6 | Software
Büchi Labortechnik AG
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Operation Manual Sepiatec SFC-250
6.5.1
Setting peak control
When using a fractionation mode with peak detection the programmed number of
peaks are compared with the actual detected number of peaks at the end of each
injection. The run stops automatically if the peak detection is not successful.
The number of peaks to be collected is determined by touching the appropriate peak
number. The operator can specify the fractionation start and stop time and the
sample bottle in which the fractions are collected for the number selected. The start
time of the following peak must be higher than the stop time of the preceding peak.
The stop time of the final fraction must be lower than the total separation run time.
Tap the
[Peak No.]
button if no peaks are to be collected. This deactivates the input
fields.
The peak control feature allows the system to recognize automatically when a peak
is outside the collection area and either stop the run or correct the collection
window accordingly.
If a mode with peak detection is used, the number of detected peaks has to be the
same as the number of programmed peaks, otherwise the run will stop. This test is
performed even if the Peak Control is switched off.
Navigation path:
➔
COLLECTION
➔
[Peak control]
u
Tap the
[OFF]
button to deactivate peak
control.
u
Tap the
[ON]
button to activate peak control.
ð
A new window will open to set the control
conditions.
u
Set the control conditions.
u
Tap the
[OK]
button to confirm.
6.5.2
Setting stack injection
The stack injection in isocratic mode allows time saving fractionation of large
samples. The sample portions are injected after the elution of a peak.
Navigation path:
➔
COLLECTION
