Cleaver Scientific CSL-RVMSCHOICE10, Инструкция по эксплуатации

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15/11/2018 Page 12
and care must be taken to ensure that samples from the first wells do
not migrate into the lanes of the second comb wells.
3. Pour in the agarose carefully so as not to generate bubbles. Any
bubbles that do occur can be smoothed to the edge of the gel and
dispersed using a pipette tip.
4. Allow the agarose to set, ensuring that the gel remains undisturbed.
5. Carefully remove the gel casting gates and comb and transfer the gel
including tray to the main tank.
Running the Gel
1. Mix the sample to be loaded with sample buffer or runSAFE 6X DNA
stain for real-time visualisation.
2. Fill the unit with buffer until the gel is just flooded with buffer. This will give
the fastest resolution times. For enhanced quality of resolution of
sample, fill the unit to 5mm above the gel.
3. Load the samples into the wells using pipettes. Multi-channel pipettes
can be used for loading samples with MC compatible combs, see
listing in accessories for identification of these.
4. Carefully place the lid on the tank and connect to the Base Station.
Connect the Lid Fan to the runVIEW Base Station.
5. Typically, gels are run at between 90 and 150 volts. However, maximum
voltages are indicated on the serial badge of each unit. It should be
noted that higher voltages generally give faster but poorer quality
sample resolution.
To operate under constant voltage or constant current modes, adjust the
other parameter to the maximum value. For example, to operate under
constant voltage, adjust the current to the maximum output of 300mA
before running the power supply with the voltage set at the desired output
setting.