Beckman Coulter JA-14.50, Инструкция по эксплуатации

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PN B32164AC
20
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Run Speeds
The centrifugal force at a given radius in a rotor is a function of run speed. Comparisons of forces
between different rotors are made by comparing the rotors’ relative centrifugal fields (RCF). When
rotational speed is selected so that identical samples are subjected to the same RCF in two different
rotors, the samples are subjected to the same force (see
Table 5 ).
Do not select rotational speeds higher than the maximums you have determined to be appropriate
for your protocols (in no case above 14,000 RPM)
. When solutions more dense than 1.2 g/mL are
centrifuged in this rotor, use EQ 7 to calculate the reduced maximum allowable rotor speed.
EQ 7
Table 5
Relative Centrifugal Fields for the JA-14.50 Rotor
a
a. Entries in this table are calculated from the formula RCF = 1.12
r
(RPM/1000)
2
and then rounded to three significant digits.
Rotor Speed (RPM) Relative Centrifugal Field (

g )
At r
max
(160 mm) At r
av
(124 mm) At r
min
(87 mm)
14,000
13,000
12,000
11,000 35,000
30,300
25,800
21,700 27,200
23,500
20,000
16,800 19,000
16,500
14,000
11,800
10,000
9000
8000
17,900
14,500
11,500 13,900
11,200
8900
9700
7900
6200
7000
6000
5000 8800
6500
4500 6800
5000
3500 4800
3500
2400
4000
3000
2000
0
2900
1600
700
0
2200
1300
600
0
1600
900
400
0
reduced maximum speed = (14,000 RPM)
1.2 g/mL
density of tube contents
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