Beckman Coulter JA-14.50, Инструкция по эксплуатации

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PN B32164AC
4
JA-14.50 Fixed-Angle Rotor
Preparation and Use
Preparation and Use
Specific information about the JA-14.50 rotor is given here. Use the Rotors and Tubes for
Beckman Coulter J Series Centrifuges manual (publication JR-IM-10) and the centrifuge instruction
manual along with this manual for complete rotor and accessory operation information.
NOTE
Although rotor components and accessories made by other manufacturers may fit in the JA-14.50
rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’
components or accessories in the JA-14.50 rotor, excluding the tubes referenced in
Table 1 and Table 3 ,
may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the
components and accessories listed in this publication should be used in this rotor.
Prerun Safety Checks
Read the Safety Notice section at the front of this manual before using the rotor.
1
Make sure that the rotor, lid, and all tubes or accessories are clean and show no signs of
corrosion or cracking.
2
Verify that the tubes being used are listed in Table 1 , Table 2 , Table 3 and Table 4 .
3
Refer to Chemical Resistances (publication IN-175), included in the Rotors and Tubes CD-ROM for
the chemical compatibilities of all materials used.
Rotor Preparation
For runs at other than room temperature refrigerate or warm the rotor beforehand for fast equilibration.
1
Be sure that metal threads in the rotor are clean and lightly but evenly lubricated with Spinkote
lubricant (306812).
Also ensure that O-rings are lightly but evenly coated with silicone vacuum grease (335148).
2
Load the filled containers symmetrically into the rotor.
•
(Refer to Tubes for information about containers.)
•
If fewer than sixteen tubes are being run they must be arranged symmetrically in the rotor
(see
Figure 3 ).
•
Opposing tubes must be filled to the same level with liquid of the same density.