Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 7 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
8.12.20.
Set the control mode to P-I-D by selecting the box in the “Control” row of the
“Temp 1” column. Press ALTER until “P-I-D” appears and then press the
ENTER key.
8.12.21.
Set the agitation loop to P-I-D control by selecting the box in the “Control” row
of the “Agit 1” column. Press the ALTER key until “P-I-D” appears and then
press the ENTER key.
8.12.22.
Set the agitation setpoint to 100rpm by selecting the box in the “Set” row of the
“Agit 1” column. Use the number keypad to enter 100 and then press the ENTER
key.
8.12.23.
Set up the feed solution flasks on the appropriate feed pumps, as indicated by the
process SOP. Acidic or basic solutions should be set up on Feed 1.
Refer to the
process SOP for indication of an acidic or basic solution.
8.12.23.1.
Unclamp the tube attached to an addition port and remove the glass wool
and aluminum foil.
8.12.23.2.
Thread the free end of the tube through the bottom of the feed pump,
counter clockwise around the pump, and out the top.
8.12.23.3.
Place the free end of the tube on the top of the glass pipet of the feed
solution flask.
Note: The pump moves in a clockwise direction. Therefore the solution
will flow from right to left in the tube.
8.12.24.
Repeat step 8.12.23 for additional feed solution flasks, if needed.
8.12.25.
Set the pH setpoint to the desired value. Refer to the process SOP for the desired
pH.
8.12.25.1.
Set the pH setpoint by selecting the box in the “Set” row of the “pH 1”
column. Use the number key pad to enter the desired pH and then press the
ENTER key. Do this step even if the desired pH was already displayed
when selecting the box.
8.12.26.
Set the pH loop to P-I-D control by selecting the box in the “Control” row of the
“pH 1” column. Press the ALTER key until “P-I-D” appears and then press the
ENTER key.
8.12.27.
Designate Feed 1 as acid or base. Refer to the process SOP to determine if the
Feed 1 solution is acidic or basic.
8.12.27.1.
Designate Feed 1 by using the arrow keys to select the box in the “Loop
Name” row of the “pH 1” column. This box should be displaying the
column heading “pH 1”. Press the ALTER key until the “Feed 1” column
heading appears then press the ENTER key.
8.12.27.2.
Use the arrow keys to select the box in the “Control” row of the “Feed 1”
column. Use the ALTER key to select either “Base” or “Acid” and then
press the ENTER key.
8.12.27.3.
Set the setpoint to 100 by choosing the box in the “Set” row of the “Feed
1” column. Use the number keypad to enter “100” and then press the
ENTER key.
8.12.28.
Return to the pH loop display by selecting the “Feed 1” column heading. Press
the ALTER key until “pH 1” appears and then press the ENTER key.
17
