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6

1. Safety information

Before you start using BIO X, please take time to review the entire manual and the 

manuals for any accessories. Mishandling of  BIO X can lead to severe and irreversible 

injuries if  the safety instructions are not followed. Throughout the manual we will 

use the following symbols to indicate if  there is risk of  personal injury or equipment 

damage:

CELLINK AB

Arvid Wallgrens Backe 20

Gothenburg, SE 41346

SWEDEN

1.1 General safety information

In order to respond to BIO X and its relevant security requirements, all personnel using 

BIO X must have read through the entire manual. Should anything appear out of  order 

or a situation occur that is not described in the manual, contact CELLINK before using 

the instrument any further!

Use the instrument only for its intended purpose as described in the documentation. Do 

not modify the instrument, sub-components or accessories. Do not open or disassemble 

BIO X or attempt any service beyond the maintenance instructions provided in the 

manual. BIO X and the CELLINK intelligent printheads are only to be used with each 

other, not with other equipment.

This symbol indicates that there is risk of  personal injury or equipment 

damage due to harmful UV radiation in BIO X. Always keep the door 

of  BIO X closed and wear proper safety equipment when using the UV 

sterilization or UV curing systems. This symbol will also be visible on BIO 

X’s touch screen at any time a UV LED is active.

Caution! Never reach into the instrument until all components have come 

to a complete stop. Moving parts can cause injury.

This symbol indicates that there is a general risk of  personal injury or 

equipment damage due to reasons not related to UV radiation or heated 

surfaces.

Caution! Before cleaning or servicing BIO X, turn off  the power and 

disconnect the instrument from any power sources and external air pressure 

sources.

This symbol indicates that there are surfaces in BIO X that are heated and 

might cause personal injury or material damage if  not handled with care. 

Beyond the manual, the physical surface with a heating function will be 

marked with this symbol in BIO X. This symbol will also be visible on BIO X’s

touch screen at any time a surface in the printchamber is heated.

Manufacturer

Содержание BIO X 3D Bioprinter

Страница 1: ...User Manual BIO X 3D Bioprinter...

Страница 2: ...ivalled usability and versatility BIO X will help in taking your research to the next level With features such as interchangeable intelligent printheads clean chambertechnology printchamber sterilizat...

Страница 3: ...X 16 3 2 Contents of the box 17 3 3 Setting up BIO X 19 3 4 Starting BIO X for the first time 20 4 Bioprinting with BIO X 22 4 1 Sterilizing using UV light 23 4 2 Bioprinting from a 3D model 24 4 3 B...

Страница 4: ...ISO 9001...

Страница 5: ......

Страница 6: ...and the CELLINK intelligent printheads are only to be used with each other not with other equipment This symbol indicates that there is risk of personal injury or equipment damage due to harmful UV ra...

Страница 7: ...is a heavy instrument Always have a minimum of two people when unpacking or moving BIO X Always lift and carry the instrument in the manner described in the manual Please read through the instruction...

Страница 8: ...for any warranty condition representation or term the extent to which cannot be excluded or limited by applicable law CELLINK and its suppliers make no warranty condition representation or term expres...

Страница 9: ...9...

Страница 10: ...Specifications...

Страница 11: ...2b 1 Clean Chamber tm unit 2a UV sterilization LED 1 2b UV sterilization LED 2 3a Printhead mount 1 3b Printhead mount 2 3c Printhead mount 3 4a Photo curing module mount 1 4b Photo curing module moun...

Страница 12: ...12 2 1 Product map 5 6 7 2 1a 1b 3 4 1a 1b 2 3 4 5 6 7 Heat vent from the printbed Heat vent from the printbed Power switch Fuse Power socket USB port Ethernet port Pneumatic port...

Страница 13: ...13 2 2 Technical specifications Power input 100 240 VAC 50 60 Hz 600 W Fuse 250V T8A Air pressure input 0 700 kPa Weight 17 kg Height 500 mm Width 360 mm Depth 450 mm Filter class HEPA 14...

Страница 14: ...14...

Страница 15: ...Getting started...

Страница 16: ...y table and a floor space of at least 3 x 2 meters 2 Place the box containing BIO X on its side in accordance to the arrows on the side of the box 3 Carefully remove the tape on the top of the box 4 U...

Страница 17: ...sheet and Application note 5x 3mL CELLINK GelMa bioink sterile 1x Material safety data sheet and Application note 2x 3mL CELLINK START bioink sterile 1x Material safety data sheet and Application not...

Страница 18: ...recision conical nozzles 50 pcs Blue GA 22 1x Sterile high precision conical nozzles 50 pcs Red GA 25 1x Sterile high precision conical nozzles 50 pcs Clear GA 27 1x Sterile blunt needles 20G 50 pcs 1...

Страница 19: ...eads If you wish to use a higher pressure than this connect an external air supply to the back of BIO X see 2 1 Product map Next switch on the instrument using the power switch on the back see 2 1 Pro...

Страница 20: ...ar Symbols in this area will alert you of potential hazards such as hot surfaces or active UV lights see the symbols below Use caution when these symbols are displayed 3 Bioprint Tap to start a 3D Bio...

Страница 21: ......

Страница 22: ...Bioprinting...

Страница 23: ...vironment BIO X is equipped with a UV sterilization system which will create a sterile printchamber which is then maintained sterile by the Clean Chamber Technology CELLINK recommends running the UV s...

Страница 24: ...remain connected to BIO X until the bioprinting process has begun Note The USB Drive needs to be read and writeable Next choose the model you wish to bioprint BIO X accepts the file formats stl obj a...

Страница 25: ...at you want to print on and the size of the surface 1 2 3 1 Petri dish Select to bioprint on a petri dish This is a versatile option useful for many different applications 2 Well plate Select to biopr...

Страница 26: ...to toggle between the settings for the different printheads and the printbed 2 Toggle printhead Tap to select if you want to use the printhead or not If you select to use the printhead further settin...

Страница 27: ...printhead parameters change depending on what type of printhead you decide to use 1 Nozzle type Tap to select what type and size of nozzle you want to use 2 Printhead settings These settings change d...

Страница 28: ...ed You can choose between having the printbed at a certain temperature or to turn the temperature control off and bioprint at the ambient temperature 1 Clean chamber fan Toggle the clean chamber fan o...

Страница 29: ...sen layer profile can be seen here 2 Preview slider Drag this to select which layer to preview 3 Infill pattern Tap to choose infill pattern 4 Printhead use Tap to select which part of the model each...

Страница 30: ...nd compression Linear pattern The linear pattern is suitable as an infill for tissue constructs that must process anisotropic mechanical characteristics Tissues such as ligament tendon muscle and nerv...

Страница 31: ...rint a support structure for your 3D model Use this option if you are planning to bioprint a structure which cannot support itself 4 2 Bioprinting from a 3D model Layer settings Printhead use In the P...

Страница 32: ...incorrect you can easily go back to that step and correct it by pressing the navigation bar at the bottom of the screen 1 4 2 5 3 6 1 Printhead 1 Settings for printhead 1 2 Printhead 2 Settings for pr...

Страница 33: ...d print surface in BIO X By following your settings on the screen it is easy to set up BIO X correctly for your bioprinting process Remember not to start the bioprinting process before you have correc...

Страница 34: ...will start When inserting your print surface use the spring loaded arms to securely fasten it according to the figure below If you are using a well plate simply turn the arms to the sides Note If you...

Страница 35: ...printhead 3 4 Surface probe calibration tab for the surface probe 5 Enable ABL Toggle the ABL option on off The ABL should be enabled after calibrating the surface probe and going back to the Printhea...

Страница 36: ...t UV curing module Allows you to monitor the specific properties of the left UV curing module 5 Right UV curing module Allows you to monitor the specific properties of the right UV curing module 6 Z O...

Страница 37: ...u can choose to cancel the printing or resume 7 8 9 7 Pause Tap to pause the bioprinting process 8 Resume Tap to resume the bioprinting process This option is only available when the bioprinting proce...

Страница 38: ...ption to start bioprinting droplet without having to create a gcode for it BIO X will guide you through the process and help you to set up for a perfect bioprint Choose Print surface Select the wellpl...

Страница 39: ...o use the printhead or not If you select to use the printhead further settings will appear 3 Bioink profile Select the bioink profile you want to use or create a new one BIO X comes preloaded with pro...

Страница 40: ...he settings can be reviewed Should a setting or value be incorrect you can easily go back to that step and correct it by pressing the navigation bar at the bottom of the screen 1 1 Printhead 1 Setting...

Страница 41: ...oggle the ABL option on off The ABL should be enabled after calibrating the surface probe and going back to the Printhead 1 tab 6 Z axis command controls the position of the printbed along the z axis...

Страница 42: ...erview of how far the bioprinting process has come and how long it will take until it is completed 3 Bioprint Tap to start the bioprinting process Caution Do not open the door without pausing the biop...

Страница 43: ...e USB drive insert it in the Bio X You can then access it via this tab and procede to the updating of your Bio X 4 4 Utilities 2 1 1 USB content list allows you to select the desired update in the lis...

Страница 44: ...want to start Bioprinting You can also choose to pre cool the printbed 4 4 Utilities 2 4 3 1 Printhead 1 temperature Use this to set the temperature of the printhead 1 2 Printhead 2 temperature Use t...

Страница 45: ...aterial being used This pressure mainly depends on the viscosity of the material 4 4 Utilities 2 1 1 Set pressure for each printhead 0 200kPa when using the internal air compressor 2 Test bioink flow...

Страница 46: ...stomer support purposes 4 4 Utilities 1 2 3 1 Enable dark mode turn off all the chamber lights This allows you to work with photo sensitive material 2 Clean chamber fan Toggle the clean chamber fan op...

Страница 47: ...5 6 1 2 3 1 Printhead 1 test movement for printhead 1 2 Printhead 2 test movement for printhead 2 3 Printhead 3 test movement for printhead 3 4 Z axis command controls the position of the printbed alo...

Страница 48: ...us to check and test the different sensors 4 4 Utilities 1 2 3 2 3 1 Output data Allows customer support to check and test the different censors 2 License Gives you access to all the licenses of the...

Страница 49: ...Maintenance...

Страница 50: ...ves and sterilize them using 70 ethanol Remove the four screws holding the HEPA filter in place 2 Replace the HEPA filter with a clean one Fasten the clean HEPA filter with the four screws and plug in...

Страница 51: ...ug BIO X before cleaning it with ethanol Then simply spray the surface you wish to sterilize and wipe the surface with a soft cloth Caution If ethanol has been used to clean the printchamber of BIO X...

Страница 52: ......

Страница 53: ...Terms and Conditions...

Страница 54: ...o assume that a delay in the agreed delivery could occur Cellink shall notify the Buyer thereof and shall in such case be entitled to reasonable prolongation of the delivery date of up to twenty 20 bu...

Страница 55: ...any partial delivery will be regarded as filling a specific order 5 ACCEPTANCE The Buyer shall immediately following each delivery of any Product inspect the Product in order to identify any external...

Страница 56: ...writing within the above time limits the Buyer forfeits its right to make any claim in respect of the defect If there is reason to believe that the defect may cause damage notice shall be given forthw...

Страница 57: ...s only allowed to use the Product Software for your own benefit and you are not allowed to make the application available to third parties 9 TRADEMARKS AND OTHER INTELLECTUAL PROPERTY RIGHTS All intel...

Страница 58: ...event that a Product or parts thereof becomes subject to a recall decided by Cellink the Buyer shall participate and assist in such a process in accordance with Cellink s instructions The Buyer is en...

Страница 59: ...hs or more each Party shall be entitled to terminate the Agreement 14 CONFIDENTIALITY The Parties hereby undertake during the term of this Agreement and thereafter to hold in confidence and absolute s...

Страница 60: ...General Terms shall be interpreted and construed in accordance with the laws of Virginia The United Nations Convention on Contracts for the International Sale of Goods shall not apply Any dispute con...

Страница 61: ...INK holds all right title and interest in and to the CELLINK 3D Bioprinter Software Licensed Software and all associated websites platforms and applications and is the sole and exclusive owner of http...

Страница 62: ...e agrees to download and install any and all updates when available and acknowledges that if not updated as recommended the Licensed Software may not operate as expected Licensee acknowledges and agre...

Страница 63: ...icensee agrees that CELLINK is not responsible or liable in any way for any of Licensee s information or content CELLINK and its service providers may use access and retain Licensee s information or c...

Страница 64: ...AND CELLINK 3D BIOPRINTER IS AT LICENSEE S OWNRISK CELLINKANDITSSUPPLIERSHEREBYDISCLAIMALLWARRANTIES EXPRESS IMPLIED OR STATUTORY INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY...

Страница 65: ...CES FILES AND DATA AND DAMAGE TO LICENSEE S OR A THIRD PARTY S PROPERTY VIRUSES HARMFUL CODE OR OTHER HARMFUL COMPONENTS INCOMPATIBILITY WITH SOFTWARE SYSTEMS OR SERVICES INACCURACIES INCOMPLETENESS C...

Страница 66: ...TE RESULTING FROM PRINTING ANY DESIGN USING THE CELLINK 3D BIOPRINTER AND OR LICENSED SOFTWARE 12 INDEMNITY Licensee agrees to indemnify defend and hold harmless CELLINK its subsidiaries officers dire...

Страница 67: ...in a writing signed by CELLINK Any other modification to this User License must be made by written instrument duly executed by the Parties This User License and its formation operation and performance...

Страница 68: ...that a customer has already been through all these previous options stated and is still not satisfied with the results the customer must have the bioprinter sent back at their own expense Once a retu...

Страница 69: ...tial 3 months warranty starting on the purchase date Components not included in the warranty include timing belts linear ball bearings rods and the build platform Moving parts although of the highest...

Страница 70: ...ermined in calculation with the FedEx tracking information Limitations This guarantee and limited warranty are sole and final as they stand in reference to BIO X CELLINK and it s products where permit...

Страница 71: ......

Страница 72: ...Printhead manuals...

Страница 73: ...his to securely fasten the cartridge in the printhead Nozzle or needle Printhead contact The connector between BIO X and the intelligent printhead Magnet The magnet allows for securely fastening the p...

Страница 74: ...pply in order to function Do not forget to connect the pressure hose to the pressure connector on the printhead holder when inserting the printhead in BIO X 1 2 3 Attach the pressure hose to the cartr...

Страница 75: ...Imagination is the beginning of creation Youimaginewhatyoudesire you will what you imagine and at last you create what you will George Bernard Shaw Happy 3D bioprinting...

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