Cleaver Scientific CSL-RVMSCHOICE10 страница 1 Скачать руководство пользователя

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runVIEW RealTime

Horizontal System

Содержание CSL-RVMSCHOICE10

Страница 1: ...runVIEW Real Time Horizontal System...

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Страница 3: ...SCHOICE10 CSL RVMSCHOICE15 CSL RVMSCHOCIETRIO Record the following for your records Model _____________________ Catalogue No _____________________ Date of Delivery _____________________ Warranty Perio...

Страница 4: ...he Horizontal Gel Tank 7 Fitting Electrodes 7 Fitting Loading Guides 7 Control Interface 8 Gel Preparation 9 Gel Pouring 10 Casting Dams 10 Flexicaster 10 Tape 11 Running the Gel 12 Stain Compatibilit...

Страница 5: ...e other than properly insulated power supply cables 3 Do not spill liquid or insert metal objects inside the power supply 4 Never block the ventilation holes or place the unit in any enclosure unless...

Страница 6: ...kPa 4 Altitude 2000 metres 5 Operating temperature ambient to 40 C 6 Pollution degree 2 7 Mains supply voltage fluctuations up to 10 of the normal voltage This apparatus is rated POLLUTION DEGREE 2 i...

Страница 7: ..._______________ Date ________________________ The packing lists should be referred to as soon as the units are received to ensure that all components have been included The unit should be checked for...

Страница 8: ...ensions 293 x 220 x 80 mm Operating Mode Constant Voltage or Current Rated Voltage 100 240V 50 60Hz runVIEW Gel System Gel Dimensions W X L 15 X 7 15 x 10 15 X 15cm bluVIEW Lid Design Orange spectral...

Страница 9: ...the Horizontal Gel Tank Fitting Electrodes Note the position of the lid on the unit This shows the correct polarity and the correct orientation of the cables black is negative and red positive Remove...

Страница 10: ...e 2 To switch blue light ON or OFF 3 Increase selected parameter 4 Decrease selected parameter 5 Start stop the program To set the required voltage current and time use the mode button to navigate bet...

Страница 11: ...issolve the agarose powder by heating the agarose either on a magnetic hot plate with stirring bar or in a microwave oven If using the microwave method the microwave should be set at around a 400 watt...

Страница 12: ...n the grooves Each tray has more than one comb grove so that multiple combs can be used Using multiple combs increases sample number available per gel but decreases run length and care must be taken t...

Страница 13: ...llow the agarose to set ensuring that the gel remains undisturbed 6 Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank Tape 1 Autoclave or plastic bac...

Страница 14: ...times For enhanced quality of resolution of sample fill the unit to 5mm above the gel 3 Load the samples into the wells using pipettes Multi channel pipettes can be used for loading samples with MC co...

Страница 15: ...dra Green Safe DNA Dye Higher Intensity Bands Observed HD Green DNA Stain Higher Intensity Bands Observed runSAFE Higher Intensity Bands Observed SafeView DNA Stain Compatible Visible Bands Observed S...

Страница 16: ...base FW 121 55 g boric acid FW 61 8 40 ml 0 5 M EDTA pH 8 0 Fill to 1 litre final volume with distilled water Loading Dye 10x sample buffer stock consists of 50 glycerol 0 25 bromophenol blue and 0 2...

Страница 17: ...Consult Cleaver Scientific about agarose Reduce salt concentration to 0 1M Reduce voltage See electrophoresis instructions Apply sample carefully Increase gel thickness for large sample volumes Adjus...

Страница 18: ...ons to the power supply and within electrophoresis system to make sure the connection is intact check the electrodes to make sure they are intact Close the circuit by reconnecting the cables Press STA...

Страница 19: ...should only be cleaned with the following Warm water with a mild concentration of soap or other mild detergent Compatible detergents include dishwashing liquid Hexane and Aliphatic hydrocarbons The u...

Страница 20: ...10 6 5 22 MS15 12 0 75 Choice 0 75 12 9 30 MS15 14MC 0 75 Choice 0 75 14 6 5 22 MS15 16 0 75 Choice 0 75 16 6 20 MS15 20 0 75 Choice 0 75 20 4 75 16 MS15 28MC 0 75 Choice 0 75 28 2 5 8 MS15 35 0 75 C...

Страница 21: ...e 2 1 110 990 MS15 2 2 Choice 2 2 50 450 MS15 4 2 Choice 2 4 27 243 MS15 10 2 Choice 2 10 10 90 MS15 10MC 2 Choice 2 10 6 5 59 MS15 12 2 Choice 2 12 9 81 MS15 14MC 2 Choice 2 14 6 5 59 MS15 16 2 Choic...

Страница 22: ...5g tablets CSL RUNSAFE CSL RUNSAFE Package 1 ml vial CSL TBEP Powdered Tris Borate EDTA Running Buffer 10 sachets 1litre pk TBE10X1L Cleaver Buffer Tris Borate EDTA Running Buffer 10 x 1L TBE10X5 Cle...

Страница 23: ...e other than CSL or an appointed distributor or representative are no longer under warranty from the time the unit was modified Units which have accessories or repaired parts not supplied by CSL or it...

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