ONCORE PRO, Operating Manual, Rev. K
26
4.5.
Loading Reagents
Oncore Pro detection kits, ancillary reagents and antibody/probe products are shipped ready-to-use.
Refer to the manufacturer’s Datasheet and SDS for specific instructions
.
Prepare all other reagent solutions following the manufacturer’s instructions.
Note: Only Oncore Pro reagent vials are compatible with the Oncore Pro Slide Stainer system.
Refer to the vialing
specifications in
Reagent Vialing Specifications
p.27, for additional information on vialing and recommended
reagent volumes.
Load the reagent vials onto the reagent rack and
remove all caps
.
Multiple vials of the same reagent may be placed on the rack. The system will prioritize the use of vials with the
earliest expiration date or the least number of tests remaining
. Refer to
Reagent Consumption Priority
, p. 69.
A.
For on-rack mixing of reagents, place an empty 7mL vial in the designated position #40 and/or #39 as
prompted by the system.
B.
For on-board mixing of reagents, avoid placing the second reagent component, Reagent B, in the last two
rows of the reagent rack as the Z2 probe will be unable to reach it.
Load the reagent rack onto the designated reagent plate on the instrument.
Verify that the rack is fully seated on the plate, and is oriented correctly, as shown in the figure below.
Figure 26
Note: On-board mixing reagents, such as Fast Red
are specially formulated to be mixed directly on the
slide:
The first mixing component, i.e.: Fast Red Substrate,
is applied with the Z1 probe. The second mixing
component, i.e.: Fast Red Chromogen, is applied
immediately afterwards with the Z2 probe.
The Z2 probe is specially designated for dispensing
the second mixing component; therefore, those
reagents, such as Red Chromogen, can only be
loaded in reagent rack positions #1-30.
