Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 15 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
the sensory tips of these probes. Return each probe to the probe stand after
cleaning.
8.22.17.
Add pH probe storage solution (3M potassium chloride) to the protective cover
of the pH probe. The cover should be approximately half full of solution.
8.22.18.
Carefully cover the pH probe with the protective cap. The probe should be
immersed in pH probe storage solution.
8.22.19.
Place the DO probe protective cap on the bottom of the DO probe.
8.22.20.
DO and pH probes should be stored in an upright position in the probe stand.
8.22.21.
Clean the headplate and impellers with water and glassware detergent. A soft
brush or sponge can be used. Rinse thoroughly with tap water and then
deionized water.
8.22.22.
Remove the O-ring from the headplate and heat exchanger base and clean
thoroughly.
8.22.23.
Clean the heat-exchanger base and rinse thoroughly.
8.22.24.
Ensure that all vessel parts have been cleaned and rinsed thoroughly. Allow
them to air dry.
8.22.25.
Wipe down the BioFlo cabinet with 10% bleach solution.
9.
Attachments:
9.1.
Figure 1: BioFlo 3000 Vessel and Cabinet
9.2.
Figure 2: Vessel Parts
9.3.
Figure 3: Headplate, Underside
9.4.
Figure 4: Assembled Headplate, Top Side
9.5.
Figure 5: BioFlo 3000 Side View
9.6.
Figure 6: BioFlo 3000 Controller
10.
History:
Name Date
Amendment
Sonia Wallman
1997
Initial release
Sue Penney
2003
Updated.
Karl J.
Cresswell
121905
Removed all grease steps and added inspection of DO probe.
Deb Audino
20July07 Added Feed 1 and 2 low and high values. Added inputing DO
setpoint.
Deb Audino
04Apr08 College name change. Added steps for using an existing recipe.
Kari Britt
15May09 Changed directions for calibration of the pH meter and dissolved
oxygen probes. Changed directions in “Prepare for Operation”
section. Changed directions for assembling the headplate.
Changed directions for the three types of control modes. Clarified
directions for using BCLite. Removed figures and added
additional figures in the attachments section.
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