Great Bay Community College
Document
Number:
1.16.1
320
Corporate
Drive
Revision
Number:
5
Portsmouth,
NH
03801 Effective
Date:
20May09
Page 6 of 20
Title: New Brunswick BioFlo 3000 Bioreactor SOP
8.12.
Prepare for Operation
8.12.1.
Remove the clamp from the tubing attached to the top outlet of the condenser.
8.12.2.
Place the vessel and heat exchanger base on the platform in front
of the cabinet so that it fits on the three steel plugs. The heat exchanger
connectors should face the front left corner of the platform. See Figure 1 for
proper orientation of the heat exchanger base.
8.12.3.
It is advantageous to begin polarization of the DO probe at this point in the
procedure. Follow directions in step 8.14.1 to do this, and then return to step
8.12.4. to connect the water lines.
8.12.4.
Connect the “Jacket Water Out” water line to the top heat exchanger connector
using the quick connects adaptors.
8.12.5.
Connect the “Jacket Water In” water line to the bottom heat exchanger connector
using the quick connects adaptors.
8.12.6.
Connect the “Condenser Water Out” water line to the top condenser connector
using the quick connects adaptors.
8.12.7.
Connect the “Condenser Water In” water line to the bottom condenser connector
using the quick connects adaptors.
8.12.8.
Turn on the water. The water valve is located under the lab bench. The pressure
gauge should read between 15 and 20psi.
8.12.9.
Add approximately 1mL of glycerol to the thermowell and insert the RTD
temperature probe into the thermowell.
8.12.10.
Remove the shorting cap from the top of the pH probe and connect the pH cable
to the pH probe.
8.12.11.
Connect the air line from the sparger tube to the sparger gas port located on the
front of the cabinet, near the top.
8.12.12.
Turn on the air supply.
8.12.13.
The air pressure gauge should not exceed 10psi (5psi is the ideal pressure level).
8.12.14.
Remove the agitator cover from the top of the headplate and place the agitator
motor
securely in the agitator shaft.
8.12.15.
Turn on the BioFlo 3000.
8.12.16.
Select Fermentation mode (2) and press the ENTER key.
8.12.17.
If the Master screen does not automatically appear use the SCREEN key to select
the <Master> screen and then press the ENTER key.
8.12.18.
Set the temperature control mode to “Prime” by using the arrow keys to select
the box in the “Control” row of the “Temp 1” column. Press the ALTER key
until “Prime” is displayed and then press the ENTER key.
8.12.19.
Wait 5 minutes before setting the working temperature. Refer to the process
SOP for the desired working temperature.
8.12.19.1.
Set the working temperature by using the arrow keys to select the box in
the “Set” row of the “Temp 1” column. Use the number keypad to enter
the desired temperature and then press the ENTER key. Do this step even
if the desired temperature was already displayed when selecting the box.
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