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Fully assembled Corning MicroDEN platform

Corning

®

 MicroDEN

®

 Platform

Instruction Manual

© 

2019 

Corning Incorporated. All 

righ

ts 

reser

ved. 

  9/19 

  CLS-AN-541/11319-30574 

RE

V1

Register your product warranty online at 
www.corning.com/lifesciences/warranty

. 

For more specific information on claims, visit the Certificates page at www.corning.com/lifesciences
Warranty/Disclaimer:

 Unless otherwise specified, all products are for research use only. Not intended 

for use in diagnostic or therapeutic procedures. Corning Life Sciences makes no claims regarding the 
performance of these products for clinical or diagnostic applications.

For a listing of trademarks, visit www.corning.com/clstrademarks. All other trademarks are the property of their respective owners.

For additional product or technical information, visit www.corning.com/lifesciences or 
call 800.492.1110. Outside the United States, call +1.978.442.2200 or contact your local 
Corning sales office.

Corning Incorporated

Life Sciences

836 North St. 
Building 300, Suite 3401 
Tewksbury, MA 01876
t 800.492.1110 
t 978.442.2200 
f 978.442.2476
www.corning.com/lifesciences

A S I A / P A C I F I C
Australia/New Zealand
t 61 427286832
China
t 86 21 3338 4338
f 86 21 3338 4300
India

 

t 91 124 4604000
f 91 124 4604099 
Japan
t 81 3-3586 1996 
f 81 3-3586 1291
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f 82 2-796-9300
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E U R O P E
[email protected]
France
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f 0800 918 636
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f 0800 101 2427
The Netherlands 
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f 020 659 76 73
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t 0800 376 8660
f 0800 279 1117
All Other European  

Countries
t +31 (0) 206 59 60 51 
f +31 (0) 206 59 76 73

L A T I N   A M E R I C A
[email protected]
Brasil
t 55 (11) 3089-7400
Mexico
t (52-81) 8158-8400

Содержание MicroDEN

Страница 1: ...hnical information visit www corning com lifesciences or call 800 492 1110 Outside the United States call 1 978 442 2200 or contact your local Corning sales office Corning Incorporated Life Sciences 8...

Страница 2: ...autoclave this unit Do not perform the sterilization cycle of an incubator with the MicroDEN unit inside Do not use acetone to clean the unit Always use the provided external power supply to power the...

Страница 3: ...nts 1 Corning MicroDEN unit Cat No 431276 requires standard electrical outlet 2 Corning consumable set Cat No 431277 3 Corning storage bottle Cat No 431278 4 Enriched monocytes MOs 5 Differentiation m...

Страница 4: ...t a wall to prevent disturbing the cultured cells The cell culture flasks may be seeded with either enriched MOs or with peripheral blood mononuclear cells PBMCs where the MOs are enriched through pla...

Страница 5: ...ow through the unclamped tube Method 2 Pump Perfusion 1 Unclamp the pumps by pressing upwards at the dotted triangle on the pump head 2 Place the tubing onto the pump rollers The colored tubing marker...

Страница 6: ...emptied on Day 3 1 Turn the Corning MicroDEN unit OFF disconnect the power cord and remove from the incubator 2 Lightly spray the MicroDEN system with 70 ethanol and place inside the biosafety cabine...

Страница 7: ...e new storage bottle containing differentiation medium Disconnect the empty bottle MPCs and connect the new storage bottle MPCs to the inlet side tubing 8 Transfer the Corning MicroDEN system from the...

Страница 8: ...he flasks back onto the thermoform tray with the corner notch of each flask in the upper right position 10 Repeat Steps 1 9 for a second cold buffer wash NOTE Flasks can be harvested individually by u...

Страница 9: ...anol to disinfect before placing in the biosafety cabinet After use the unit can be cleaned with 70 ethanol or 10 bleach solution The unit should be stored upright in a cool dry environment when not i...

Страница 10: ...riginal material or workmanship This warranty does not cover motor brushes fuses light bulbs batteries or damage to paint or finish Claims for transit damage should be filed with the transportation ca...

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