Beckman Coulter Avanti JXN-26, Руководство пользователя

Страница: 177 / 210

PN B38322AA B-1
APPENDIX B
Temperature Calibration Procedure
Introduction
The Avanti JXN series centrifuge specification for temperature control is
±
2°C. The following
procedure is provided for those cases in which temperature control within
±
1°C is required.
In this procedure, you will measure the temperature of your sample after equilibrating the rotor
temperature for a minimum of 1 hour. Based on the test results, you can then adjust the centrifuge
temperature setting to bring your sample as close as possible to the required temperature.
Calibrating Temperature
1
For runs at other than ambient temperature, precool or prewarm the rotor and test samples
to the required temperature.
The length of precooling/prewarming time depends on how different the rotor starting
temperature is from the required run temperature.
2
Install the rotor with adapters, if applicable, and tubes or bottles filled with sample buffer or
water (if above 2°C).
3
Set the speed to 2000 RPM, the time to
HOLD
, and the temperature to the required run
temperature.
a.
Start the run.
4
After 30 minutes, set the required run speed, and allow the system to run for at least 1 hour.
NOTE
The system must run for at least 1 hour before the temperature of the buffer or water is
measured . The length of time required for equilibration depends on the rotor and centrifuge starting
temperatures and the ambient room temperature.
5
After at least 1 hour, measure the temperature of the buffer or water using a thermometer or
thermocouple.