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3 | Product description

Büchi Labortechnik AG

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Operation Manual Sepiatec SFC-250

3

Product description

3.1

Description of function

The instrument is for preparative separations on small columns by means of
supercritical fluid chromatography (SFC).

Supercritical fluid chromatography has the ability to separate gram size samples.

The integrated gas-liquid-separators (GLS) allow fast and efficient removal of CO

2

from separated components.

The instrument allows:

A mix of supercritical CO

2

and one solvent.

With the optional solvent selection valve, the solvent can be chosen from four
different solvents.

Injection of liquid or solid sample

Separation on a column

Detection of the components by using a UV, ELSD or MS detector.

Collecting the desired fractions

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Содержание Sepiatec SFC-250

Страница 1: ...Sepiatec SFC 250 Operation Manual 11594446 A en...

Страница 2: ...the right to make changes to the manual as deemed necessary in the light of experience especially with respect to structure illustrations and technical details This manual is copyrighted Information f...

Страница 3: ...7 6 Malfunction of a connected instrument option 9 2 7 7 Malware infection due to connections with other devices or network 10 2 8 Modifications 10 2 9 Warning signs 10 3 Product description 12 3 1 De...

Страница 4: ...6 6 2 Controlling the CO valve 40 6 6 3 Controlling the purge pump 41 6 7 RUN METHOD tab 42 6 7 1 Before starting a run 43 6 8 Changing during a run 44 6 8 1 Change No of Injections 44 6 8 2 Change i...

Страница 5: ...ion Manual Sepiatec SFC 250 v 9 Help with faults 59 9 1 Troubleshooting 59 10 Taking out of service and disposal 60 10 1 Taking out of service 60 10 2 Disposal 60 10 3 Returning the instrument 60 11 A...

Страница 6: ...haracter draws attention to a requirement that must be met before the instructions below are carried out u This character indicates an instruction that must be carried out by the user This character i...

Страница 7: ...at the following target groups Users Users are persons that meet the following criteria They have been instructed in the use of the instrument They are familiar with the contents of these operating in...

Страница 8: ...risk which could result in death or serious injury if not prevented WARNING Indicates a danger with a medium level of risk which could result in death or serious injury if not prevented CAUTION Indica...

Страница 9: ...t process any unknown fluids u Observe the safety data sheets for all substances used 2 7 4 Dangerous particles The use of the instrument can produce dangerous particles that can cause life threatenin...

Страница 10: ...accessories spare parts and consumables u Carry out technical changes only with prior written approval from BUCHI u Only allow changes to be made by BUCHI service technicians BUCHI accepts no liabilit...

Страница 11: ...ING Pressurized gas The operating instrument is pressurized Venting of pressurized gases and solvents is possible CO2 is suffocating in high concentrations u Install gas detectors at site u Create suf...

Страница 12: ...graphy has the ability to separate gram size samples The integrated gas liquid separators GLS allow fast and efficient removal of CO2 from separated components The instrument allows A mix of supercrit...

Страница 13: ...uid is a condensed gas above the critical temperature and pressure Its properties are those of a gas and liquid Carbon dioxide CO2 is mostly used The CO2 is transported by the pump in a cooled state T...

Страница 14: ...idual CO2 and optional in the organic from the add on pump The fractions are available in highly concentrated form for further analysis 3 2 Configuration 3 2 1 Front view 1 2 3 4 5 6 7 8 9 4 1 Touchsc...

Страница 15: ...escription 3 Operation Manual Sepiatec SFC 250 15 62 Column oven 1 2 3 4 5 6 7 7 8 1 Flow cell with optic fibre cable 2 Leak sensor 3 Injection valve 4 Column selector valve 5 Heating module 6 Filter...

Страница 16: ...peration Manual Sepiatec SFC 250 3 2 2 Electric and electronic connections 1 2 3 4 5 6 7 1 Fuses and FI circuit breaker 2 Main switch 3 USB connection 4 HDMI connection 5 LAN external connection 6 LAN...

Страница 17: ...for cooling water supply 2 Outlet CO2 safety relief valve 3 Connection for CO2 supply 3 2 4 Exhaust connections There are three exhaust connections on this instrument On the left side is the exhaust...

Страница 18: ...ion degree 2 Minimum clearance on all sides 200 mm Controller 15 6 touchscreen 16 9 format System Integrated Windows 10 Control software Prep SFC control software Operating temperature range 15 25 C O...

Страница 19: ...ions For indoor use only Max altitude above sea level 2 000 m Ambient and storage temperature 15 25 C Maximum relative humidity 45 75 not condensing 3 4 3 Solvents CO2 Liquid Organic modifier solvents...

Страница 20: ...duct description B chi Labortechnik AG 20 62 Operation Manual Sepiatec SFC 250 Flow rate accuracy 2 at 2 50 of the flow range with methanol water 80 20 v v Precision 0 25 from 1 ml min to 20 ml min at...

Страница 21: ...the carrier u Keep packaging for future transportation 4 2 Storage u Make sure that the ambient conditions are complied with see Chapter 3 4 Technical data page 18 u Wherever possible store the device...

Страница 22: ...k the shipment unless expressly agreed NOTE Make sure that the power supply can be disconnected at any time in an emergency A BUCHI service engineer or an authorized representative will unpack and che...

Страница 23: ...as waste must be at least 10 mm The instrument must be at least 200 mm away from adjacent walls or other instruments The distance at the reverse side should be at least 100 mm This ensures adequate ve...

Страница 24: ...ause of not suitable power supply cables Not suitable power supply cables can cause bad performance or an instrument damage u Use only BUCHI power supply cables Precondition R The electrical installat...

Страница 25: ...data To confirm the data tap the OK button The dialog box closed automatically and the data is visible in the display field All display fields are shown in this operation manual Depending on the conf...

Страница 26: ...er names u Tap on an entry field The display shows a dialog with an alphanumeric input box u Enter the value u Tap the OK button to confirm The value is saved The dialog box closes 6 1 3 Exiting the s...

Страница 27: ...n the method which has been loaded and can be adjusted individually for each run See SYSTEM tab 6 RUN METHOD tab To start a loaded method and view online This window is used to start the current run w...

Страница 28: ...sure is too high the system cannot be started CO2 VALVE yellow orange blink The CO2 inlet pressure is too low The pumps can be manual started for a flush Starting an automated run is not possible 8 LA...

Страница 29: ...u Enter the value u Tap the OK button to confirm The value is saved The dialog box closes u Tap the END INJECTION button to stop the injection u Tap the STOP PUMP to stop the pump NOTICE An automatic...

Страница 30: ...isplayed u View parameter table u Tap the button to activate or deactivate u Tap the BACK TO MAIN VIEW button to close the service view window The window closes automatically 6 4 PARAMETER tab The par...

Страница 31: ...be carried out one af ter the other 13 Modifier Selection of pump inlet at the modifier pump 6 4 1 Setting new methods Navigation path PARAMETER u Tap the METHOD NAME button The display shows a dialo...

Страница 32: ...e modifier and flow values u Enter the value u Tap the OK button to confirm The value is saved The dialog box closes NOTICE The necessary volume of CO is calcu lated automatically u Tap the ADD button...

Страница 33: ...parameter in the methods NOTICE The number of injections to be carried out in sequence is not saved in the method and must be specified again whenever reloading the method or starting the system 6 4...

Страница 34: ...ak based fractionation 6 T P button Switches on the combined fractionation In this mode the peaks are searched inside the programmed intervals 7 Peak No Number of peaks to be collected 8 Vial Fraction...

Страница 35: ...which chro matogram line is shown in the overview chart on the RUN menu V Intermediate part between the peaks the Valley NOTE Up to eight fractions in total can be collected All parts of a run that a...

Страница 36: ...er than max shift 4 Max Shift The maximum shift difference is in seconds The maximum allowed shift from the reten tion time observed during the first injection 5 Min Height The minimum height is in pe...

Страница 37: ...Name Description 1 First injection Chromatogram of the first injection 2 Multiple injections Chromatogram of multiple injections 3 Stack injections Chromatogram of stack injection 4 Stack time The tim...

Страница 38: ...n time Tap the Peak No button if no peaks are to be collected This deactivates the input fields The peak control feature allows the system to recognize automatically when a peak is outside the collect...

Страница 39: ...er than the runtime but long enough to include all fractionations 6 6 SYSTEM tab 1 2 3 4 5 6 7 8 9 11 10 No Name Description 1 SYSTEM tab To configurate system settings The system parameters are set i...

Страница 40: ...right hand corner The white field shows the status of the back pressure regulator Navigation path SYSTEM Precondition R Instrument is running Displayed BPR status BPR is active u Tap the RELEASE butto...

Страница 41: ...Tap the Purge Modifier Pump or Purge Add on Pump button A new window opens to set modifier pump u Tap the Open button to release the purge valve from the pumps NOTICE The Purge Add on Pump has just o...

Страница 42: ...f multiple injections In case of stack injection the collected frac tions are displayed overlaid 4 Parameter status The status of individual system parameters and method parameters during the run is s...

Страница 43: ...ACK PRESSURE button orange The back pressure regulator valve is active The pumps can be started A run can also been started but the equilibration time only starts counting after the back pressure has...

Страница 44: ...reased volume the next injection will wait the necessary amount of time This will not change the run time NOTE The fractionation parameters can be adjusted starting with the second fractionation NOTE...

Страница 45: ...CANCEL button to close the window without any changes u Tap the OK button to update the version of the solvent amount The window closes automatically 6 8 2 Change injection and run time parameter Navi...

Страница 46: ...e the version of the solvent amount The window closes automatically 6 8 3 Change fraction parameter Navigation path RUN METHOD Change NOTICE This window close automatically after 10 seconds if no sele...

Страница 47: ...n to change the value 0 01 u Tap the button to change the value 0 05 u Tap the button to change the value 0 05 u Tap the OK button to make the changes effective 6 9 CO recycling module Navigation path...

Страница 48: ...o the manual position 12 Inject Valve to Load button To switch the injection valve into the load po sition 13 Expansion Valve to Inject button To switch the expansion valve into the injec tion mode 14...

Страница 49: ...indicates how often the sensor has detected liquid This value is an indication how stable the recycling and the gas liquid separators are working A low value means stable conditions A high value mean...

Страница 50: ...separators GLS are also under pressure The fractionated liquid is fed to the recycling module via pressure stable flexible lines A valve storage tube valve combination is available for each of the ten...

Страница 51: ...on the system with the main switch This also starts the computer and the touchscreen u Release the emergency switch button u Press the green button next to the emergency switch u Tap the Prep SFC sym...

Страница 52: ...ctionation valve u Place the fraction bottles in the positions specified in the collection menu u Attach the appropriate capillary tubes of the gas liquid separators to the fraction bottle u Make sure...

Страница 53: ...y stopped Navigation path PARAMETER Precondition R Make sure there is enough CO2 and solvents to complete the separation run of the sample The eluent consumption is highly dependent on the used method...

Страница 54: ...to stopped u Tap the Immediately button The run stops immediately 7 7 Shutting down the system Navigation path MANUAL u Tap the EXIT button to close the Prep SFC control software u Shut down the wind...

Страница 55: ...rts required are contained in the Rhebuild kit 8 3 Cleaning the UV detector flow cell Precondition R The detector baseline starts to get noisy Possible sample residues are in the flow cell u Remove th...

Страница 56: ...u Check the rotor surface for scratches Replace the rotor if there is any damage u Check the surface of the stator u Replace it if there are any scratches between the connections Contact BUCHI Custom...

Страница 57: ...BUCHI Customer Service if problems persist 8 6 Gas liquid separators GLS NOTE To avoid contamination in the collected fractions it is recommended to flush the gas liquid separators with a CO modifier...

Страница 58: ...technik AG 58 62 Operation Manual Sepiatec SFC 250 u Carefully pull the blue insulation vertically downwards from the GLS u Use the GLS key provided to open the GLS by turning it to the left u Hold th...

Страница 59: ...use or FI circuit breaker Computer or touchscreen defective u Contact BUCHI Customer Service Unusually high pres sure Pump defective u Contact BUCHI Customer Service Blockages in the capillary system...

Страница 60: ...tubing and communication cables from the device 10 2 Disposal The operator is responsible for proper disposal of the instrument u When disposing the equipment observe the local regulations and statuto...

Страница 61: ...1 Appendix 11 1 Spare parts and accessories Use only genuine BUCHI consumables and spare parts in order to ensure correct safe and reliable operation of the system NOTE Any modifications of spare part...

Страница 62: ...We are represented by more than 100 distribution partners worldwide Find your local representative at www buchi com...

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