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ASX-1400 Autosampler Operator’s Manual

Installing the Autosampler

3–15

operation of the ASX-1400 Autosampler. The ASX-1400 Autosampler
supports the following two communications protocols:



The serial (RS-232) protocol is the standard configuration. There are
two RS-232 serial ports on the ASX-1400, and a serial interface kit is
shipped with the autosampler.



The parallel (IEEE-488) protocol is less common than the serial
configuration. An IEEE-488 interface kit is available as an optional
accessory to the ASX-1400. See Chapter 1, “Introduction,” for more
information about this kit.



The USB interface is an optional configuration. A virtual COM port
is created when using the USB and therefore, the connection looks
like a standard RS-232 serial port to the host PC software.

Note:

Although the ASX-1400 supports serial and IEEE communications

protocols, the host computer governs which protocol is used. To

determine which protocol is required for the analytical instrument you are

using, refer to the hardware or software manual provided with the

instrument. The following sections explain how to establish a

communications interface using a serial protocol and using an IEEE

protocol.

When  interconnecting  any  computing  devices,  keep  the
communications cables away from sources of electromagnetic or
Radio  Frequency  (RF)  interference,  such  as  electric  motors,
transformers, fluorescent light ballasts or RF energy sources.

Establishing an RS-232 Serial
Communications Interface

The serial interface kit provided with the ASX-1400 Autosampler
includes an interface cable equipped with two modular port adapters.
Use the interface kit to establish a serial communications interface with
the host computer. To do so, complete the following steps:

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Содержание ASX-1400

Страница 1: ...ASX 1400 Autosampler Operator s Manual ...

Страница 2: ...of CETAC the warranty will be voided In addition this warranty does not extend to repairs made necessary by the use of parts accessories or fluids which are either incompatible with the unit or adversely affect its operation performance or durability CETAC s obligation under this warranty is strictly and exclusively limited to repair or replacement of defective CETAC parts and no claim of breach o...

Страница 3: ...eturned later than thirty 30 days after issuance of a return authorization number will be subject to review Shipment of repaired products will generally be made for ty eight 48 hours after the receipt Products may not be returned which are contaminated by radioactive materials infectious agents or other materials constituting health hazards to CETAC employees Returned Product Warranty Determinatio...

Страница 4: ...y are published SAFETY Instruments accessories components or other associated materials may not be returned to CETAC Technologies if contaminated with biohazard or radioactive materials infectious agents or any other materials and or conditions that could constitute a health or injury hazard to CETAC employees Call Customer Service and Support if there is any question or doubt relative to decontam...

Страница 5: ...400 Operator s Manual and ensuring that it is operated safely and properly ORIGINAL PACKAGING Retain original factory packaging for moves and factory return shipments Shipping in anything other than the original fitted foam and container can result in incidental damage from which the purchaser will not be protected under warranty Under all conditions the user must observe safe laboratory procedure...

Страница 6: ...interference at his own expense MODIFICATIONS The FCC requires the user to be notified that any changes or modifications made to this device that are not expressly approved by CETAC may void the user s authority to operate the equipment CABLES Connections to this device must be made with shielded cables with metallic RFI EMI connector hoods to maintain compliance with FCC Rules and Regulations CAN...

Страница 7: ...ectrical shock do not open the cabinet All maintenance is to be performed by an Authorized CETAC Service Provider Protection provided by the equipment may be impaired if the equipment is used in a manner not specified by the manufacturer CLEANING INSTRUCTIONS To clean the exterior surfaces of the instrument complete the following steps 1 Shut down and unplug the instrument 2 Wipe the instrument ex...

Страница 8: ...RE ENLEVE QUE PAR UN RÉPARATEUR QUALIFIÉ AVERTISSEMENT TOUT CONTACT AVEC LES HAUTES TENSIONS PEUT ENTRAINER LA MORT OU DES BLESSURES SÉVÈRES CE PANNEAU NE DOIT ÊTRE ENLEVE QUE PAR UN RÉPARATEUR QUALIFIÉ AVERTISSEMENT TOUT CONTACT AVEC LES HAUTES TENSIONS PEUT ENTRAINER LA MORT OU DES BLESSURES SÉVÈRES CE PANNEAU NE DOIT ÊTRE ENLEVE QUE PAR UN RÉPARATEUR QUALIFIÉ AVERTISSEMENT TOUT CONTACT AVEC LES...

Страница 9: ...Technologies the protection provided the equipment may be impaired Crush Hazard Pinch Point Keep hands clear of moving parts X Y Z axis movement may crush hand Puncture Hazard Moving parts can cause severe injury Do not put hand under the probe assembly Lifting Hazard Single person lift could cause injury Use assistance when moving or lifting Attention Refer to the manual This symbol indicates tha...

Страница 10: ...d incorporated in the power cord The mains plug shall only be inserted in a socket outlet provided with a protective earth contact Intentional interruption is prohibited Safety Maintenance The operator should check the detachable power supply cord condition The equipment should not be operated if the mains inlet is cracked or broken Any obvious damage to the case from a drop or fall should be chec...

Страница 11: ...the event a supplier cannot be reached contact CETAC Technologies customer service department at 1 800 369 2822 WARNING Pollution Degree 1 WARNING Equipment is not intended for wet locations Miscellaneous liquids in the equipment could cause hazardous conditions WARNING Ensure that power is disconnected before removal of any covers WARNING All external I O terminals allow a maximum current of 150 ...

Страница 12: ...Contents ...

Страница 13: ...enu Items xiv Terminology xiv Notes xv Cautions xv Warnings xv Where to Go for More Information xvi 1 Introduction 1 2 Autosampler Standard Components 1 2 Optional Accessories 1 6 2 Preparing for Installation 2 2 Choosing a Location 2 2 Space Requirements 2 2 Water Requirements 2 2 Power Requirements 2 3 Unpacking the ASX 1400 2 4 ...

Страница 14: ...inse Station 3 6 Pumped Drain Arrangement 3 7 Changing the Rinse Pump Speed 3 11 Assembling and Placing the Sample Vial Racks 3 11 Establishing External Connections 3 13 Connecting the Autosampler to the Power Source 3 13 Connecting the Autosampler to an Analytical Instrument 3 13 Connecting the ASX 1400 Autosampler to the Host Computer 3 14 Establishing a Serial Communications Interface 3 15 Esta...

Страница 15: ...s 5 2 Creating the Lab Environment 5 3 Replacing Autosampler Components 5 4 Purchasing Supplies 5 4 Arranging the Sample Vial Racks 5 5 Starting the Autosampler 5 5 Shutting Down the Autosampler 5 7 Flushing the Rinse Station and Flow Path 5 7 6 Maintaining the Autosampler 6 2 Cleaning the Autosampler 6 2 Daily External Cleaning 6 3 Weekly Cleaning 6 3 Checking for Leaks 6 5 Replacing Peristaltic ...

Страница 16: ...tosampler 7 2 Power System Problems 7 2 Interface Problems 7 3 RS 232 Cable Problems 7 3 Software Configuration Problems 7 3 Z Drive Assembly Problems 7 4 8 Appendix A 8 2 Dual 2 Channel Peristaltic Pump 8 2 Connecting the Rinse Station 8 2 Pumped Drain Arrangement 8 2 Gravity Drain Arrangement 8 2 Changing the Rinse Pump Speed 8 2 Glossary G 2 ...

Страница 17: ...Preface ...

Страница 18: ...ter experience and working knowledge of the analytical instrument used with the ASX 1400 How to Use This Book The ASX 1400 Autosampler Operator s Manual contains seven chapters You should read the chapters sequentially the first time Thereafter refer to the chapters separately as needed The first chapter provides an introduction to the autosampler Subsequent chapters detail the primary tasks assoc...

Страница 19: ...you perform during daily operation of the ASX 1400 Chapter 6 Maintaining the Autosampler explains daily weekly and periodic maintenance tasks Chapter 7 Troubleshooting the Autosampler describes how to diagnose and correct ASX 1400 problems These chapters are followed by a glossary of related terms Conventions Used in This Book This book uses certain conventions to distinguish different types of in...

Страница 20: ...enu choice in the Settings Menu Terminology This book frequently uses the following terms Host Computer The computer that controls operation of the ICP AES or ICP MS instrument to which the autosampler is attached Hz Hertz ICP AES An inductively coupled plasma atomic emission spectrometer ICP MS An inductively coupled plasma mass spectrometer ID Inside diameter LED Light emitting diode PEEK Polyet...

Страница 21: ... are indicated as follows Note This example shows how a note is displayed Cautions Cautions indicate situations that require immediate attention to prevent harm to the autosampler Cautions are indicated as follows This example shows how a caution is displayed Warnings Warnings indicate situations that could cause bodily harm Warnings are indicated as follows This example shows how a warning is dis...

Страница 22: ...addition to the ASX 1400 Autosampler Operator s Manual you can refer to the following resources The software manual for the ICP AES or ICP MS instrument you are using CETAC Technologies Customer Service and Support 1 800 369 2822 1 402 733 2829 1 402 733 1932 Fax E mail custserv cetac com ...

Страница 23: ...1 Introduction ...

Страница 24: ...sampler Standard Components Autosampler components are made of corrosion resistant stainless steel alloys or anodized aluminum The enclosure and base are made from a high strength aluminum alloy that is chromated and finished with an epoxy powder coating The ASX 1400 operates reliably under a wide variety of conditions Components in the sample flow path are made of Polyetherimide PEI and Polytetra...

Страница 25: ...ause failure of the PEI flow path components Solutions of concentrated acids greater than 40 Solutions of concentrated bases greater than 10 potassium ammonium or sodium hydroxides Partially halogenated hydrocarbons or extremely aggressive organic solvents chloroform methylene dichloride 1 1 2 trichloroethane Figure 1 1 ASX 1400 Autosampler Front View CAUTION ...

Страница 26: ...dle five sets of Gilson 60 position number 29 rack c Hold three of the APS 1650 80 position collection racks 3 Standards Vials Ten standards vials are included with the autosampler The standards vials which fit into the standards positions at the back of the sample tray are 50 milliliter conical centrifuge vials with caps 4 Flowing Rinse Station The rinse station is located in the middle of the st...

Страница 27: ...el peristaltic pumps The ASX 1400 depending upon the model purchased will be standard with one on board peristaltic pump The pump is located in the lower right hand corner on the back of the autosampler The pump moves the rinse solutions from the rinse source through the flowing rinse stations Previous versions of the ASX 1400 shipped with two two channel peristaltic pumps One main pump and one au...

Страница 28: ...ob can either be set at a factory set speed or control the stirrer speed to optimize mixing of the sample 6 Pump Speed Control The control knobs can either be set at a factory set pump speed or control the pump speed to optimize rinse flow There is two control knobs to control the main and auxiliary pumps The following standard components are also shipped with the ASX 1400 External Desktop Power S...

Страница 29: ...erter box an IEEE 488 cable a power cord and instructions It is used to convert an IEEE communications protocol to a serial protocol Serial Interface Null Adapter The null adapter replaces one DB9F port adapter at the host computer It is used for computers with DCE AT style serial ports Serial Interface Special Adapter Kit The adapter kit replaces one or both standard serial port adapters with unw...

Страница 30: ...ptum needle No markings Screened SS probe No markings Note Contact CETAC Technologies if you need additional accessories not listed need added features to integrate the ASX 1400 Autosampler into your analytical system or have unique requirements Research and development of new features and accessories for the ASX 1400 Autosampler often inspired by customer requests is a continuing activity at CETA...

Страница 31: ...2 Preparing for Installation ...

Страница 32: ...nvironment for the availability of space water and power For the autosampler to function optimally the location you select must meet specific requirements associated with each of these items The following sections discuss space water and power requirements Space Requirements Most analytical applications benefit from the shortest sample flow path Therefore you should place the autosampler close to ...

Страница 33: ... of the autosampler below the power switch Connect the power supply to the autosampler first and then connect a line cord to the power supply Do not apply power to the power supply until ready to operate the autosampler The ASX 1400 is intended to operate from an AC power source that will not apply more than 240V AC between the supply conductors and ground A protective ground connection by way of ...

Страница 34: ...sampler allow it to dry thoroughly before connecting it to a power source and operating it Failure to do so may cause equipment damage Remove the packing checklist from the shipping container and check off items against it Leave accessories in the packing until you are ready to install them on the autosampler Note Do not throw away the factory packaging Keep it for possible future use This is one ...

Страница 35: ...3 Installing the Autosampler ...

Страница 36: ...en them with anything other than your fingers To install the autosampler you must complete the following tasks Each of these tasks will be discussed in detail later in this chapter 1 Removal of carriage shipping fixture 2 Mounting the Z Drive assembly 3 Connect the rinse station 4 Assemble the sample vial racks 5 Establish external connections 6 Connect the autosampler to the host computer The ASX...

Страница 37: ...w in the same location Keep the shipping fixture with the packaging materials for future use Figure 3 1 Shipping Fixture Mounting the Z Drive Assembly Mounting the Z Drive assembly on the autosampler is the first task The Z Drive assembly must be attached to the autosampler arm to allow movement and function of the sample probe Figure 3 1 illustrates the Z Drive assembly components Attaching the Z...

Страница 38: ... the pins in the corresponding holes in the mounting block while holding it stable 5 Insert the screws one at a time while continuing to hold the assembly stable 6 Be careful not to tighten the screws too much Note The screws are going into plastic and the plastic threads can be easily stripped if the screw is over tightened ...

Страница 39: ...ASX 1400 Autosampler Operator s Manual Installing the Autosampler 3 5 Figure 3 2 Mounting of Z Drive Assembly ...

Страница 40: ...nd Removal of the Sample Probe 1 To remove the sample probe loosen the thumbscrew and pull probe straight out 2 To install the sample probe simply reverse the directions for removal When installing make sure the bottom of the sample probe is even with the end of the stirring paddle Removal of Stirring Assembly 1 With the power off manually push the stirring assembly of the way down 2 Loosen the th...

Страница 41: ...tion from the kerosene family are used as the rinse solution and is pumped into the rinse station by the on board 3 channel peristaltic pump There is one 3 channel pump on the ASX 1400 Autosampler one channel is used to supply rinse solution to the rinse station for the stirring paddle and sample probe while the other two channels are used to pull rinse solution from the rinse station reducing the...

Страница 42: ... out the top of the rinse station into the drain reservoir The drain reservoir is pumped by the front and rear channels of the on board peristaltic pump as shown in Figure 3 2 There are many different types of tubing that can be used for the rinse station Viton is the pump tubing that ships with each unit Tygon Fuel and Lube yellow Tygon clear and PharMed opaque can also be used for pump tubing de...

Страница 43: ...ASX 1400 Autosampler Operator s Manual Installing the Autosampler 3 9 Figure 3 2 Pumped Drain Arrangement ...

Страница 44: ... rinse solution uptake 2 Connect the peristaltic pump to the rinse station by connecting the 23 inch length Superthane tubing to the bottom center channel of the Viton pump tubing The rinse station snaps into the chassis and is secured by a screw It must be removed to attach the rinse tubing a Insert the other end of the 23 inch length of Superthane tubing through the channel at the bottom of the ...

Страница 45: ...r ends to the top rear channel and top front channel of the Viton pump tubing f Connect two 3 1 2 inch lengths of Superthane tubing to the bottom front and bottom rear channels of the Viton pump tubing g Connect the other ends to the Y connector The drain rinse out channel is combined into a single channel for your waste reservoir h Connect the 84 inch length of Superthane tubing to the remaining ...

Страница 46: ...me versions of sample vial racks are shipped unassembled However you can easily assemble them without using tools Once you assemble the sample vial racks place them in the sample tray before proceeding with the installation Before loading or unloading any sample vial racks on the sample tray park the sampling arm and probe in the home position by cycling the power on and off The home position is t...

Страница 47: ...e than 2mm in either a left right or forward backward direction unless you lift them 3 Place the next sample vial rack to the right of the previous rack so the feet on the rack s underside engage the locating ribs on the sample tray s surface Ensure the rack is not tilted and is properly placed 4 Repeat step three until you place all the sample vial racks When viewed from the front of the ASX 1400...

Страница 48: ...el of the autosampler Then plug the power supply s power cord into a 100 240 VAC 10 50 60Hz utility power outlet Connecting the ASX 1400 to an Analytical Instrument You can connect the autosampler directly to a sample introduction peristaltic pump and then to any sample introduction device such as the CETAC U 5000AT or U6000AT Ultrasonic Nebulizer To do so complete the following steps 1 Determine ...

Страница 49: ...ware Note Although the ASX 1400 supports serial and IEEE communications protocols the host computer governs which protocol is used To determine which protocol is required for the analytical instrument you are using refer to the hardware or software manual provided with the instrument The following sections explain how to establish a communications interface using a serial protocol and using an IEE...

Страница 50: ...M No not use a null modem adapter 3 Connect the other end of the cable to the ASX 1400 COM1 port Ensure you are connecting the adapter to the COM1 port Connecting the adapter to the dilutor port on the autosampler will cause a malfunction The autosampler dilutor port is used for communications to the autodilutor or devices other than the host computer 4 Finger tighten both screws of the capable ad...

Страница 51: ... Finger tighten both screws of the cable adapter Establishing a USB Communications Interface The serial interface kit provided with the ASX 1400 Autosampler does not include a USB cable This cable may be obtained from any computer store Complete the following steps for the USB interface 1 Power up both the computer and the autosampler 2 Plug one end of the cable into the host computer s USB port a...

Страница 52: ...4 Verifying Installation ...

Страница 53: ... communications interface between it and the host computer is working Ensuring that the sample probe functions properly This chapter explains how to test the above items before using the autosampler Note The procedures given in this chapter are for use in a Windows 95 or Windows NT environment Testing the Interface If the communications interface between the autosampler and the host computer is no...

Страница 54: ...llowing steps 1 Start the host computer and go to the main Windows screen 2 Turn on power to the autosampler 3 Click the start button in the lower left corner of the Program Manager window a A selection list will appear 4 Select Programs Accessories Communications Hyperterminal and double click a The Hyperterminal 1 window appears Figure 4 1 Figure 4 1 Example of Hyperterminal Window 1 Hypertermin...

Страница 55: ...5 Double click on the Hyperterminal icon a The Connection Description box appears Figure 4 2 Figure 4 2 Connection Description Box 6 Type the name COM1_test for the connection and choose an icon from the list given and click OK The phone number box appears Figure 4 3 ...

Страница 56: ...osampler Operator s Manual Verifying Installation 4 5 Figure 4 3 Phone Number Box 7 Select Direct to COM1 in the Connect Using box Click OK The COM1 Properties box appears Figure 4 4 Figure 4 4 COM1 Properties Box ...

Страница 57: ...ct File Properties The COM1_Test Properties box appears 10 Select Settings on the COM_Test Properties box and click on the ASCII setup button on the lower right of the box The ASCII setup box appears Figure 4 5 Figure 4 5 ASCII Setup Box 11 In the ASCII Setup box select the following items a Echo typed characters locally b Append line feeds to incoming line ends c Wrap lines that exceed terminal w...

Страница 58: ...ponents for damage before you operate the autosampler To do so complete the following steps 1 Shut down and unplug the autosampler 2 Visually inspect the sample probe peristaltic pump tubing and rinse station and tubing for leaks or signs of damage If you detect a leak or other damage to an autosampler component you must replace it For more information see the appropriate section in Chapter 6 Main...

Страница 59: ... Autosampler 2 Turn the autosampler power switch on and verify that the LED power indicator is on The LED power indicator is green in color The indicator is located behind the Z Drive assembly when it is in the home position 3 Using the host computer control software designate the sample positions at the left rear left front right rear and right front of the sample tray 4 Place sample vial racks a...

Страница 60: ...5 Using the Autosampler ...

Страница 61: ...sing the ASX 1400 Autosampler It also explains how to arrange the sample vial racks start and shut down the autosampler and flush the rinse station and flow path Establishing Optimal Operating Conditions The ASX 1400 Autosampler operates reliably even under less than ideal conditions It is not however indestructible Malfunction or damage can occur if specific operating conditions are not met Meeti...

Страница 62: ...ids or large spills into the autosampler cabinet or arm Exposures of this type can damage the drive mechanisms as well as the electronics Observe the same general electrostatic discharge precautions as with any other integrated circuit electronic devices Low humidity environments especially when combined with static generating materials require maximum care Discharge static buildup and ground to t...

Страница 63: ... you should maintain an adequate supply of spare vials When you need to purchase additional supplies it is extremely important that you choose the appropriate sizes and materials When you purchase sample and or standards vials make sure they meet the following requirements The diameter of the sample or standards vial matches the rack size you are using The diameter of the standards vial is 28 mill...

Страница 64: ...all size combinations and arrangements of sample vial racks However you can use racks of mixed sizes only if the host computer s software enables you to do so For more information about placing sample vial racks in the sample tray see Chapter 3 Installing the Autosampler Incorrectly defining the position count can result in sample spills and invalid analysis results Starting the Autosampler Once y...

Страница 65: ...ubing in the rinse solution source and running the rinse solution through the rinse station Ensure there are no air bubbles visible in the rinse uptake tubing before you run samples with the ASX 1400 Autosampler Note If you are flushing the rinse system during initial startup first use a 2 nitric acid solution as the rinse agent Flush the rinse system a second time using deionized water as the rin...

Страница 66: ...he pressure shoe on the peristaltic pump Releasing the pressure shoe decreases wear on the pump tubing 3 Turn off the ASX 1400 Autosampler power switch Flushing the Rinse Station and Flow Path Generally you can operate the ASX 1400 Autosampler without flushing the rinse system Under normal circumstances you can simply drain the rinse system prior to shutting down the autosampler However you need t...

Страница 67: ...ial startup first use a 2 nitric acid solution as the rinse agent followed by deionized water 2 Run the rinse solution through the rinse station and flow path for 5 to 10 minutes Once you flush the rinse system you can proceed with the sampling sequence or drain the rinse system as part of the shutdown procedure For information about running the sampling sequence see Starting the Autosampler earli...

Страница 68: ...6 1 6 Maintaining the Autosampler ...

Страница 69: ...components Discharge static buildup and ground to the autosampler base or cabinet before performing any maintenance Do not touch or short circuit bare contacts COM1 Dilutor or auxiliary ports Cleaning the Autosampler Cleaning the ASX 1400 Autosampler is the primary maintenance task you perform Failure to do so regularly causes increased wear and reduces the autosampler s life You must clean the AS...

Страница 70: ... grade cleaning agent Do not allow the cleaning agent to come into contact with the lead screws Also never lubricate either of the two lead screws 3 Repeat step two using a towel dampened with clear water This process removes any remaining contaminants 4 Dry the sample tray autosampler cabinet and autosampler arm using a dry towel The ASX 1400 Autosampler must be thoroughly dry before you turn the...

Страница 71: ...e sample tray see Replacing the Sample Tray later in this chapter 3 Wipe loose particles off the Y Axis lead screw with a dry lint free cloth The Y Axis lead screw is a large metal screw located inside the Autosampler arm tubing as shown in Figure 6 1 Figure 6 1 Z Drive Assembly on the Autosampler Arm ...

Страница 72: ...ains 6 Rinse the sample tray with water and then dry it Ensure that the sample tray is thoroughly dry 7 Replace the sample tray on the autosampler base For information about replacing the tray see Replacing the Sample Tray later in this chapter Checking for Leaks Several of the autosampler components have a limited life and will wear out under normal use the sample probe the peristaltic pump tubin...

Страница 73: ...olvents as rinsing agents the tubing may break down rapidly To replace the peristaltic pump tubing complete the following steps 1 Shut down and unplug the autosampler 2 Release the pressure shoe and remove the old tubing Carefully pull or cut the old tubing to remove it 3 Replace the pump tubing by pushing the new tubing onto the mounting block fittings Replace the new tubing carefully Damage can ...

Страница 74: ...s the tubing is likely to deteriorate more rapidly To replace the rinse station tubing complete the following steps 1 Shut down and unplug the autosampler 2 Move the autosampler arm 20 to 30 centimeters away from the home position by gently pushing it Moving the autosampler arm ensures that the sample probe will not be damaged while you replace the rinse station tubing 3 Disconnect the rinse solut...

Страница 75: ...tion tube into the mounting block and rotating it clockwise 1 4 turn 4 Reconnect the rinse solution uptake and drain tubing Apply only a linear force when replacing the tubing to prevent the fittings from breaking 5 Move the autosampler arm back to the home position ...

Страница 76: ...7 Troubleshooting the Autosampler ...

Страница 77: ...not solve a problem using the steps given in this chapter contact CETAC Technologies Customer Service and Support Power System Problems A possible cause of ASX 1400 Autosampler malfunction is a problem in the power system If the autosampler is not functional there may be no power getting to it If this is the case the green LED power indicator will be off To troubleshoot this problem complete the f...

Страница 78: ...onnected to the appropriate COM port If the cable is plugged in ensure that it is tightened properly For more information about connecting the RS 232 cable see Chapter 3 Installing the Autosampler Software Configuration Problems If the RS 232 cable is connected properly and the autosampler is still not communicating with the host computer ensure that the host software is configured correctly To do...

Страница 79: ... Chapter 3 Installing the Autosampler to install it If the Z Drive assembly is already installed continue with step two 2 Check the Y Axis position magnet for damage The home position magnet is shown in Figure 7 1 If the magnet is damaged or blocked you must replace or clean the assembly For information about mounting the Y Axis slider block on the autosampler are see Chapter 3 Installing the Auto...

Страница 80: ...ASX 1400 Autosampler Operator s Manual Troubleshooting the Autosampler 7 5 Figure 7 1 Z Drive Assembly with Y Axis Block Home Position Flag ...

Страница 81: ...8 Appendix A ...

Страница 82: ...on board main 2 channel peristaltic pump plus one Auxiliary 2 channel peristaltic pump If your ASX 1400 contains dual peristaltic pumps refer to these instructions If your ASX 1400 contains a single 3 channel peristaltic pump refer to the instructions starting on 3 6 Figure 1 1 ASX 1400 Autosampler Back View ...

Страница 83: ...mple probe tube between samples Reversing the connections and the rinse solution flow reduces the effectiveness of the rinse station and can cause cross contamination and unsatisfactory performance The waste rinse solution drains from the top of the rinse station by means of a pumped drain which is the standard arrangement for draining the rinse station If it is not desirable to use the pumped dra...

Страница 84: ...ached to the back of the instrument above the other pump There are holes keyed onto the back of the pump and correspond with the plastic pieces attached to the instrument Plug the auxiliary cable into the plug on the back of the instrument next to the pump Figure 3 2 Pumped Drain Arrangement To connect the rinse station using the pumped drain complete the following steps ...

Страница 85: ...tubing carefully because the peristaltic pump fitting grips the tubing tightly If you apply too much force the fitting can break off b Insert the other end of each 3mm i d Tygon tubing onto the rinse tubes at the bottom of the rinse station Again insert the tubing to avoid breaking the fitting 3 Connect the rinse station to the auxiliary pump of the on board peristaltic pump by completing the foll...

Страница 86: ...ovided for the pumped drain a Insert the two pieces of the 3mm i d Tygon tubing onto the auxiliary peristaltic pump outlet Insert the tubing carefully because the rinse station fitting grips the tubing tightly If you apply too much force the fitting can break off b Place the other ends of the tubing into the waste container Ensure that the tubing outlet is placed in the waste container so that it ...

Страница 87: ...eans of a gravity drain The main pump is used to supply rinse solution to the rinse station The auxiliary pump is not used To connect the rinse station using a gravity drain complete the following steps 1 Connect the rinse solution to the main on board peristaltic pump by inserting the 3mm milimeter i d Tygon tubing onto the inlet at the top of the pump 2 Connect the peristaltic pump to the rinse ...

Страница 88: ...too much force the fitting can break off 3 Connect the rinse station to the waste container by completing the following steps Use up to 1 8 meters of the i d Tygon tubing provided for the gravity drain Ensure the waste container is at least 30cm to 60cm lower than the rinse station outlet a Insert the 3mm i d Tygon onto the rinse station outlet on top Insert the tubing carefully because the rinse ...

Страница 89: ... are black knobs located on the rear of the unit that control the speed of the pumps One knob controls the stirrer and the other two control the pumps If you turn the knobs all the way to the left counter clockwise you will hear a click When you hear that the pumps are set at the factory settings for speed ...

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