Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 5 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
8.8.12.
Immerse the pH and RTD temperature probes into a pH 4 standard buffer
solution. Allow a few minutes for the electrode to equilibrate.
8.8.13.
Use the arrow keys to choose the box located in the “pH” row of the “Span”
column. Enter “4.0” using the number keypad and then press the ENTER key. Do
this step even if “4.0” was already displayed when selecting the box.
8.8.14.
Rinse the pH and RTD temperature probes with deionized water and pat dry with
a laboratory tissue.
8.8.15.
Disconnect the pH cable from the pH probe, and replace the shorting cap.
8.8.16.
Inspect the integrity of the internal O-ring of pH probe port. Replace if worn or
cracked.
8.8.17.
Apply a small amount of deionized water to the pH probe and then insert it into
the pH probe port.
8.8.18.
Ensure that the pH probe electrode is not touching the baffle.
8.9.
Install Dissolved Oxygen Probe
8.9.1.
Remove the protective cap
from the bottom of the DO probe and inspect the
screen. Replace the screen if damaged.
8.9.2.
Remove the bottom housing
that encases the DO probe tip.
8.9.3.
Inspect the integrity of the O-ring and replace if worn or cracked.
8.9.4.
Replenish the DO electrolyte in the bottom housing.
8.9.5.
Replace the bottom housing.
8.9.6.
Carefully insert the DO probe into the DO port of the headplate. Tighten the
adaptor with a wrench.
8.9.7.
Ensure that the DO probe is not touching the baffle.
8.10.
Attach Tubing
8.10.1.
Attach silicone tubing to the harvest port, bend the tube in half and secure with a
clamp. Wrap glass wool and aluminum foil around the exposed end.
8.10.2.
Place a piece of PharmMed tubing (approximately 1.5 meters in length) onto each
addition port, bend the tubes in half and secure with clamps. Wrap glass wool and
aluminum foil around the exposed ends.
8.10.3.
Place silicone tubing on the top outlet of the condenser and connect an air filter.
Wrap glass wool and aluminum foil around the exposed end of the filter.
8.10.4.
Place silicone tubing on the top of the sparger tube and connect an air filter.
Bend the tube in half and secure with a clamp. Wrap glass wool and aluminum
foil around the exposed end of the tubing.
8.10.5.
Remove the rubber bulb from the sampler assembly. Insert glass wool into the
bulb and reattach it to the sampler assembly.
8.11.
Autoclave the vessel
8.11.1.
Turn off the BioFlo 3000.
8.11.2.
Turn off the water valve (under the lab bench).
8.11.3.
Disconnect the water lines to the heat exchanger.
8.11.4.
Disconnect the water lines to the condenser.
8.11.5.
Double check all tubes and ports to ensure that the vessel is completely sealed.
8.11.6.
Autoclave the entire vessel and heat exchanger assembly for at least 30 minutes
per autoclave SOP.
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