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Brunswick BIOFLO 3000, Руководство пользователя

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Brunswick BIOFLO 3000 Скачать руководство пользователя страница 18

Great Bay Community College 

    Document 

Number: 

1.16.1 

320 

Corporate 

Drive 

      Revision 

Number: 

5 

Portsmouth, 

NH 

03801      Effective 

Date: 

20May09 

 

 

 

 

 

 

        Page 18 of 20                                 

Title: New Brunswick BioFlo 3000 Bioreactor SOP

 

 

 

 

Figure 3: Headplate, Underside 

 

 
 
 

1.

 

Addition Port 

2.

 

Thermowell Port 

3.

 

O-ring 

4.

 

Foam Sensor Port 

5.

 

pH Probe Port 

6.

 

Agitator Shaft 

7.

 

Upper Impeller 

8.

 

Lower Impeller 

9.

 

DO Probe Port 

10.

 

Sampler Assembly Port 

11.

 

Inoculation Port 

12.

 

Harvest Tube Port 

13.

 

Sparger Tube Port 

14.

 

Addition Port 

 
 

28

Содержание BIOFLO 3000

Страница 1: ...llow the SOP as described and to inform the instructor about any deviations or problems that may occur while performing the procedure 4 References 4 1 New Brunswick BioFlo 3000 Bioreactor instrumentat...

Страница 2: ...the DO pH and temperature cables to their appropriate outlets on the front side of the cabinet of the BioFlo 3000 8 2 2 If necessary insert the plastic plugs into the holes in the glass vessel 8 2 2 1...

Страница 3: ...sion with the harvest tube 8 3 8 Secure the harvest tube in place with a nut by lowering the nut over the top of the harvest tube and hand tightening it to the threads on the topside of the headplate...

Страница 4: ...the water The water valve is located under the lab bench where the BioFlo 3000 is located The pressure gauge should read between 15 and 20psi when the water is turned on 8 8 Calibrate the pH Probe 8 8...

Страница 5: ...O electrolyte in the bottom housing 8 9 5 Replace the bottom housing 8 9 6 Carefully insert the DO probe into the DO port of the headplate Tighten the adaptor with a wrench 8 9 7 Ensure that the DO pr...

Страница 6: ...the lab bench The pressure gauge should read between 15 and 20psi 8 12 9 Add approximately 1mL of glycerol to the thermowell and insert the RTD temperature probe into the thermowell 8 12 10 Remove the...

Страница 7: ...rection Therefore the solution will flow from right to left in the tube 8 12 24 Repeat step 8 12 23 for additional feed solution flasks if needed 8 12 25 Set the pH setpoint to the desired value Refer...

Страница 8: ...tlet on the front of the cabinet 8 14 1 2 Remove the protective cap from the top of the DO probe and connect the DO Cable to the DO probe 8 14 1 3 If necessary turn on the BioFlo 3000 8 14 1 4 Allow t...

Страница 9: ...e Agit 1 column Press the ALTER key until P I D appears and then press the ENTER key 8 15 1 4 To set the minimum rpm select the box in the Set row of the Agit 1 column Use the number keypad to enter t...

Страница 10: ...set the minimum rpm select the box in the Set row of the Agit 1 column Use the number keypad to enter the desired minimum rpm as indicated by the process SOP and then press the ENTER key 8 15 3 5 Set...

Страница 11: ...then press the ENTER key 8 16 8 Use the arrow keys to select the box in the Control row of the Feed 2 column 8 16 9 Set the control mode to Level 1 by pressing the ALTER key until Lvl 1 appears and t...

Страница 12: ...e 21Feb08HSA 8 17 6 7 Skip step 8 17 7 and continue with the SOP at step 8 17 8 8 17 7 To create a new recipe from the BioCommand Lite window select Create a new recipe with Batch Wizard and click Co...

Страница 13: ...ck OK 8 17 7 19 8 Click 8 17 7 20 Repeat step 8 17 26 until all parameters needed are added to the Recipe B1 Trend window AGIT1 DO2 1 TEMP 1 and pH 1 should always be added 8 17 7 21 Set the graph ran...

Страница 14: ...ean with a soft brush if needed Rinse thoroughly with tap water and then with deionized water 8 22 8 Remove the vessel from the heat exchanger base and pour the bleach solution down the drain Rinse th...

Страница 15: ...oughly 8 22 23 Clean the heat exchanger base and rinse thoroughly 8 22 24 Ensure that all vessel parts have been cleaned and rinsed thoroughly Allow them to air dry 8 22 25 Wipe down the BioFlo cabine...

Страница 16: ...nger Connectors 4 Bottom Steel Ring 5 Glass Vessel 6 Condenser Water Lines 7 Top Steel Ring 8 Agitator Shaft 9 Agitator Cover 10 Condenser Top Outlet 11 Electrical Outlet 12 Sparger Gas Port 13 Agitat...

Страница 17: ...09 Page 17 of 20 Title New Brunswick BioFlo 3000 Bioreactor SOP Figure 2 Vessel Parts 1 Small Thumb Screw 2 Large Thumb Screw 3 Condenser 4 Agitator Cover 5 Agitator 6 Headplate 7 Sampler Assembly Bul...

Страница 18: ...18 of 20 Title New Brunswick BioFlo 3000 Bioreactor SOP Figure 3 Headplate Underside 1 Addition Port 2 Thermowell Port 3 O ring 4 Foam Sensor Port 5 pH Probe Port 6 Agitator Shaft 7 Upper Impeller 8 L...

Страница 19: ...k BioFlo 3000 Bioreactor SOP Figure 4 Assembled Headplate Top Side 1 DO Probe 2 pH Probe 3 Foam Sensor behind pH Probe 4 Condenser 5 Condenser Barbed Connectors 6 Ground Stud 7 Thermowell 8 Addition P...

Страница 20: ...ve Date 20May09 Page 20 of 20 Title New Brunswick BioFlo 3000 Bioreactor SOP Figure 5 BioFlo 3000 Side View 1 Gas and Air Console 2 Cabinet 3 Power Switch 4 Feed Pump Figure 6 BioFlo 3000 Controller 1...

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