Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 8 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
8.12.29.
Wait for the pH value to reach its setpoint before proceeding.
8.13.
Activate additional feed loops at the appropriate time as indicated by the process
SOP.
8.13.1.
Activate a feed loop by selecting the box in the “Loop Name” row of the “pH 1”
column. This box should be displaying the column heading “pH 1”. Press the
ALTER key until the “Feed 2” column heading appears (or the appropriate feed
column heading if not “Feed 2”).
8.13.2.
Enter the appropriate setpoint for the feed solution, as indicated by the process
SOP, by choosing the box in the “Set” row of the “Feed 2” column. Use the
number keypad to enter the appropriate setpoint value and then press the ENTER
key.
8.13.3.
In the “Control” row of the “Feed 2” column press the ALTER key until “on” is
displayed, then press ENTER.
8.14.
Calibrate the Dissolved Oxygen Probe
Note: The DO probe should not be calibrated until the desired working temperature has been
reached.
8.14.1.
Polarize the DO probe for 30 minutes prior to performing calibration.
8.14.1.1.
If necessary, connect the DO cable to the DO electrical outlet on the front of
the cabinet.
8.14.1.2.
Remove the protective cap from the top of the DO probe and connect the
DO Cable to the DO probe.
8.14.1.3.
If necessary, turn on the BioFlo 3000.
8.14.1.4.
Allow the probe to sit for at least 30 minutes.
8.14.1.5.
Verify that the DO level is above 0. If the DO display reads 0 or below there
may be a problem with the DO probe. Contact the lab manager if this
occurs.
8.14.2.
Use the SCREEN key to choose the <Calibration> screen and then press the
ENTER key.
8.14.3.
Use the arrow keys to choose the box in the “DO” row of the “Function” column.
This box should be displaying “Read”.
8.14.4.
Change “Read” to “Zero” by pressing the ALTER key until “Zero” appears and
then pressing the ENTER key.
8.14.5.
Use the arrow keys to choose the box in the “DO” row of the “Zero” column.
Enter “0.0” using the number keypad and then press the ENTER key.
8.14.6.
Use the SCREEN key to choose the <Master> screen and then press the ENTER
key.
8.14.7.
Use the arrow keys to choose the box in the “Set” row of the “Agit 1” column.
8.14.8.
Enter “1000” (for 1000rpm) using the number keypad and then press the ENTER
key. This will turn on the agitation motor.
8.14.9.
Press the SCREEN key and then use the arrow keys to select the <Gases> screen
and then press the ENTER key.
8.14.10.
Set the gases mode to “Manual” by selecting the box in the “Mode” row (it is the
only box available in the “Mode” row). Press the ALTER key until “Manual” is
displayed, then press the ENTER key.
18
