Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 12 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
8.17.4.
From the desktop open BioCommand Lite by double clicking on the BCLite
icon.
8.17.5.
You can choose to use an existing recipe or create a new recipe. To use an
existing recipe follow the procedure in step 8.17.6. To create a new recipe follow
the procedure in step 8.17.7.
8.17.6.
To use an
existing recipe
, choose “Open an Existing Recipe” and then click
“Continue”.
8.17.6.1.
Select the appropriate recipe (i.e., Pichia Master 1.rcp).
8.17.6.2.
Verify that data is collected at 60min intervals in the “Time Data” tab under
“Log Interval (Mins)”. If the Log Interval is not 60, change it to 60 using the
following procedure.
8.17.6.2.1.
Click on “Change Interval”
8.17.6.2.2.
Select “60” from the pull down menu.
8.17.6.2.3.
Click “Save”.
8.17.6.3.
Click on the “Views” tab, and double click “Trend.3” in the column on the
left under “Current Views”.
8.17.6.4.
Verify that the Trend.3 ranges are appropriate per the process SOP.
8.17.6.5.
On the main screen, click “Start Batch”.
8.17.6.6.
Save batch file as the product lot number (i.e. 21Feb08HSA).
8.17.6.7.
Skip step 8.17.7. and continue with the SOP at step 8.17.8.
8.17.7.
To create a
new recipe
, from the “BioCommand Lite” window select “Create a
new recipe with Batch Wizard” and click “Continue”.
8.17.7.1.
In the “BioCommand Batch Wizard-Introduction” window click “Next”.
8.17.7.2.
In the “BioCommand Batch Wizard –Step 1” window type in “Recipe1”
under “Enter Recipe Display Name:” and click “Next”.
8.17.7.3.
In the “BioCommand Batch Wizard-Step 2” window click “Select Loops”.
8.17.7.4.
Click on “Browse Local Server”.
8.17.7.5.
Under “Available Loops:” highlight “BIOLAB01” then click “>>”. This
adds “BIOLAB01” to the “Loops in Batch” box.
8.17.7.6.
Under “Available Loops:” highlight “Controller 1” and click “Browse
Local Server.”
8.17.7.7.
After the + sign appears next to “Controller 1” click “>>”.
8.17.7.8.
Click “Close”.
8.17.7.9.
When the “Biocommand Batch Wizard –Step 2” window reappears, click
“Next”.
8.17.7.10.
The “Biocommand Batch Wizard –Step 3” window should appear with the
recipe file name. File name format should be:
“C:\BIOCOM~1\BIORcp[#].rcp”. Click “Next”.
8.17.7.11.
In the “Biocommand Batch Wizard –Step 4” window click “Next”.
8.17.7.12.
In the “Biocommand Batch Wizard – Finished!” window, click “Finish”.
8.17.7.13.
In the “Recipe[#].B1:Setup.[#]” window, select “Start Batch”.
8.17.7.14.
If needed, type in “Recipe1” in the “Batch Display Name” window.
(“Recipe1” may automatically appear.). Click “OK”.
8.17.7.15.
In the “Biocommand Save Files” window save batch file as:
22
