Cleaver Scientific CSL-RVMSCHOICE10, Инструкция по эксплуатации

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15/11/2018 Page 11
2. Insert the desired length tray into the Flexicaster such that one end of
the tray is pushed up and seals against the silicone mat of the
permanent end of the Flexicaster.
3. Position the movable end of the Flexicaster so that the silicone mat is
pushed against the other end of the tray.
4. Turn the cam so that the silicone mat tightly seals against the side of the
tray. Pour in the agarose carefully so as not to generate bubbles. Any
bubbles that do occur can be smoothed to the edge of the gel and
dispersed using a pipette tip.
5. Allow the agarose to set, ensuring that the gel remains undisturbed.
6. Carefully remove the gel casting gates and comb and transfer the gel
including tray to the main tank.
Tape
1. Autoclave or plastic backed general tape should be used. A length
5cm longer than the width of each end of the tray should be cut. One
length should be placed over one end of the tray and stuck m1cm in
from the tray edge. This should then be folded, and the edges sealed
securely. Repeat for the other end and place onto a level surface for
gel pouring.
2. Place the comb(s) in the grooves. Each tray has more than one comb
grove so that multiple combs can be used. Using multiple combs
increases sample number available per gel but decreases run length
MS7-FC and MS20-FC Flexicasters