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The algorithm performs baseline subtraction on background subtracted values. Background
subtracted values are obtained after the algorithm adjusts for well-to-well variations and
deconvolution (for dual channels only) on the amplification data.
Baseline subtraction is turned on by default. This can be turned off by toggling the Baseline
Subtraction option on the top left of the amplification plot. Please note that Cq values are
calculated from baseline-subtracted values. This means that the Cq values will remain the
same regardless of whether baseline is turned on or off.
Figure 5.4 displays the results screen of a completed sample run in a dual channel cycler. To
view the exact Cq and Relative Fluorescence Units (RFU) for a specific cycle, click on one of the
curves (Figure 5.5).
Figure 5.4
Figure 5.5
SAMPLE NAME INPUT
Sample names may be entered in the software post-run for tracking purposes. Once the run
has completed, select each box under the
Sample Name
column and type in the sample name
as appropriate. The names will be automatically saved once entered into each row (Figure 5.6).
Figure 5.6
GRAPH DISPLAY FUNCTIONS
Select/Deselect samples
Drag to select all adjacent wells simultaneously or select noncontiguous wells to view curves
from specific samples. To view data from noncontiguous wells, click on each well while holding
down the Command key (for Mac users) or the Control key (for PC users).
