Chapter 7
Verifying the Instrument Performance
Troubleshooting
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
99
Notes
7
Troubleshooting
Troubleshooting – RNase P Plate Run
Condition: More than six outliers present in RNase P plate
data
Contact your Applied Biosystems service and sales
representative to order a replacement TaqMan RNase P
Instrument Verification Plate. If the replacement RNase P plate
fails, contact Applied Biosystems technical support or your
service representative for further assistance.
Condition: RNase P plate verification run failed
1. Remove the RNase P plate:
a. Press the tray to open it.
b. Remove the RNase P plate from the tray.
c. Push the tray back into the instrument.
2. Hold the plate up to a light source and verify that all wells
contain the same volume of fluid.
If there are differences in fluid volumes, check the heat seal of
the wells with lower volumes for signs of damage or
evaporation.
Also, compare the position of the wells that have lower
volumes with the outliers that you have removed from the
plate. If the well positions coincide, the heat seal on the plate
may be defective and resulted in the evaporation of the
associated samples.
3. Contact your Applied Biosystems service and sales
representative to order a replacement TaqMan RNase P
Instrument Verification Plate. If the replacement RNase P plate
fails, contact Applied Biosystems technical support or your
service representative for further assistance.
PHYSICAL INJURY HAZARD
.
During instrument operation, the sample block can be
heated to 100 °C. Before performing the following
procedure, be sure to wait until the sample block
reaches room temperature.
