Cleaver Scientific CSL-RVMSCHOICE10, Инструкция по эксплуатации

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15/11/2018 Page 14
Solutions
0.5M EDTA stock (500mL) dissolve in 400 ml distilled water:

93.05g EDTA disodium salt
Fill to 500 ml litre final volume with distilled water
50X TAE stock (1L) dissolve in 750 ml distilled water:

242 g tris base (FW = 121)

57.1 ml glacial acetic acid

100 ml 0.5 M EDTA (pH 8.0).
Fill to 1 litre final volume with distilled water
10X TBE stock (1L) dissolve in 750 ml distilled water:

108 g tris base (FW = 121)

55 g boric acid (FW = 61.8)
 40 ml 0.5 M EDTA (pH 8.0)
Fill to 1 litre final volume with distilled water
Loading Dye
10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue,
and 0.25% xylene cyanole FF in 1x TAE buffer. Only 1–10 ml of the 10x loading
dye should be prepared.
Ethidium Bromide Solution
Add 10 mg of Ethidium Bromide to 1 ml distilled water.
References
1. Sambrook, Fritsch, and Maniatis, Molecular Cloning A Laboratory
Manual,
Second Edition, Cold Spring Harbor Laboratory Press, 1989.
2.
Current Protocols in Molecular Biology,
Greene Publishing Associates
and Wiley-Interscience, 1989.