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12. Technical Specifications

Dimensions

Without consumable: 21.5 x 35 x 11 cm (8.5 x 13.8 x 4.4 in.)

With consumable installed: 22 x 35 x 15 cm (8.7 x 13.8 x 5.9 in.)

Weight

Without consumable: 5 lbs. (2.3 kg) 

With consumable installed: 8 lbs. (3.6 kg) 

Pumping Rate

There are two peristaltic pumps each capable of pumping between 8 µL/min. 
and 15,000 µL/min. utilizing the Corning® MicroDEN® single-use consumable.

Environmental Limits

The Corning MicroDEN unit is designed for use in:

Indoor environments

Ambient temperature between 2°C and 50°C

Humidity between 50% to 100% R.H., non-condensing

Air containing up to 5% CO

2

Altitude up to 2,000 m

Not for use in potentially explosive environments

IP Rating

MicroDEN, IP22

External power supply, IP41

Equipment Ratings

External power supply: 100-240 VAC, 50-60 Hz, 1.5 A

Corning MicroDEN power requirements: 24 VDC ± 10%, 0.9 A (supplied by the 
external power supply)

MicroDEN input connection: 5.5 mm barrel jack, 2.1 x 11 mm center positive

Fuse: 4A, 250V 5 x 20 mm time delay (slow blow)

Overvoltage Category II

Pollution Degree 2

10. Cleaning and Care

The Corning MicroDEN

 

unit is compatible with 70% ethanol and 10% bleach 

solution. Lightly spray the unit with 70% ethanol to disinfect before placing 
in the biosafety cabinet. After use, the unit can be cleaned with 70% ethanol 
or 10% bleach solution. The unit should be stored upright in a cool, dry 
environment when not in use.

11. Troubleshooting

Symptom Solution

The unit does not turn on. 

 Ensure the power cord is fully inserted into the 
unit and wall outlet. A green light will illuminate 
on the AC converter of the power cord when it is 
plugged in properly.

Liquid does not properly 

Inspect tubing and connections to ensure 

perfuse. 

everything is connected.

 

 Check the filters. If the filters become wetted 
during use, they may not allow gas exchange, 
which would cause the bottles to pressurize and 
liquid to not perfuse properly.

 

 Filters supplied with the consumables can be 
replaced with a new 0.2 µm sterile filter with a 
male Luer Lock.

 

 Ensure the tubing is properly placed in the pump. 
The pump has guides that keep the tubing in 
place. Tubing should be placed in the center of 
these guides when the pump is clamped.

Liquid perfuses backwards.   Switch the tubing orientation so the tubing 

coming from the left side inlet bottle enters the 
left side of the pump. The colored marker on the 
tubing should be to the left of each pump and 
match the colored flag next to each pump head 
(Figure 2). 

 

 NOTE:

 Placing the tubing so the colored marker is 

on the right side of the pump will cause liquid to 
perfuse backwards.

Содержание MicroDEN

Страница 1: ...hnical information visit www corning com lifesciences or call 800 492 1110 Outside the United States call 1 978 442 2200 or contact your local Corning sales office Corning Incorporated Life Sciences 8...

Страница 2: ...autoclave this unit Do not perform the sterilization cycle of an incubator with the MicroDEN unit inside Do not use acetone to clean the unit Always use the provided external power supply to power the...

Страница 3: ...nts 1 Corning MicroDEN unit Cat No 431276 requires standard electrical outlet 2 Corning consumable set Cat No 431277 3 Corning storage bottle Cat No 431278 4 Enriched monocytes MOs 5 Differentiation m...

Страница 4: ...t a wall to prevent disturbing the cultured cells The cell culture flasks may be seeded with either enriched MOs or with peripheral blood mononuclear cells PBMCs where the MOs are enriched through pla...

Страница 5: ...ow through the unclamped tube Method 2 Pump Perfusion 1 Unclamp the pumps by pressing upwards at the dotted triangle on the pump head 2 Place the tubing onto the pump rollers The colored tubing marker...

Страница 6: ...emptied on Day 3 1 Turn the Corning MicroDEN unit OFF disconnect the power cord and remove from the incubator 2 Lightly spray the MicroDEN system with 70 ethanol and place inside the biosafety cabine...

Страница 7: ...e new storage bottle containing differentiation medium Disconnect the empty bottle MPCs and connect the new storage bottle MPCs to the inlet side tubing 8 Transfer the Corning MicroDEN system from the...

Страница 8: ...he flasks back onto the thermoform tray with the corner notch of each flask in the upper right position 10 Repeat Steps 1 9 for a second cold buffer wash NOTE Flasks can be harvested individually by u...

Страница 9: ...anol to disinfect before placing in the biosafety cabinet After use the unit can be cleaned with 70 ethanol or 10 bleach solution The unit should be stored upright in a cool dry environment when not i...

Страница 10: ...riginal material or workmanship This warranty does not cover motor brushes fuses light bulbs batteries or damage to paint or finish Claims for transit damage should be filed with the transportation ca...

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