on the hinges in opened position see pictures below Door mounting must be easy no strength needed otherwise the hinges may get damage...

Страница 21: ...rientation in menu is in next chapter shown menu flow diagram and description of menu parts MENU FLOW DIAGRAM 6 1 Parameters For setting basic parameters of the pump such as flowrate pressure limits c...

Страница 22: ...ed for unattended pump run to avoid uncontrolled outflow of mobile phase in case of capillaries disconnection 6 1 3 Concentration Concentration of mobile phase can be set for each valve in volume perc...

Страница 23: ...ng operations may be this beep generated at the beginning and at the end of an operation In default setting are all sounds set on 6 2 2 Purge In each sub menu can be set possibilities of pump purge 6...

Страница 24: ...nd optimal curve of pump rotation speed so that pulsation meets the condition set in Menu Preferences Compensation Pressure pulsation limit Pump learning must be started in Menu Control Start compens...

Страница 25: ...contains reference and measuring sensor If measuring sensor is immersed in liquid sensor generates a signal Processing of this signal depends on actual setting The pump also processes the leakage sen...

Страница 26: ...in In menu item Menu Diagnostics Puls is displayed pulsation status and information about values of parameters under which compensation learning took place see 6 6 9 After setting different flowrate t...

Страница 27: ...arting from firmware FW verzion 4 x Pump enable setting of 12 gradient programs directly in the unit Programming offers a tool for gradient concentration course control and controlling mobile phase fl...

Страница 28: ...pump runs program reinitiates for restart or during running probram by evoking menu item Menu Control Reinit program and subsequently Menu Control Stop program Programming mode remains saved after tu...

Страница 29: ...nated Displayed below x y A B C D Concentration is changed with visible countdown and count up of in time for each valve or step changes or is constant CHANGES ON MAIN SCREEN FROM INITIALIZATION TO PR...

Страница 30: ...s shown change from to I total time stops and flashes step time stops pump is running Mobile phase concentration change stops By pressing PUMP status changes to held I changes to II and follows manipu...

Страница 31: ...accelerated option of program initialization by pressing key PUMP for 3 s For RUN at first press PUMP shortly in order to stop pumping change to and then after pressing key PUMP for 3 s program initi...

Страница 32: ...d program mode is abandoned System enable to create and save 12 programs Default status is EMPTY 6 3 4 1 Load Program By uploading a program into unit memory is the unit prepared for initialization by...

Страница 33: ...Program Creating Choose desired sequence number of program X and press ENTER Note We recommend to set at first menu item VALVE USED and other functions before setting TIME TABLE Note We recommend to s...

Страница 34: ...rate remains set from program and END STEP REPEAT sets initialization phase flowrate DISABLED program does NOT change flowrate In column FLOW in TIME TABLE is displayed Running program works with actu...

Страница 35: ...start time of step flowrate concentration in see table above 2 Exit TIME TABLE ESC 3 Controlling alterations made enter TIME TABLE New step is enlisted on its position in time axis of step beginnings...

Страница 36: ...Programs Choose number of modified program X S xx press ENTER to enter and work as during first setting When leaving modified program X S xx you will always be asked whether to save changes or not No...

Страница 37: ...nges are not applied to running program Program concludes according to original setting By reloading the program in menu item LOAD PROGRAM the changes are applied Note We do not recommend to combine s...

Страница 38: ...t modification during step 0 is the course represented by curve ES0 After second modification during step 2 is the course represented by curve ES2 Flowrate is displayed by curve F Start times of steps...

Страница 39: ...ally by pressing ENTER in menu item OPEN GRADIENT VALVES Selected valve opens and changes from OPEN GRADIENT VALVES to CLOSE GRADIENT VALVES After start and stop the valves close again 6 4 IO Config 6...

Страница 40: ...s necessary to set on the unit and also in PC suitable connecting parameters e g ECP2000 IP 192 168 91 30 MASK 255 255 255 0 and PC IP 192 168 91 1 MASK 255 255 255 0 Caution When using static address...

Страница 41: ...ith TTL HC HCT Overvoltage protection up to 12 V In opened status it is on level H 6 DIN1 DIGITAL INPUT 1 Digital input 1 Compatible with TTL HC HCT Overvoltage protection up to 12 V In opened status...

Страница 42: ...through remote control RS232 ETHERNET or SAFETY STOP can control pumping 6 4 3 2 Digital Input 1 DIGITAL INPUT can be used as follows SETTING ACTIONS ON DIGITAL INPUTS Setting Description OFF Digital...

Страница 43: ...6 5 Info In each submenu is displayed information about the unit and its parts The unit contains information about internal components their serial numbers versions etc All information can be read fr...

Страница 44: ...5 6 2 Check Valves L INLET left inlet check valve R INLET right inlet check valve L OUTLET left outlet check valve R OUTLET right outlet check valve 6 5 6 3 Pistons LEFT left piston RIGHT right piston...

Страница 45: ...ons and other behavior is the same as for errors see chapter above 6 6 3 Power In this menu item is displayed value of main supply voltage nominal value is 32 0 V and it should be within the range of...

Страница 46: ...n bandwidth PMAX PMIN per one cycle DEAD Status of optical switch detecting position of top dead center of main left piston CLOSE OPEN MAGV Motor load mean value of maximum allowed load MMAX Motor loa...

Страница 47: ...menu item is displayed status of values for controlling gradient valve GRADIENT VALVE STATUS Item Description FUNCT Box connection ON connected OFF disconnected VALVE Valve used IDRES Measured value...

Страница 48: ...s Terms for exchange and unit maintenance is stated in chapter 11 1 Other replaceable parts are needle and O ring in degassing purging valve This exchange is not recorded by the device Caution Before...

Страница 49: ...back on the piston must be straight Leaning the head to sides may cause seal damage Fasten the screws and mount tubing and capillary back on their place After seal replacement are the steps for mounti...

Страница 50: ...ion Watch washing heads and all connections during run in for any leakage Correct every uptightness In menu item Menu Service Parts replacement Seals tick the seal that has been replaced L PUMPING lef...

Страница 51: ...d one four holes must head up Continue the same way as during dismounting but in reverse order Tight all screws strongly and after starting the pump check all connections for any leakage In menu item...

Страница 52: ...eedle take out metal ring and O ring Take in new O ring After replacement of O ring steps for mounting are the same as for dismounting only in reverse order Caution Needle is screwed into head of dega...

Страница 53: ...during common unit use In menu item FLOW AUTO is proceeded flowrate calibration in six steps Unit makes calculations by itself based on measured time and volume passed through during this time which i...

Страница 54: ...the unit to stabilize for approximately 1 minute Press ENTER to continue TIME ml PRES xx yy MPa xx actual pressure yy pulsation 3 6 Unit is prepared for start by pressing ENTER At the same time start...

Страница 55: ...unit when measuring time of filling volumetric flask t V F F ml min pump flowrate V ml volumetric flask volume t min time for collecting the volume 6 7 2 3 Gradient Gradient calibration is accessible...

Страница 56: ...on Initialization INITIALIZATION INI Temporary mode after unit start Initial screen of unit type displays Basic unit initialization is executing Starting START STA Transitive status after pumping star...

Страница 57: ...Push the fuse holder all the way inside 10 TROUBLESHOOTING Problem Caused by Conditions Solution Leaking liquid from washing heads Damaged seal in pumping head Replace the seal immediately The pump m...

Страница 58: ...lectronic failure Contact service center Flowrate is lower then set value Pressure stability Problems with lower flow rate occur and usually relate to problems with pressure stability see solution abo...

Страница 59: ...dry up surroundings of sensor see 6 2 7 W 17 18 19 20 E 37 38 39 40 SOLVENT A B C D UNDER LIMIT In gradient mode Reported according to setting in 6 2 5 2 Operating error Add mobile phase into reservoi...

Страница 60: ...year if the working conditions are optimal max once per 2 years During unit validation a complete unit control is proceeded and according to observed unit condition can be replaced damaged parts Perio...

Страница 61: ...C 204 UHMWPE40 HST head seal polyethylene with high molecular weight lower chemical resistance high wear resistance 53381000 Check valve inlet and outlet check valve 53380012 Holder of outlet check va...

Страница 62: ...m 13 WARRANTY AND POST WARRANTY REPAIRS Warranty and post warranty repairs are provided by the manufacturer or dealer organization authorized by company ECOM to do this Repair of products in the warr...

Страница 63: ...EK KEL F PE seals Communication RS232 Ethernet LAN USB Display keyboard VFD 140x32 pixels 10 pushbuttons Power supply 100 240 V 50 60 Hz 100 VA Dimensions w x h x d 280 x 135 x 498 mm 11 02 x 5 32 x 1...

Страница 64: ...s were applied when evaluating conformity EN 61010 1 ed 2 2011 A1 2019 idt IEC 61010 1 2010 A1 2016 COR1 2019 EN 61000 6 3 ed 2 2007 A1 2011 Cor 1 2013 idt EN 61000 6 3 2007 A1 2011 AC 2012 EN 61000 3...

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